Inhibition of ACTH-induced differentiation of cortical cells and their mitochondria by corticosterone in tissue culture of fetal rat adrenals

1973 ◽  
Vol 176 (3) ◽  
pp. 253-271 ◽  
Author(s):  
Arvi I. Kahri
Keyword(s):  
Tissue Culture ◽  
Induced Differentiation ◽  
Cortical Cells ◽  
Fetal Rat ◽  
Rat Adrenals

scholarly journals Effects of 5-bromodeoxyuridine on the ACTH-dependent mitochondrial biogenesis in cortical cells of fetal rat adrenals in tissue culture.

1976 ◽  
Vol 71 (3) ◽  
pp. 951-956 ◽  
Author(s):  
A I Kahri ◽  
M Salmenperä ◽  
A Saure
Keyword(s):  
Tissue Culture ◽  
Mitochondrial Dna ◽  
Dna Synthesis ◽  
Nuclear Dna ◽  
Acth Stimulation ◽  
Cortical Cells ◽  
Proliferative Phase ◽  
Fetal Rat ◽  
Rat Adrenals ◽  
Mitochondrial Dna Synthesis

Cortical cells of fetal rat adrenals in tissue culture were treated with 5-bromodeoxyuridine (BrdU) during their proliferative phase and during ACTH stimulation when nuclear DNA synthesis has almost ceased. Pretreatment with 0.5 mug/ml/day of BrdU inhibited the ACTH-induced differentiation of cortical cells as well as the secretion of corticosterone and 18-OH-deoxycorticosterone (18-OHDOC). When nuclear DNA synthesis was suppressed and mitochondrial DNA synthesis was stimulated by ACTH BrdU addition (30 mug/ml/day) permitted normal untrastructural differentiation of cortical cells, except that the development of mitochondrial inner membranes was inhibited. Simultaneously mitochondrial inner membranes was inhibited. Simultaneously mitochondrial 11beta- and 18-hydroxylations were strongly inhibited while cytoplasmic 21-hydroxylation was not affected.

CORTICOTROPHIN-INDUCED DIFFERENTIATION OF MITOCHONDRIA IN RAT ADRENAL CORTICAL CELLS GROWN IN PRIMARY TISSUE CULTURE: EFFECTS OF ETHIDIUM BROMIDE

1972 ◽  
Vol 52 (3) ◽  
pp. 541-NP ◽  
Author(s):  
A. JOSEPHINE MILNER
Keyword(s):  
Tissue Culture ◽  
Ethidium Bromide ◽  
Cortical Cell ◽  
Morphological Transformation ◽  
Inner Mitochondrial Membrane ◽  
Induced Differentiation ◽  
Cortical Cells ◽  
Primary Tissue Culture ◽  
Culture Effects ◽  
Primary Tissue

SUMMARY Foetal rat adrenals were grown in primary tissue culture. The effects of ethidium bromide on the adrenocorticotrophin (ACTH)-induced differentiation of the mitochondria of the cortical cells were examined. Ethidium bromide (1 μg/ml/day for 4 days) inhibited the morphological transformation of the mitochondria normally induced by ACTH. At the same time the steroid 11β-hydroxylating activity, which is associated with the inner mitochondrial membrane, was either partially or completely blocked. The ethidium bromide-treated cells contained mitochondria of varying sizes and with poorly developed inner membranes. The biochemical and morphological effects of ethidium bromide on the cortical cell mitochondria were only partially reversible.

DIFFERENT BIOLOGICAL ACTION OF CORTICOSTEROIDS, CORTICOSTERONE AND CORTISOL, AS A BASE OF ZONAL FUNCTION OF ADRENAL CORTEX

1979 ◽  
Vol 91 (2) ◽  
pp. 329-337 ◽  
Author(s):  
A. I. Kahri ◽  
R. Voutilainen ◽  
M. Salmenperä
Keyword(s):  
Tissue Culture ◽  
Adrenal Gland ◽  
Primary Culture ◽  
Adrenal Cortex ◽  
Biological Action ◽  
Cortical Cells ◽  
Adrenal Steroidogenesis ◽  
Foetal Rat ◽  
Rat Adrenals ◽  
Aldosterone Production

ABSTRACT The effects of corticosterone and cortisol in concentrations attainable in the adrenal gland were studied on ACTH-induced steroidogenesis in cultured cortical cells of foetal human and rat adrenals. Corticosterone at a concentration of 5.8 × 10−5 mol/l clearly inhibited cortisol production (65.5%; P < 0.005) and simultaneously increased androgen production in tissue culture of foetal human adrenals. Cortisol at a concentration of 2.8 × 10−4 mol/l clearly inhibited 18-OH-DOC (74.0 %, P < 0.001) and aldosterone (83.7 % P < 0.005) production in tissue culture of foetal rat adrenals. In primary culture of foetal human adrenals cortisol did not decrease aldosterone production absolutely, but it significantly decreased the relative amount of aldosterone with respect to corticosterone. Cortisol did not inhibit corticosterone production in either culture. The results demonstrate that cortisol and corticosterone have qualitatively different effects on adrenal steroidogenesis and that these steroids may play a basic role in the functional zonation of the adrenal gland.

The Anabolic Effects of Progesterone on Fetal Rat Calvaria in Tissue Culture

1994 ◽  
Vol 1 (4) ◽  
pp. 302-309 ◽  
Author(s):  
Deborah L. Manzi ◽  
Carol C. Pilbeam ◽  
Lawrence G. Raisz
Keyword(s):  
Tissue Culture ◽  
Rat Calvaria ◽  
Fetal Rat
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