Inhibition by cycloheximide of ACTH-induced internal differentiation of mitochondria in cortical cells in tissue cultures of fetal rat adrenals

1971 ◽  
Vol 171 (1) ◽  
pp. 53-79 ◽  
Author(s):  
Arvi I. Kahri
Keyword(s):  
Tissue Cultures ◽  
Cortical Cells ◽  
Fetal Rat ◽  
Rat Adrenals

scholarly journals Effects of 5-bromodeoxyuridine on the ACTH-dependent mitochondrial biogenesis in cortical cells of fetal rat adrenals in tissue culture.

1976 ◽  
Vol 71 (3) ◽  
pp. 951-956 ◽  
Author(s):  
A I Kahri ◽  
M Salmenperä ◽  
A Saure
Keyword(s):  
Tissue Culture ◽  
Mitochondrial Dna ◽  
Dna Synthesis ◽  
Nuclear Dna ◽  
Acth Stimulation ◽  
Cortical Cells ◽  
Proliferative Phase ◽  
Fetal Rat ◽  
Rat Adrenals ◽  
Mitochondrial Dna Synthesis

Cortical cells of fetal rat adrenals in tissue culture were treated with 5-bromodeoxyuridine (BrdU) during their proliferative phase and during ACTH stimulation when nuclear DNA synthesis has almost ceased. Pretreatment with 0.5 mug/ml/day of BrdU inhibited the ACTH-induced differentiation of cortical cells as well as the secretion of corticosterone and 18-OH-deoxycorticosterone (18-OHDOC). When nuclear DNA synthesis was suppressed and mitochondrial DNA synthesis was stimulated by ACTH BrdU addition (30 mug/ml/day) permitted normal untrastructural differentiation of cortical cells, except that the development of mitochondrial inner membranes was inhibited. Simultaneously mitochondrial inner membranes was inhibited. Simultaneously mitochondrial 11beta- and 18-hydroxylations were strongly inhibited while cytoplasmic 21-hydroxylation was not affected.

EFFECTS OF CORTICOSTERONE ON ADRENOCORTICOTROPHIN-INDUCED MITOCHONDRIAL DIFFERENTIATION WITH SPECIAL REFERENCE TO 11β-AND 18-HYDROXYLATION

1976 ◽  
Vol 70 (2) ◽  
pp. 215-222 ◽  
Author(s):  
M. SALMENPERÄ ◽  
A. I. KAHRI ◽  
A. SAURE
Keyword(s):  
Significant Inhibition ◽  
Dibutyryl Cyclic Amp ◽  
Adrenocortical Cells ◽  
Cortical Cells ◽  
Foetal Rat ◽  
Rat Adrenals ◽  
Induced Changes ◽  
Regulation Of Growth ◽  
Venous Plasma

SUMMARY The effects of corticosterone in concentrations found in adrenal venous plasma on ACTH-induced changes in cultured cortical cells derived from foetal rat adrenals were studied. Corticosterone at a concentration of 5·7 × 10−5 mol/l completely inhibited mitochondrial differentiation to fasciculate-like morphology. The same cultures revealed significant inhibition of 11β- and 18-hydroxylation compared with cultures treated with ACTH only. This was shown by the reduced formation of corticosterone and 18-OH-deoxycorticosterone (48%, P < 0·001) and simultaneous enhancement of deoxycorticosterone formation (33%, P < 0·05) from added [4-14C]progesterone. Similar inhibition was observed when dibutyryl cyclic AMP replaced ACTH as an inducer of differentiation. Lower concentrations of corticosterone (1·2 × 10−5 and 2·4 × 10−5 mol/l) inhibited ACTH-stimulated formation of corticosterone and 18-OH-deoxycorticosterone from endogenous precursors. The results demonstrate that corticosterone regulates the stage of differentiation in cultured adrenocortical cells. The possible role of corticosterone in the regulation of growth and steroidogenic capacity of the adrenal cortex is discussed.

scholarly journals Effects of 3-isobutyryl-2-isopropylpyrazolo-(1,5-a)-pyridine(Ibudilast) on hypoxic injury in fetal rat cortical cells.

Nosotchu ◽  
1994 ◽  
Vol 16 (5) ◽  
pp. 354-359
Author(s):  
Takayuki Sakaki ◽  
Kazuo Yamada ◽  
Hideo Otsuki ◽  
Takamichi Yuguchi ◽  
Toru Hayakawa
Keyword(s):  
Cortical Cells ◽  
Hypoxic Injury ◽  
Fetal Rat

Norepinephrine Methylation in Fetal Rat Adrenals

Science ◽  
1966 ◽  
Vol 154 (3746) ◽  
pp. 275-276 ◽  
Author(s):  
F. L. Margolis ◽  
J. Roffi ◽  
A. Jost
Keyword(s):  
Fetal Rat ◽  
Rat Adrenals

Involvement of VIP on BDNF-induced somatostatin gene expression in cultured fetal rat cerebral cortical cells

2001 ◽  
Vol 94 (1-2) ◽  
pp. 59-66 ◽  
Author(s):  
Gemma Villuendas ◽  
Franco Sánchez-Franco ◽  
Nuria Palacios ◽  
Miriam Fernández ◽  
Lucinda Cacicedo
Keyword(s):  
Gene Expression ◽  
Cortical Cells ◽  
Fetal Rat
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