scholarly journals NETs analysed by novel calprotectin‐based assays in blood donors and patients with multiple myeloma or rheumatoid arthritis: A pilot study

2020 ◽  
Vol 91 (5) ◽  
Author(s):  
Magne Kristoffer Fagerhol ◽  
Egil Johnson ◽  
Jon‐Magnus Tangen ◽  
Ivana Hollan ◽  
Mohammad Reza Mirlashari ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Multiple Myeloma ◽  
Pilot Study ◽  
Blood Donors

The Use of Radioiodinated Congo Red in the Study of Amyloidosis

1963 ◽  
Vol 03 (01) ◽  
pp. 25-38
Author(s):  
Manuel Tubis ◽  
William Blahd ◽  
John Endow
Keyword(s):  
Rheumatoid Arthritis ◽  
Multiple Myeloma ◽  
Comparative Studies ◽  
Congo Red ◽  
Half Time ◽  
Positive Correlation ◽  
Autopsy Findings ◽  
Percentage Removal

SummaryA study of the removal of I131-labeled Congo red from the blood of amyloid, non-amyloid, multiple myeloma, rheumatoid arthritis and other patients is presented. The percentage removal of the labeled dye shows the same variation reported by many other workers using Bennhold’s test and its modifications.However, there seems to be a positive correlation between the percentage removal of the labeled dye and the presence of amyloid as revealed by biopsy and autopsy. The half-time of disappearance is also correlated with the amyloidosis.The availability of the I131-labeled dye permits the use of very small weights of the dye thereby drastically reducing the possibility of toxic and sometimes fatal reactions encountered with the unlabeled dye. The I131 present permits easy quantitation of the dye in the blood without separation of plasma and obviates the need of fasting. It also permits external counting and scanning of deposits in the organs containing the dye.The availability and use of the labeled dye may stimulate more comparative studies of the removal of the dye from the blood correlated with biopsy and autopsy findings.

scholarly journals AB0008 THE IMPACT OF STAT4 RS7574865, IL6 RS1800795, IL6R RS2228145 AND RS4845618 ON RHEUMATOID ARTHRITIS SUSCEPTIBILITY IN BELARUSIAN POPULATION

2020 ◽  
Vol 79 (Suppl 1) ◽  
pp. 1307.1-1308
Author(s):  
E. Siniauskaya ◽  
T. Kuzhir ◽  
V. Yagur ◽  
R. Goncharova
Keyword(s):  
Rheumatoid Arthritis ◽  
Blood Donors ◽  
Genetic Model ◽  
Online Tool ◽  
Genotype Frequencies ◽  
Systemic Disorder ◽  
Autoimmune Pathogenesis ◽  
The Impact ◽  
Arthritis Susceptibility

Background:Rheumatoid arthritis (RA) is a chronic systemic disorder of the connective tissue of still unknown aetiology and complex autoimmune pathogenesis that primarily affects small joints. HLA alleles provide for 11-37% of the RA heritability, suggesting the substantial role of the non-HLA loci in genetic predisposition to RA. Among non-HLA loci,IL6, IL6RandSTAT4genes attract attention, however, the data concerning their influence on RA risk are somewhat contradictory.Objectives:The aim of the study was to analyze the involvement of four SNPs (STAT4rs7574865,IL6rs1800795,IL6Rrs2228145 and rs4845618) in RA susceptibility.Methods:187 patients diagnosed with RA (mean age 58.2 ± 11.9), and 380 healthy blood donors (mean age 37.18 ± 10.69 years) were included into the study. DNA extraction from peripheral blood samples was performed using the phenol-chloroform method. SNPs were genotyped using the real-time PCR with fluorescent probes. The allele and genotype frequencies were compared using the χ2 test. Odds ratios (ORs) and 95% confidence intervals (95% CIs) were calculated using the VassarStats online tool.Results:Utilizing recessive genetic model we found an association between TT genotype ofSTAT4rs7574865 (OR = 2.362; 95%CI [1.0378 – 5.376], p = 0.038) and RA. ForIL6rs1800795, it was found that CC genotype had significantly higher frequency among patients with rheumatoid arthritis as compared to that in controls (OR = 1.52; 95%CI [1.02 – 2.27], p = 0.0456). No associations ofIL6Rrs2228145 and rs4845618 SNPs with risk of RA were found in the total group of patients vs. controls. It was also shown thatIL6rs1800795 CC genotype frequency was significantly higher among the patients with RF-negative status (p = 0.0019).Conclusion:Thus, we provide evidence for association of theSTAT4rs7574865 andIL6rs1800795 variants with risk of RA in the Belarusian population, some features of interplay being revealed between gene polymorphisms analyzed and RA antibody status. Abovementioned SNPs may contribute to RA genetic susceptibility in the Belarusian population.Disclosure of Interests:None declared

