Repellent action and contact toxicity mechanisms of the essential oil extracted from Chinese chive against Plutella xylostella larvae

2018 ◽  
Vol 100 (1) ◽  
pp. e21509 ◽  
Author(s):  
Quan Gao ◽  
Li Song ◽  
Jia Sun ◽  
Hai‐Qun Cao ◽  
Likun Wang ◽  
...  
Keyword(s):  
Essential Oil ◽  
Plutella Xylostella ◽  
Contact Toxicity ◽  
Chinese Chive ◽  
Toxicity Mechanisms ◽  
Repellent Action

scholarly journals Isolation and Insecticidal Activity of Essential Oil from Artemisia lavandulaefolia DC. against Plutella xylostella

Toxins ◽  
2021 ◽  
Vol 13 (12) ◽  
pp. 842
Author(s):  
Xing Huang ◽  
Yulin Huang ◽  
Chunyue Yang ◽  
Tiantian Liu ◽  
Xing Liu ◽  
...  
Keyword(s):  
Biological Activity ◽  
Essential Oil ◽  
Insecticidal Activity ◽  
Plutella Xylostella ◽  
Chemical Constituents ◽  
Lethal Dose ◽  
Detoxification Enzymes ◽  
Contact Toxicity ◽  
Caryophyllene Oxide ◽  
Active Constituents

Many plants show significant biological activity against pests due to their unique chemical constituents. It is important to identify effective constituents for their development and utilization as botanical pesticides. Our previous study showed that Artemisia lavandulaefolia essential oil had biological activity against Plutella xylostella. Here, we isolated and identified the constituents of essential oil from A. lavandulaefolia by silica gel column chromatography. The main constituents identified were eucalyptol and caryophyllene oxide, and they were confirmed by gas chromatography–mass spectrometry (GC–MS). Eucalyptol and caryophyllene oxide showed strong contact toxicity against P. xylostella larvae after 24 h of application (Median lethal dose, LD50 = 76.97 μL/mL and 20.71 mg/mL. Furthermore, the two active constituents against P. xylostella adults showed significant fumigant activity (Mmedian lethal concentration, LC50 = 3.25 μL/L and 1.06 mg/L, respectively. Finally, we measured the detoxification enzymes and acetylcholinesterase of the larvae treated with active constituents. The eucalyptol-treated larvae displayed enhanced carboxylesterase (CarE) and glutathione-S-transferase (GST) activities in an in vivo experiment, but it was lower for acetylcholinesterase (AchE) activity. The activities of the CarE and GST significantly decreased when exposed to caryophyllene oxide. In general, the two active constituents, eucalyptol and caryophyllene oxide, showed high insecticidal activity, which demonstrates their potential to be used as natural insecticides.

Knockdown of Uridine Diphosphate Glucosyltransferase 86Dg Enhances Susceptibility of Tribolium castaneum (Coleoptera: Tenebrionidae) to Artemisia vulgaris (Asterales: Asteraceae) Essential Oil

2021 ◽  
Author(s):  
Shanshan Gao ◽  
Haidi Sun ◽  
Jiahao Zhang ◽  
Yonglei Zhang ◽  
Peipei Sun ◽  
...  
Keyword(s):  
Essential Oil ◽  
Tribolium Castaneum ◽  
Expression Profile ◽  
Fat Body ◽  
Detoxification Enzymes ◽  
Developmental Expression ◽  
Contact Toxicity ◽  
Uridine Diphosphate ◽  
Artemisia Vulgaris ◽  
Coleoptera Tenebrionidae

Abstract Uridine diphosphate glucosyltransferases (UGTs), which are phase II detoxification enzymes, are found in various organisms. These enzymes play an important role in the detoxification mechanisms of plant allelopathy and in insects. Artemisia vulgaris L. (Asterales: Asteraceae: Artemisia) essential oil has strong contact toxicity to Tribolium castaneum Herbst (Coleoptera: Tenebrionidae) larvae. However, the effect of A. vulgaris essential oil on UGTs is unclear. In this study, A. vulgaris essential oil was shown to significantly induce the expression of the TcUgt86Dg transcript. Furthermore, treatment of TcUgt86Dg-silenced individuals with A. vulgaris essential oil resulted in higher mortality than for the control individuals, indicating that TcUgt86Dg is involved in detoxification of A. vulgaris essential oil in T. castaneum. The developmental expression profile showed that the expression of TcUgt86Dg in late adults was higher than in other developmental stages. Furthermore, the expression profile in adult tissues revealed higher expression of TcUgt86Dg in the head, antenna, fat body, and accessory gland than in other tissues. These data show that TcUgt86Dg may be involved in the metabolism of exogenous toxins by T. castaneum; thus, our results have elucidated one possible mechanism of resistance to A. vulgaris essential oil and provide a theoretical basis for a control scheme for T. castaneum.

