scholarly journals VARIABILITY OF THE SHAPE AND ARGENTAFFINITY OF THE GRANULES IN THE ENTEROENDOCRINE CELLS OF THE MOUSE DUODENUM

1974 ◽  
Vol 22 (10) ◽  
pp. 929-944 ◽  
Author(s):  
DAVID B. NICHOLS ◽  
HAZEL CHENG ◽  
C. P. LEBLOND
Keyword(s):  
Electron Microscope ◽  
Silver Nitrate ◽  
Wide Spectrum ◽  
Uranyl Acetate ◽  
Enteroendocrine Cells ◽  
Lead Citrate ◽  
A Cell ◽  
The Mean ◽  
Electron Microscopes ◽  
Ammoniacal Silver

The enteroendocrine cells of the mouse duodenum were examined in the light and electron microscopes by comparing Epon sections stained by Gomori's silver methenamine with adjacent sections stained by Masson's ammoniacal silver nitrate, iron hematoxylin or uranyl acetate and lead citrate. Furthermore, the granules present in the enteroendocrine cells stained with silver methenamine were investigated in the electron microscope: their shape was defined as either spherical or nonspherical and, in either case, their density was measured by cytophotometry. The comparison of adjacent sections of mouse duodenum by different techniques indicates that the granules of all enteroendocrine cells are stained by iron hematoxylin, whereas those of about 94% of the cells are stained by silver methenamine. Since silver methenamine stains exactly the same cells as ammoniacal silver nitrate, it may be used as a test of argentaffinity. It is concluded that about 94% of the enteroendocrine cells are argentaffin and about 6% are nonargentaffin. The argentaffin cells display a wide spectrum of granule shape. Nearly all of the granules of a cell may be spherical or nearly all nonspherical or, more commonly, there is a fair number of both kinds. In the few cells which are not argentaffin, over 70% of the granules are spherical. After silver methenamine staining, the mean density of spherical and nonspherical granules is statistically equal within any given cell. Yet, when cells are compared to one another, the mean density of the granules, and therefore the argentaffinity, increases with the proportion of nonspherical granules. It is concluded that, in the mouse duodenun, argentaffinity is most pronounced in the cells with irregular nonspherical granules, but it is by no means confined to such cells. Moreover, the argentaffinity as well as the shape of the granules varies within wide limits.

Unusual Intranuclear Structures in Chick Sympathetic Neurons

1967 ◽  
Vol 25 ◽  
pp. 188-189
Author(s):  
E. B. Masurovsky ◽  
H. H. Benitez ◽  
M. R. Murray
Keyword(s):  
Electron Microscope ◽  
Sympathetic Neurons ◽  
Uranyl Acetate ◽  
Sympathetic Ganglia ◽  
Lead Citrate ◽  
Thin Sections ◽  
Cns Neurons ◽  
Mammalian Cns

Recent light- and electron microscope studies concerned with the effects of D2O on the development of chick sympathetic ganglia in long-term, organized culture revealed the presence of rod-like fibrillar formations, and associated granulofibrillar bodies, in the nuclei of control and deuterated neurons. Similar fibrillar formations have been reported in the nuclei of certain mammalian CNS neurons; however, related granulofibrillar bodies have not been previously described. Both kinds of intranuclear structures are observed in cultures fixed either in veronal acetate-buffered 2%OsO4 (pH 7. 4), or in 3.5% glutaraldehyde followed by post-osmication. Thin sections from such Epon-embedded cultures were stained with ethanolic uranyl acetate and basic lead citrate for viewing in the electron microscope.

Fine Structure of Interdental Cells in the Mouse Cochlea

1970 ◽  
Vol 28 ◽  
pp. 140-141
Author(s):  
Roberta M. Bruck
Keyword(s):  
Fine Structure ◽  
Electron Microscope ◽  
Young Adult ◽  
Uranyl Acetate ◽  
Ground Substance ◽  
Tectorial Membrane ◽  
Lead Citrate ◽  
Unusual Structure ◽  
Thin Sections ◽  
Collagenous Fibers