AB1078-HPR Telephone Follow-Up, Standardized To The Initiation of Biologic Therapy of Patients with Rheumatoid Arthritis (RA) in A Specific Unit of Biologic Therapy. Pilot Study

2016 ◽  
Vol 75 (Suppl 2) ◽  
pp. 1300.2-1300 ◽  
Author(s):  
L. Cano-Garcia ◽  
S. Manrique-Arija ◽  
I. Ureña ◽  
N. Mena-Vazquez ◽  
M.C. Ordoñez-Cañizares ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Pilot Study ◽  
Biologic Therapy ◽  
Specific Unit

scholarly journals A Pilot Study on Zinc Levels in Patients with Rheumatoid Arthritis

2011 ◽  
Vol 143 (2) ◽  
pp. 854-862 ◽  
Author(s):  
Artur Mierzecki ◽  
Dorota Strecker ◽  
Krystyna Radomska
Keyword(s):  
Rheumatoid Arthritis ◽  
Pilot Study ◽  
Zinc Levels

A family-centered intervention for the transition to living with multiple myeloma as a chronic illness: A pilot study

2017 ◽  
Vol 35 ◽  
pp. 86-89 ◽  
Author(s):  
Susan R. Mazanec ◽  
Sarah Miano ◽  
Linda Baer ◽  
Erica L. Campagnaro ◽  
Abdus Sattar ◽  
...  
Keyword(s):  
Multiple Myeloma ◽  
Chronic Illness ◽  
Pilot Study ◽  
Family Centered

A2.18 Induction and characterisation of the dominant IL-10 producing B cell subset in healthy blood donors and rheumatoid arthritis patients

2016 ◽  
Vol 75 (Suppl 1) ◽  
pp. A22.2-A22
Author(s):  
ZS Bankó ◽  
J Pozsgay ◽  
M Tóth ◽  
T Gáti ◽  
G Nagy ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
B Cell ◽  
Blood Donors ◽  
Cell Subset

scholarly journals Use of the Dexamethasone-Corticotrophin Releasing Hormone Test to Assess Hypothalamic-Pituitary-Adrenal Axis Function in Rheumatoid Arthritis

2009 ◽  
Vol 2009 ◽  
pp. 1-6 ◽  
Author(s):  
Eman A. Hasan ◽  
David S. Jessop ◽  
Lynsey L. Power ◽  
Paul T. Monk ◽  
John R. Kirwan
Keyword(s):  
Rheumatoid Arthritis ◽  
Pilot Study ◽  
Plasma Cortisol ◽  
Hypothalamic Pituitary Adrenal Axis ◽  
Plasma Samples ◽  
Noninvasive Technique ◽  
Hypothalamic Pituitary Adrenal ◽  
Releasing Hormone ◽  
Adrenal Axis ◽  
Pituitary Adrenal Axis

Objectives. Hypothalamic-Pituitary-Adrenal axis function may be abnormal in rheumatoid arthritis (RA). A pilot study in 7 patients suggested impaired glucocorticoid feedback in some patients after the dexamethasone-corticotrophin releasing hormone (CRH) test. This study aimed to investigate the dexamethasone-corticotrophin releasing factor test in a larger group of patients and relate the results to characteristics of the disease.Methods. Outpatients with active RA (≥3 swollen and tender joints and C-reactive protein > 10 mg/L) took dexamethasone (1.5 mg) at 23:00 hour in the evening. Next day, baseline saliva and plasma samples were collected, CRH was infused at 11:00 hour, and 4 serial blood and saliva samples were collected. Plasma samples were stored at−80∘Cand a radioimmunoassay performed for saliva and plasma cortisol.Results. All 20 participants showed normal dexamethasone suppression and mounted no response to the CRH challenge. In samples with measurable cortisol, there was a strong correlation between saliva and plasma values (r= 0.876,n= 26,P<.01).Conclusion. No abnormalities were found in the Dexamethasone-CRH test in RA patients in contrast to a previous pilot study. Salivary cortisol measurement may offer an alternative noninvasive technique to plasma cortisol in RA patients in future studies.

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