Selective staining of glycogen by ammoniacal silver nitrate: A new method

1944 ◽  
Vol 90 (4) ◽  
pp. 261-266 ◽  
Author(s):  
Arthur J. Mitchell ◽  
George B. Wislocki
Keyword(s):  
Silver Nitrate ◽  
New Method ◽  
Selective Staining ◽  
Ammoniacal Silver

scholarly journals SELECTIVE STAINING OF NERVOUS TISSUE FOR LIGHT MICROSCOPY FOLLOWING PREPARATION FOR ELECTRON MICROSCOPY

1968 ◽  
Vol 16 (12) ◽  
pp. 808-814 ◽  
Author(s):  
LILLIAN R. BERKOWITZ ◽  
OLGA FIORELLO ◽  
LAWRENCE KRUGER ◽  
DAVID S. MAXWELL
Keyword(s):  
Electron Microscopy ◽  
Cerebral Cortex ◽  
Silver Nitrate ◽  
Nervous Tissue ◽  
Cell Structure ◽  
Differential Staining ◽  
Selective Staining ◽  
Axon Terminals ◽  
Ammoniacal Silver ◽  
Good Contrast

The suitability and selectivity of several dyes have been tested on plastic-embedded sections of nervous tissue (0.5-1 µ) prepared for electron microscopy. Methods for plastic extraction and selective staining are described which provide satisfactory differentiation of principle cytologic features with good contrast and clarity of detail. The introduction of a hematoxylin "lake" has achieved the differential staining of oligodendroglia and astrocytes in cerebral cortex. The argyrophilia of cells and cell structure following 10-20% silver nitrate or silver nitrate in combination with ammoniacal silver permits identification of axon terminals.

scholarly journals Two Modes of Nanoleakage Expression in Single-step Adhesives

2002 ◽  
Vol 81 (7) ◽  
pp. 472-476 ◽  
Author(s):  
F.R. Tay ◽  
D.H. Pashley ◽  
M. Yoshiyama
Keyword(s):  
Silver Nitrate ◽  
Silver Ions ◽  
Single Step ◽  
Mineral Dissolution ◽  
Resin Infiltration ◽  
Transmission Electron ◽  
Hybrid Layers ◽  
Self Etch ◽  
Ammoniacal Silver ◽  
Spotted Pattern

Self-etch adhesives that etch, prime, and bond simultaneously should not exhibit incomplete resin infiltration within hybrid layers. We hypothesized that nanoleakage patterns in these systems are artifacts caused by mineral dissolution in mildly acidic silver nitrate. Resin-dentin interfaces bonded with four single-step, self-etch adhesives were examined for nanoleakage by conventional (pH 4.2) and basic ammoniacal (pH 9.5) silver nitrate and prepared for transmission electron microscopy. All adhesives exhibited a reticular mode of nanoleakage within hybrid layers when conventional silver nitrate was used. With ammoniacal silver nitrate, an additional spotted pattern of nanoleakage was observed within adhesive and hybrid layers. The reticular mode of nanoleakage in self-etch adhesives probably represents sites of incomplete water removal that leads to regional suboptimal polymerization. The spotted pattern identified with the use of ammoniacal silver nitrate probably represents potentially permeable regions in the adhesive and hybrid layers that result from the interaction of the basic diamine silver ions with acidic/hydrophilic resin components.

scholarly journals VARIABILITY OF THE SHAPE AND ARGENTAFFINITY OF THE GRANULES IN THE ENTEROENDOCRINE CELLS OF THE MOUSE DUODENUM

1974 ◽  
Vol 22 (10) ◽  
pp. 929-944 ◽  
Author(s):  
DAVID B. NICHOLS ◽  
HAZEL CHENG ◽  
C. P. LEBLOND
Keyword(s):  
Electron Microscope ◽  
Silver Nitrate ◽  
Wide Spectrum ◽  
Uranyl Acetate ◽  
Enteroendocrine Cells ◽  
Lead Citrate ◽  
A Cell ◽  
The Mean ◽  
Electron Microscopes ◽  
Ammoniacal Silver

The enteroendocrine cells of the mouse duodenum were examined in the light and electron microscopes by comparing Epon sections stained by Gomori's silver methenamine with adjacent sections stained by Masson's ammoniacal silver nitrate, iron hematoxylin or uranyl acetate and lead citrate. Furthermore, the granules present in the enteroendocrine cells stained with silver methenamine were investigated in the electron microscope: their shape was defined as either spherical or nonspherical and, in either case, their density was measured by cytophotometry. The comparison of adjacent sections of mouse duodenum by different techniques indicates that the granules of all enteroendocrine cells are stained by iron hematoxylin, whereas those of about 94% of the cells are stained by silver methenamine. Since silver methenamine stains exactly the same cells as ammoniacal silver nitrate, it may be used as a test of argentaffinity. It is concluded that about 94% of the enteroendocrine cells are argentaffin and about 6% are nonargentaffin. The argentaffin cells display a wide spectrum of granule shape. Nearly all of the granules of a cell may be spherical or nearly all nonspherical or, more commonly, there is a fair number of both kinds. In the few cells which are not argentaffin, over 70% of the granules are spherical. After silver methenamine staining, the mean density of spherical and nonspherical granules is statistically equal within any given cell. Yet, when cells are compared to one another, the mean density of the granules, and therefore the argentaffinity, increases with the proportion of nonspherical granules. It is concluded that, in the mouse duodenun, argentaffinity is most pronounced in the cells with irregular nonspherical granules, but it is by no means confined to such cells. Moreover, the argentaffinity as well as the shape of the granules varies within wide limits.