An unusual structure in the cochlea is the spiral limbus; this periosteal tissue consists of stellate fibroblasts and collagenous fibers embedded in a translucent ground substance. The collagenous fibers are arranged in vertical columns (the auditory teeth of Haschke). Between the auditory teeth are interdental furrows in which the interdental cells are situated. These epithelial cells supposedly secrete the tectorial membrane.The fine structure of interdental cells in the rat was reported by Iurato (1962). Since the mouse appears to be different, a description of the fine structure of mouse interdental cells' is presented. Young adult C57BL/6J mice were perfused intervascularly with 1% paraformaldehyde/ 1.25% glutaraldehyde in .1M phosphate buffer (pH7.2-7.4). Intact cochlea were decalcified in .1M EDTA by the method of Baird (1967), postosmicated, dehydrated, and embedded in Araldite. Thin sections stained with uranyl acetate and lead citrate were examined in a Phillips EM-200 electron microscope.

Divisions cellulaires au niveau de l'épiderme de l'hypocotyle du lin (Linum usitatissimum) cultivé in vitro

1985 ◽  
Vol 63 (10) ◽  
pp. 1691-1695 ◽  
Author(s):  
M. Sqalli ◽  
H. Chlyah
Keyword(s):  
Cell Division ◽  
Culture Cell ◽  
Epidermal Cells ◽  
Isolated Cells ◽  
Initiation And Propagation ◽  
A Cell ◽  
The Mean ◽  
Electron Microscopes ◽  
Scanning Electron Microscopes

A study of the initiation and propagation of cell divisions in the epidermis of flax hypocotyl segments cultured in vitro was made using surface observations (light and scanning electron microscopes) as well as transverse and longitudinal sections. Epidermal cells were of two types: long, narrow cells and short, wide cells. The latter, less numerous, rarely participated in cell division. Nuclear activation and the first mitoses appeared very early (after 4–8 h of culture). Cell division began in isolated cells and spread progressively to surrounding cells arranged transversely. At 24 h, approximately 50 cells in division or newly divided were observed on an epidermal strip of 10 × 2 mm composed of about 8000 original cells. At 48 h, about 110 cells had divided forming 22 division centers; 26 prophase, 10 metaphase, and 7 telophase figures were observed. The mean number of original cells which participated in the formation of a cell division center was three at 12 h, five at 72 h, with no increase thereafter. The percentage of cells in mitosis or already divided remained low (1.9%) in relation to the total number of epidermal cells. For 22 division centers, only 7 would participate in vegetative bud formation.

Ultrastructural Morphology of the Anagen Stage Hair Follicle of Male Beagle Dogs

1977 ◽  
Vol 35 ◽  
pp. 652-653
Author(s):  
F. A. Al-Bagdadi ◽  
C. W. Titkemeyer ◽  
J. E. Lovell
Keyword(s):  
Electron Microscope ◽  
Basement Membrane ◽  
Skin Biopsy ◽  
Osmium Tetroxide ◽  
Dermal Papilla ◽  
Uranyl Acetate ◽  
Ground Substance ◽  
Lead Citrate ◽  
Beagle Dogs ◽  
Cytoplasmic Processes

Skin biopsy samples were collected monthly from the lateral sides of 9 male Beagle dogs over a period of 1 year. The samples were fixed in 3% gluteral- dehyde and post fixed in 1% osmium tetroxide using phosphate or S-Collidine as buffers. They were dehydrated, embedded in Epon 812, sectioned with an LKB Ultrotome III, and stained with Reynolds' lead citrate and 1% uranyl acetate. They were examined and photographed by use of an RCA-3H electron microscope.The dermal papilla during anagen consisted of fibroblast-like cells some of which had cytoplasmic processes. The cytoplasm contained many mitochondria. (Fig. 1) The cells of the dermal papilla were either peripherally arranged spindle-shaped cells or polygonal cells. The basement membrane was either related to the fibroblast cytoplasmic processes or was free of the cytoplasmic processes but with a thick zone composed of fibers, granular ground substance and spaces.

