Knockdown of Uridine Diphosphate Glucosyltransferase 86Dg Enhances Susceptibility of Tribolium castaneum (Coleoptera: Tenebrionidae) to Artemisia vulgaris (Asterales: Asteraceae) Essential Oil

2021 ◽  
Author(s):  
Shanshan Gao ◽  
Haidi Sun ◽  
Jiahao Zhang ◽  
Yonglei Zhang ◽  
Peipei Sun ◽  
...  
Keyword(s):  
Essential Oil ◽  
Tribolium Castaneum ◽  
Expression Profile ◽  
Fat Body ◽  
Detoxification Enzymes ◽  
Developmental Expression ◽  
Contact Toxicity ◽  
Uridine Diphosphate ◽  
Artemisia Vulgaris ◽  
Coleoptera Tenebrionidae

Abstract Uridine diphosphate glucosyltransferases (UGTs), which are phase II detoxification enzymes, are found in various organisms. These enzymes play an important role in the detoxification mechanisms of plant allelopathy and in insects. Artemisia vulgaris L. (Asterales: Asteraceae: Artemisia) essential oil has strong contact toxicity to Tribolium castaneum Herbst (Coleoptera: Tenebrionidae) larvae. However, the effect of A. vulgaris essential oil on UGTs is unclear. In this study, A. vulgaris essential oil was shown to significantly induce the expression of the TcUgt86Dg transcript. Furthermore, treatment of TcUgt86Dg-silenced individuals with A. vulgaris essential oil resulted in higher mortality than for the control individuals, indicating that TcUgt86Dg is involved in detoxification of A. vulgaris essential oil in T. castaneum. The developmental expression profile showed that the expression of TcUgt86Dg in late adults was higher than in other developmental stages. Furthermore, the expression profile in adult tissues revealed higher expression of TcUgt86Dg in the head, antenna, fat body, and accessory gland than in other tissues. These data show that TcUgt86Dg may be involved in the metabolism of exogenous toxins by T. castaneum; thus, our results have elucidated one possible mechanism of resistance to A. vulgaris essential oil and provide a theoretical basis for a control scheme for T. castaneum.

scholarly journals Odorant Binding Protein C17 Contributes to the Response to Artemisia vulgaris Oil in Tribolium castaneum

2021 ◽  
Vol 3 ◽  
Author(s):  
Shan-Shan Gao ◽  
Rui-Min Li ◽  
Shuang Xue ◽  
Yuan-Chen Zhang ◽  
Yong-Lei Zhang ◽  
...  
Keyword(s):  
Tribolium Castaneum ◽  
Expression Profile ◽  
Developmental Stages ◽  
Fat Body ◽  
Profile Analysis ◽  
Control Group ◽  
Contact Toxicity ◽  
Artemisia Vulgaris ◽  
Plant Oil ◽  
Odorant Binding

The red flour beetle, Tribolium castaneum (T. castaneum), generates great financial losses to the grain storage and food processing industries. Previous studies have shown that essential oil (EO) from Artemisia vulgaris (A. vulgaris) has strong contact toxicity to larvae of the beetle, and odorant-binding proteins (OBPs) contribute to the defense of larvae against A. vulgaris. However, the functions of OBPs in insects defending against plant oil is still not clear. Here, expression of one OBP gene, TcOBPC17, was significantly induced 12–72 h after EO exposure. Furthermore, compared to the control group, RNA interference (RNAi) against TcOBPC17 resulted in a higher mortality rate after EO treatment, which suggests that TcOBPC17 involves in the defense against EO and induces a declining sensitivity to EO. In addition, the tissue expression profile analysis revealed that the expression of TcOBPC17 was more abundant in the metabolic detoxification organs of the head, fat body, epidermis, and hemolymph than in other larval tissue. The expression profile of developmental stages showed that TcOBPC17 had a higher level in early and late adult stages than in other developmental stages. Taken together, these results suggest that TcOBPC17 could participate in the sequestration process of exogenous toxicants in T. castaneum larvae.

scholarly journals Activity of Schinus Areira (Anacardiaceae) Essential Oils against the Grain Storage Pest Tribolium castaneum

2011 ◽  
Vol 6 (6) ◽  
pp. 1934578X1100600 ◽  
Author(s):  
Lilian R. Descamps ◽  
Carolina Sánchez Chopa ◽  
Adriana A. Ferrero
Keyword(s):  
Essential Oil ◽  
Essential Oils ◽  
Tribolium Castaneum ◽  
Feeding Deterrent ◽  
Contact Toxicity ◽  
Fumigant Toxicity ◽  
Nutritional Index ◽  
Leaf Essential Oil ◽  
Storage Pest ◽  
Coleoptera Tenebrionidae

Essential oils extracted from leaves and fruits of Schinus areira (Anacardiaceae) were tested for their repellent, toxic and feeding deterrent properties against Tribolium castaneum (Coleoptera: Tenebrionidae) larvae and adults. A topical application assay was employed for the contact toxicity study and filter paper impregnation for the fumigant assay. A treated diet was also used to evaluate the repellent activity and a flour disk bioassay for the feeding deterrent action and nutritional index alteration. The essential oil of the leaves contained mainly monoterpenoids, with α-phellandrene, 3-carene and camphene predominant, whereas that from the fruits contained mainly α-phellandrene, 3-carene and β-myrcene. The leaf essential oil showed repellent effects, whereas that from the fruit was an attractant. Both oils produced mortality against larvae in topical and fumigant bioassays, but fumigant toxicity was not found against adults. Moreover, both essential oils produced some alterations in nutritional index. These results show that the essential oils from S. areira could be applicable to the management of populations of Tribolium castaneum.