scholarly journals Antifeedant, contact toxicity and oviposition deterrent effects of phyllostine acetate and phyllostine isolated from the endophytic fungus Diaporthe miriciae against Plutella xylostella larvae

2019 ◽  
Vol 76 (4) ◽  
pp. 1541-1548 ◽  
Author(s):  
Pamoda B Ratnaweera ◽  
J M Nisansala M Jayasundara ◽  
H H M Suseema D Herath ◽  
David E Williams ◽  
Sasini U Rajapaksha ◽  
...  
Keyword(s):  
Plutella Xylostella ◽  
Endophytic Fungus ◽  
Oviposition Deterrent ◽  
Contact Toxicity

scholarly journals Contact Toxicity and Repellency of the Essential Oil from Bupleurum bicaule Helm against Two Stored Product Insects

2018 ◽  
Vol 2018 ◽  
pp. 1-8
Author(s):  
Xiao-Meng Wei ◽  
Shan-Shan Guo ◽  
Hua Yan ◽  
Xian-Long Cheng ◽  
Feng Wei ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Chemical Composition ◽  
Essential Oil ◽  
Chemical Compounds ◽  
Gas Chromatography Mass Spectrometry ◽  
Bornyl Acetate ◽  
Contact Toxicity ◽  
Lasioderma Serricorne ◽  
Stored Product Insects

Essential oils obtained from many plants showed various kinds of insecticidal properties; some of them have been considered as alternative insecticides for pest control. The present study was aimed at determining the chemical composition of the essential oil from the roots of Bupleurum bicaule Helm, as well as evaluating the contact and repellent activities of the oil and four identified compounds against Lasioderma serricorne and Liposcelis bostrychophila adults. The essential oil was extracted by hydrodistillation, and its components were analyzed by gas chromatography-mass spectrometry (GC-MS). 26 components were determined and the main compounds included trans-2-isopropylbicyclo[4.3.0]non-3-en-8-one (25.9%), 4,5-dimethyl-1,2,3,6,7,8,8a,8b-octahydrobiphenylene (23.5%), and 1,4-dimethoxy-2-tert-butylbenzene (4.3%). It was found that the essential oil exhibited contact toxicity against L. serricorne (LD50 = 11.91 μg/adult), but the contact toxicity against L. bostrychophila could not be observed. The essential oil also showed strong repellent activity against L. serricorne with percent repellency of 100% at 78.63 nl/cm2. Four chemical compounds, 1,4-dimethoxy-2-tert-butylbenzene, bornyl acetate, (2E,4E)-2,4-nonadienal, and β-bisabolene, exhibited various levels of bioactivities. The experimental results indicated that the essential oil of B. bicaule and its individual compounds could be used in insecticidal and repellent strategies for stored product insects.

scholarly journals Contact Toxicity and Repellency of the Main Components From the Essential Oil ofClausena anisum-olensAgainst Two Stored Product Insects

2015 ◽  
Vol 15 (1) ◽  
pp. 87 ◽  
Author(s):  
Chun Xue You ◽  
Hai Yan Jiang ◽  
Wen Juan Zhang ◽  
Shan Shan Guo ◽  
Kai Yang ◽  
...  
Keyword(s):  
Essential Oil ◽  
Contact Toxicity ◽  
Stored Product Insects ◽  
Main Components

scholarly journals Composition and Biological Potential of Essential Oil from Thelechitonia trilobata Growing in South Africa

2011 ◽  
Vol 6 (12) ◽  
pp. 1934578X1100601
Author(s):  
Jamie Peebles ◽  
Ephraim Gwebu ◽  
Opeoluwa Oyedeji ◽  
Sarah Nanyonga ◽  
Nokuthula Kunene ◽  
...  
Keyword(s):  
South Africa ◽  
Chemical Composition ◽  
Essential Oil ◽  
Invasive Plant ◽  
Toxicity Tests ◽  
Contact Toxicity ◽  
Agricultural Pests ◽  
Biological Potential ◽  
Germacrene D ◽  
Positive Results