Intracisternal microtubules in proximal tubule cells of duck kidney: A serendipitous finding

1987 ◽  
Vol 45 ◽  
pp. 898-899
Author(s):  
M.K. Bhatnagar ◽  
S.M. Snelgrove-Hobson ◽  
P.V.V. Prasada Rao
Keyword(s):  
Electron Microscope ◽  
Proximal Tubule ◽  
Osmium Tetroxide ◽  
Uranyl Acetate ◽  
Kidney Cortex ◽  
Lead Citrate ◽  
Cell Organelles ◽  
Thin Sections ◽  
Proximal Tubule Cells ◽  
Tubule Cells

Cytpplasmic microtubules, ubiquitous cell organelles, lie free in the cytosol without being compartmented off by membranes. In rare instances, however, intracisternal microtubules (ICM) have been reported in ganglionic neurons of aging dogs and in the proximal tubule cells (PTC) of rabbit kidneys. We report here the presence of ICM in the PTC of the kidneys of Peking ducks (Anas platyrhynhos).A total of 106 Peking ducks (24 males and 24 females of 9 months, 48 females of 15 months, 6 males of 30 months and 2 males and 2 females of 48 months of age) were anesthetized and exsanguinated. Pieces of kidney cortex were fixed in 4% glutaraldehyde in 0.5M phosphate buffer (pH 7.3) at 29°C for four hours. Samples were post fixed in 2% osmium tetroxide for two hours. One micron sections of Epon-embedded cortices were stained with toluidine blue and thin sections (70-70 nm) contrasted with uranyl acetate and lead citrate were examined with a JEOL 100-S electron microscope at 80 kV.

Bacterioids in the Rectal Organ of the Lantern Dug Pyrops Candelaria Linn (Homoptera: Fulgoridae)

1996 ◽  
Vol 54 ◽  
pp. 806-807
Author(s):  
W.W.K. Cheung ◽  
J.B. Wang
Keyword(s):  
Fine Structure ◽  
Electron Microscope ◽  
Osmium Tetroxide ◽  
Uranyl Acetate ◽  
Sodium Cacodylate Buffer ◽  
Lead Citrate ◽  
Sodium Cacodylate ◽  
Special Pair ◽  
Adult Females ◽  
Cacodylate Buffer

The lantern bug harbours three symbionts, namely a, x and i in its body. These microorganisms are supposed to be transmitted transovarially to the future progeny. The x-symbionts are found in a special pair of organs called the x-organ which bulges to form a rectal organ in adult females. The purpose of this study is to investigate into the fine structure of the x-symbionts. This will serve as a basis for understanding the interactions of this microorganism with its host.The rectum of the lantern bug Pyrops candelaria Linn, was dissected out in buffered insect saline and fixed in 2.5% glutaradehyde in 0.1M sodium cacodylate buffer (pH 7.2) for 1 hr. The rectal organ was subsequently post-fixed in 1% osmium tetroxide (pH 7.2) and dehydrated in alcohol/acetone series. These were blocked in Spurr resin and cut with a Reichert Ultratome. Sections were stained with uranyl acetate and lead citrate and examined with a JEOL JEM-1200EX electron microscope. Thick sections (1 μn) were stained with 1% toluidine blue and examined under a Nikon Optiphot light microscope.

Ultrastructure of Angiosarcoma of Mouse Tail

1980 ◽  
Vol 38 ◽  
pp. 740-741
Author(s):  
White Yvonne ◽  
Winslow Sheldon ◽  
James W. Townsend ◽  
Neil A. Littlefield
Keyword(s):  
Electron Microscopy ◽  
Electron Microscope ◽  
Light Microscopy ◽  
Toluidine Blue ◽  
Female Mouse ◽  
Osmium Tetroxide ◽  
Uranyl Acetate ◽  
Lead Citrate ◽  
Ultrathin Sections ◽  
Resin Mixture

Spontaneous neoplasms rarely occur on the tails of BALB/cStCrlfC3H/Nctr mice, but the neoplasm most frequently observed is a locally invasive non-metastatic angiosarcoma. In this case a female mouse weighing 33.2 g, 706 days of age, presented a soft, red, irregularly-shaped mass, measuring 18 mm in its greatest dimension, in the subcutis of the base of the tail. A portion of the tail tumor was taken for electron microscopy and the remainder was processed for light microscopy. The tissue processed for electron microscopy was fixed in 4% cacodyl ate-buffered glutaraldehyde, post-fixed in 1% osmium tetroxide, dehydrated in a graded series of ethanol solutions, and embedded in Epon-Araldite resin mixture. Sections of 1 μm were stained with toluidine blue for light microscopy and ultrathin sections of 100 nm were stained with uranyl acetate and lead citrate, then examined with a Philips EM201 electron microscope .