Influence of selective etching on the enamel and dentin leakage of three low shrinkage composites in class V restoration

2020 ◽  
Vol 10 (11) ◽  
pp. 1941-1949
Author(s):  
Hend N. Al-Nahedh
Keyword(s):  
Silver Nitrate ◽  
Selective Etching ◽  
Thermal Cycles ◽  
Class V ◽  
Bond Force ◽  
Selective Etch ◽  
Significant Difference ◽  
One Step ◽  
Self Etch ◽  
Ammoniacal Silver

This study determined if prior selective enamel etching affected leakage of enamel/dentin margins in Class V low-shrinkage resin-based composite restorations. Round cavities, centered on the cemento/enamel junction, 2 mm in diameter and 2 mm in depth were prepared on the buccal, palatal or lingual surfaces of premolar teeth to have a total of 60 cavities. One side of the tooth received selective enamel etching and both sides were restored using one of 3 restorative materials (n = 10): Aelite LS posterior/All-bond SE, Estelite Sigma Quick/Bond Force, or Filtek P90/P90 System Adhesive. The teeth were fatigued for 5000 thermal cycles, and the marginal sealing was challenged with 50% ammoniacal silver nitrate. The interface was analyzed and the leakage score was calculated. Two-way ANOVA revealed that Filtek P90 (25.5±17.4) had lower leakage than Estelite Sigma Quick (63.1±34.5) and Aelite LS Posterior (64.2±41.8) with self-etching. With selective etching, the three materials showed reduced leakage in enamel. A significant difference (P <0.05) was found between leakage in enamel and dentin for the Aelite LS Posterior selective etch group and for Estelite Sigma Quick. Selective etching reduced enamel leakage for all materials in one-step self-etch materials. Two-step self-etch adhesives bond better to enamel and dentin than one-step resin-based adhesives.

MICROCHROMOSOMES OF THE ONTARIO RED FOX (VULPES VULPES): AN ATTEMPT AT CHARACTERIZATION

1980 ◽  
Vol 22 (4) ◽  
pp. 553-567 ◽  
Author(s):  
J. A. Ellenton ◽  
P. K. Basrur
Keyword(s):  
Electron Microscopy ◽  
Silver Nitrate ◽  
Vulpes Vulpes ◽  
Red Fox ◽  
Light And Electron Microscopy ◽  
Testicular Tissue ◽  
S Phase ◽  
Mitotic Chromosomes ◽  
Autoradiographic Analysis ◽  
Ammoniacal Silver

Characterization of the microchromosomes of the red fox, Vulpes vulpes Linn., was attempted through the examination of mitotic chromosomes using Giemsa banding, quinacrine banding, the silver nitrate-ammoniacal silver technique for staining nucleolar organizers, and autoradiographic procedures. Pachytene cells were examined in air-dried and squash meiotic preparations and in testicular tissue sectioned for light and electron microscopy. The results of banding procedures on mitotic chromosomes and the staining properties of the microchromosomes at pachytene indicated that the microchromosomes likely contain both heterochromatin and euchromatin. Autoradiographic analysis showed that the microchromosomes replicate during mid S phase while the Y chromosome, which is in the size range of the microchromosomes, replicates during late S phase. From these observations, it would appear that the microchromosomes may not be exclusively heterochromatic as hypothesized previously. With the use of the silver nitrate-ammoniacal silver technique, the presence of nucleolar regions were detected on specific macrochromosomes but not on any of the microchromosomes. Examination of pachytene chromosomes in air-dried and squash preparations, and of testicular tissue sectioned for light and electron microscopy, also indicated that the microchromosomes may not be involved in the organization of the nucleolus in the red fox.

The pigmentation of the jellyfish, Pelagia noctiluca (Forskål) var. panopyra Péron & Lesueur

1954 ◽  
Vol 142 (908) ◽  
pp. 392-408 ◽  
Keyword(s):  
Amino Acids ◽  
Silver Nitrate ◽  
Absorption Maximum ◽  
Pelagia Noctiluca ◽  
Wide Range ◽  
Group A ◽  
Phenolic Group ◽  
Pass Through ◽  
Hydrolysis Of ◽  
Ammoniacal Silver

Four or more pigm ents, viz. at least two blue, a brown and a m agenta, have been found in Pelagia noctiluca var. panopyra . One blue occurs in the lips, a similar one in the exumbrella, and another in the gonadial endoderm and the gastric filaments. The brown occurs in the ectoderm of the exumbrella, in the gonads and the m esogloea; the m agenta occurs in the mesogloea. The blues are intracellular, the m agenta extracellular, and th e brown both intracellular and extracellular. The pigm entary material occurs either as m inute free ellipsoids or spheroids, or in association w ith larger colourless bodies. The extracted pigm ents show absorption m axim a as follows: blue from the gonads and gastric filaments, 632·5, 590 and 560 m μ ; blue from the exumbrella, 545 to 550 m μ , and that from the lips, 537 to 547 m μ ; magenta, 490 to 498 m μ . Autolysis and hydrolysis of the blue gonadial and of the magenta pigment by alkali or by trypsin, shifts the absorption maxima toward the shorter wave-lengths. The magenta pigment was studied in detail, and its solubility, its behaviour towards denaturants and its incapacity to pass through a collodion membrane, suggest that it is a chromoprotein. The chromogen, precipitated from tryptic digests by alkali, showed an absorption maximum at 380 m μ ; it was insoluble in a wide range of solvents, and could be oxidized and reduced only by relatively drastic means. It reduced ammoniacal silver nitrate in the dark. It was shown to contain chlorine, but no sulphur or phosphorus; it contains organically combined nitrogen, but no protein or α-amino-acids. Various tests showed the presence of a substituted phenolic group, a pyrrole group, and the indole nucleus. It thus appears to be a melanoid. No evidence was obtained for the existence of an active tyrosinase.

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