Thelechitonia trilobata is regarded as a troublesome weed that grows to form a dense blanket over the soil preventing the growth of other crops in farmland. Although the plant is regarded as a notorious, invasive plant, its chemical composition and biological potential have not been reported. The essential oil was isolated from the fresh leaves of T. trilobata using hydrodistillation. α-Pinene (21.6%), α-phellendrene (21.0%), limonene (12.8%) and germacrene D (7.5%) were the major constituents of the oil. The essential oil was screened against agricultural pests. The anti-tick properties were tested on Ripicephalus e. ervertsi found on sheep, while repellency, fumigation, and contact toxicity tests were carried out with maize weevils. Except for the contact toxicity test, all other bioassays gave positive results.

Effect of Citrus reticulata Blanco Essential Oil on Cryptolestes ferrugineus (Stephens) Adults

2017 ◽  
Vol 80 (12) ◽  
pp. 2090-2093 ◽  
Author(s):  
Jianhua Lü
Keyword(s):  
Essential Oil ◽  
Insect Pest ◽  
Anhydrous Diethyl Ether ◽  
Contact Toxicity ◽  
Fumigant Toxicity ◽  
Citrus Reticulata ◽  
Cryptolestes Ferrugineus ◽  
Fumigant Activity ◽  
Citrus Reticulata Blanco ◽  
Exposure Dosage

ABSTRACT The rusty grain beetle, Cryptolestes ferrugineus (Stephens), is a major insect pest of stored products worldwide. In an effort to manage populations of C. ferrugineus in stored grains, the essential oil from Citrus reticulata Blanco peel was prepared by the Soxhlet method with anhydrous diethyl ether, and its contact activity, repellent activity, and fumigant activity were investigated against C. ferrugineus adults. C. reticulata peel essential oil had potent contact, repellent, and fumigant activity against C. ferrugineus adults, with activity significantly increasing with increasing exposure dosage during the same exposure time. Percent repellency values achieved >80.0% after 60 h of exposure. For contact toxicity, the corrected mortality of C. ferrugineus adults reached >99.0% after 48 h of exposure at the dosage of 2.0 μL/cm2. For fumigant toxicity, the corrected mortality of C. ferrugineus adults reached 89.0 and 100.0% at the dosages of 40 and 80 μL/L of air, respectively. These results suggest that the C. reticulata peel essential oil has strong potential for managing populations of C. ferrugineus in practice.

scholarly journals Activity of Schinus Areira (Anacardiaceae) Essential Oils against the Grain Storage Pest Tribolium castaneum

2011 ◽  
Vol 6 (6) ◽  
pp. 1934578X1100600 ◽  
Author(s):  
Lilian R. Descamps ◽  
Carolina Sánchez Chopa ◽  
Adriana A. Ferrero
Keyword(s):  
Essential Oil ◽  
Essential Oils ◽  
Tribolium Castaneum ◽  
Feeding Deterrent ◽  
Contact Toxicity ◽  
Fumigant Toxicity ◽  
Nutritional Index ◽  
Leaf Essential Oil ◽  
Storage Pest ◽  
Coleoptera Tenebrionidae

Essential oils extracted from leaves and fruits of Schinus areira (Anacardiaceae) were tested for their repellent, toxic and feeding deterrent properties against Tribolium castaneum (Coleoptera: Tenebrionidae) larvae and adults. A topical application assay was employed for the contact toxicity study and filter paper impregnation for the fumigant assay. A treated diet was also used to evaluate the repellent activity and a flour disk bioassay for the feeding deterrent action and nutritional index alteration. The essential oil of the leaves contained mainly monoterpenoids, with α-phellandrene, 3-carene and camphene predominant, whereas that from the fruits contained mainly α-phellandrene, 3-carene and β-myrcene. The leaf essential oil showed repellent effects, whereas that from the fruit was an attractant. Both oils produced mortality against larvae in topical and fumigant bioassays, but fumigant toxicity was not found against adults. Moreover, both essential oils produced some alterations in nutritional index. These results show that the essential oils from S. areira could be applicable to the management of populations of Tribolium castaneum.

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