Ultrastructural Observations of Endometrial Hyaline Accumulation in Diethylstilbestrol-Treated Mice

1980 ◽  
Vol 38 ◽  
pp. 458-459
Author(s):  
R. Wordinger ◽  
B. Highman ◽  
J. Townsend ◽  
D. Greenman
Keyword(s):  
Electron Microscope ◽  
Osmium Tetroxide ◽  
Uranyl Acetate ◽  
Lead Citrate ◽  
Specific Pathogen Free ◽  
Experimental Animals ◽  
Estrogen Exposure ◽  
Feed Control ◽  
Specific Pathogen ◽  
Experimental Group

Several uterine lesions have been correlated with continuous estrogen exposure. Accumulation of a hyaline-like material has been described by light microscopy (1,2,3). The objective of this study was to describe the ultrastructure of this lesion in the mouse. All mice were of the BALB/cStCrl fC3H/Nctr strain and were specific-pathogen-free produced from cesarean-derived parentage. Experimental animals were fed diets containing 320 or 640 parts per billion (ppb) diethylsti 1 bestrol (DES) dissolved in 95% ethanol and mixed in the feed. Control animals received the same feed, but without DES. Five animals from each experimental group, were sacrificed by ether overdose when moribund, and ranged in age from 622 to 782 days. Tissues were fixed in cacodylate-buffered 4% glutaraldehyde, post-fixed in 1% osmium tetroxide, dehydrated in ethanol, embedded in Epon-Araldite, sectioned at 100 nm, stained with uranyl acetate and lead citrate, and examined with a Philips EM201 electron microscope. Tissues from 5 control animals were processed in the same manner.

scholarly journals An electron stain for elastic fibers using orcein.

1977 ◽  
Vol 25 (4) ◽  
pp. 306-308 ◽  
Author(s):  
H Nakamura ◽  
C Kanai ◽  
V Mizuhira
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Muscle Cells ◽  
Uranyl Acetate ◽  
Elastic Fibers ◽  
Lead Citrate ◽  
Thin Sections ◽  
Electron Microscopes ◽  
Orcein Staining

Orcein was found to be useful as an electron-opaque stain for elastic fibers in epoxy-sections. Ultra-thin sections of aorta were treated with elastica stain containing 0.1-0.3% orcein and counterstained in uranyl acetate and lead citrate. Elastic fibers were densely and specifically demonstrated in the stroma and near smooth muscle cells. The result of orcein staining has a comparable appearance under both light and electron microscopes.

X-ray microanalytical and enzyme-cytochemical study on vesical malacoplakia

1978 ◽  
Vol 36 (2) ◽  
pp. 646-647
Author(s):  
Masami Hokano ◽  
Tsunao Oh-I ◽  
Yoshie Narita ◽  
Hiroshi Sassa ◽  
Saburo Suzuki
Keyword(s):  
Electron Microscopy ◽  
Electron Microscope ◽  
Osmium Tetroxide ◽  
Granulomatous Inflammation ◽  
Uranyl Acetate ◽  
The Other ◽  
Lead Citrate ◽  
Cytochemical Study ◽  
X Ray ◽  
Ultrathin Sections

Malacoplakia is a kind of granulomatous inflammation and characterized by the presence of calcium-stain positive granules (Michaelis-Gutmann bodies, hereinafter abbreviated as M-G bodies ) in the macrophages.In this report we want to say about the following articles:the ultrastruc tural findings in four cases of vesical malacoplakia;the ultrastructural morphogenesis of the M-G bodies;X-ray microanalytical studies which examine the change of the chemical component of M-G bodies, according to their developing stages; andacid phosphatase activity of lysosome within the malacoplakic macrophages.Biopsy materials taken from four cases with vesical malacoplakia were divided into 2 parts; one for a light microscopy and the other for an electron microscopy. The specimen for an electron microscopy was fixed in glutaraldehyde and osmium tetroxide, dehydrated in ethanol and then embedded in Epon 812. Ultrathin sections were doublestained with lead citrate and uranyl acetate and then subjected to routin TEM observation. For X-ray microanalysis was mounted an energy dispersive X-ray microanalyzer on a JEM-100 C type electron microscope. Ultrathin sections for microanalysis were cut 100-200nm thich and not stained.

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