Toxicity and repellency of origanum essential oil and its components against Tribolium castaneum (Coleoptera: Tenebrionidae) adults

2010 ◽  
Vol 13 (4) ◽  
pp. 369-373 ◽  
Author(s):  
Soon-Il Kim ◽  
June-Sun Yoon ◽  
Je Won Jung ◽  
Ki-Bae Hong ◽  
Young-Joon Ahn ◽  
...  
Keyword(s):  
Essential Oil ◽  
Tribolium Castaneum ◽  
Coleoptera Tenebrionidae

scholarly journals Chemical constituents and biological activities against Tribolium castaneum (Herbst) of the essential oil from Citrus wilsonii leaves

2014 ◽  
Vol 79 (10) ◽  
pp. 1213-1222 ◽  
Author(s):  
Hai Chen ◽  
Kai Yang ◽  
Chun You ◽  
Shu Du ◽  
Qian Cai ◽  
...  
Keyword(s):  
Essential Oil ◽  
Tribolium Castaneum ◽  
Biological Activities ◽  
Chemical Constituents ◽  
Positive Control ◽  
Contact Toxicity ◽  
Fumigant Toxicity ◽  
Active Compounds ◽  
Active Constituents ◽  
Main Components

The essential oil obtained from Citrus wilsonii Tanaka leaves with hydrodistillation was investigated by GC and GC-MS. The main components of the essential oil were identified to be citronellol (16.94%), nerol acetate (10.35%), ?-terpinen (9.85%), citronellal (9.36%) and ?-pinene (6.72%). Among them, the four active constituents, predicted with a bioactivity-test, were isolated and identified as citronellol, ?-terpinene, nerol (neryl) acetate and ?-pinene. It was found that the essential oil of C. wilsonii leaves and isolated compounds possessed fumigant and contact toxicity against Tribolium castaneum adults. The essential oil and ?-terpinen showed strong fumigant toxicity against T. castaneum (LC50 = 8.18 and 4.09 mg L-1). Repellency of the crude oil and active compounds was also determined. Citronellol, neryl acetate and ?-pinene were strongly repellent (100%, 86% and 92%, respectively, at 78.63 nL cm-2, after 2 h treatment) against T. castaneum. The essential oil and citronellol exhibited the same level of repellency compared with the positive control, DEET. The results indicate that the essential oil of C. wilsonii leaves and its active compounds had the potential to be developed as natural fumigants, insecticides and repellents for control of T. castaneum.

scholarly journals Chemical Constituents of the Essential Oil Extracted from Elsholtzia densa and Their Insecticidal Activity against Tribolium castaneum and Lasioderma serricorne

Foods ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 2304
Author(s):  
Junyu Liang ◽  
Yazhou Shao ◽  
Haoshu Wu ◽  
Yue An ◽  
Junlong Wang ◽  
...  
Keyword(s):  
Essential Oil ◽  
Tribolium Castaneum ◽  
Insecticidal Activity ◽  
Chemical Constituents ◽  
Contact Toxicity ◽  
Fumigant Toxicity ◽  
Lasioderma Serricorne ◽  
Storage Pests ◽  
Insecticidal Toxicity ◽  
Different Levels

Storage pests pose a great threat to global food security. Here, we found that the essential oil (EO) extracted from E. densa possesses obvious effects against the insects that threaten stored-products. In this work, we investigated the chemical constituents of the essential oil extracted from Elsholtzia densa, and their insecticidal (contact and fumigant) toxicity against Tribolium castaneum and Lasioderma serricorne. A total of 45 compounds were identified by GC-MS, accounting for 98.74% of the total EO. Meanwhile, 11 compounds were isolated from the EO, including limonene, β-caryophyllene, ρ-cymene, trans-phytol, α-terpineol, linalool, acetophenone, 1,8-cineole, ρ-cymen-7-ol, 1-O-cerotoylgly-cerol, and palmitic acid. Furthermore, acetophenone, ρ-cymen-7-ol, and 1-O-cerotoylgly-cerol were isolated for the first time from Elsholtzia spp. The results of the bioassays indicated that the EO had the property of insecticidal toxicity against T. castaneum and L. serricorne. All of the compounds showed different levels of insecticidal toxicity against the two species of insects. Among them, 2-ethyl-1H-imidazole had no insecticidal toxicity against T. castaneum, but possessed fumigant and obvious contact toxicity against L. serricorne. ρ-Cymen-7-ol had beneficial insecticidal toxicity against the two species of insects, and fumigant toxicity against L. serricorne. ρ-Cymen-7-ol (LD50 = 13.30 μg/adult), 1-octen-3-ol (LD50 = 13.52 μg/adult), and 3-octanol (LD50 = 17.45 μg/adult) showed significant contact toxicity against T. castaneum. Acetophenone (LD50 = 7.07 μg/adult) and ρ-cymen-7-ol (LD50 = 8.42 μg/adult) showed strong contact toxicity against L. serricorne. ρ-Cymene (LC50 = 10.91 mg/L air) and ρ-cymen-7-ol (LC50 = 10.47 mg/L air) showed powerful fumigant toxicity to T. castaneum. Limonene (LC50 = 5.56 mg/L air), acetophenone (LC50 = 5.47 mg/L air), and 3-octanol (LC50 = 5.05 mg/L air) showed obvious fumigant toxicity against L. serricorne. In addition, the EO and its chemical compounds possessed different levels of repellent activity. This work provides some evidence of the value of exploring these materials for insecticidal activity, for human health purposes. We suggest that the EO extracted from E. densa may have the potential to be developed as an insecticidal agent against stored product insect pests.

Sign in / Sign up

Export Citation Format

Share Document