scholarly journals Botanical microbiomes on the cheap: Inexpensive molecular fingerprinting methods to study plant‐associated communities of bacteria and fungi

2020 ◽  
Vol 8 (4) ◽  
Author(s):  
David Johnston‐Monje ◽  
Jessica Lopez Mejia
Keyword(s):  
Molecular Fingerprinting ◽  
Bacteria And Fungi ◽  
Fingerprinting Methods

Tracing the Origin of an Outbreak of Methicillin-ResistantStaphylococcus aureusInfections in a Portuguese Hospital by Molecular Fingerprinting Methods

1996 ◽  
Vol 2 (3) ◽  
pp. 319-329 ◽  
Author(s):  
I. SANTOS SANCHES ◽  
M. AIRES de SOUSA ◽  
L. CLETO ◽  
M. BAETA de CAMPOS ◽  
H. de LENCASTRE
Keyword(s):  
Molecular Fingerprinting ◽  
Fingerprinting Methods

Molecular fingerprinting methods for the discrimination between C. albicans and C. dubliniensis

Oral Diseases ◽  
2006 ◽  
Vol 12 (3) ◽  
pp. 242-253 ◽  
Author(s):  
KH Neppelenbroek ◽  
NH Campanha ◽  
DMP Spolidorio ◽  
LC Spolidorio ◽  
RS Seo ◽  
...  
Keyword(s):  
Molecular Fingerprinting ◽  
Fingerprinting Methods

scholarly journals Molecular Fingerprinting by PCR-Denaturing Gradient Gel Electrophoresis Reveals Differences in the Levels of Microbial Diversity for Musty-Earthy Tainted Corks

2009 ◽  
Vol 75 (7) ◽  
pp. 1922-1931 ◽  
Author(s):  
Chantal Prat ◽  
Olaya Ruiz-Rueda ◽  
Rosalia Trias ◽  
Enriqueta Anticó ◽  
Dimitra Capone ◽  
...  
Keyword(s):  
Gel Electrophoresis ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Small Contribution ◽  
Molecular Fingerprinting ◽  
Biological Degradation ◽  
Microbial Identification ◽  
Gradient Gel Electrophoresis ◽  
Bacteria And Fungi ◽  
Penicillium Variabile ◽  
Cork Stoppers

ABSTRACT The microbial community structure of cork with marked musty-earthy aromas was analyzed using denaturing gradient gel electrophoresis of amplified ribosomal DNA. Cork stoppers and discs were used for DNA extraction and were analyzed by using selective primers for bacteria and fungi. Stoppers clearly differed from discs harboring a different fungal community. Moreover, musty-earthy samples of both types were shown to have a specific microbiota. The fungi Penicillium glabrum and Neurospora spp. were present in all samples and were assumed to make only a small contribution to off-odor development. In contrast, Penicillium islandicum and Penicillium variabile were found almost exclusively in 2,4,6-trichloroanisole (TCA) tainted discs. Conversely, Rhodotorula minuta and Rhodotorula sloofiae were most common in cork stoppers, where only small amounts of TCA were detected. Alpha- and gammaproteobacteria were the most commonly found bacteria in either control or tainted cork stoppers. Specific Pseudomonas and Actinobacteria were detected in stoppers with low amounts of TCA and 2-methoxy-3,5-dimethylpyrazine. These results are discussed in terms of biological degradation of taint compounds by specific microorganisms. Reliable and straightforward microbial identification methods based on a molecular approach provided useful data to determine and evaluate the risk of taint formation in cork.

scholarly journals Monitoring of Rhizosphere Bacterial Communities in Soil with Sewage Sludge Addition Using Two Molecular Fingerprinting Methods: Do These Methods Give Similar Results?

2016 ◽  
Vol 62 (2) ◽  
pp. 52-61 ◽  
Author(s):  
Katarína Ondreičková ◽  
Alžbeta Žofajová ◽  
Michaela Piliarová ◽  
Jozef Gubiš ◽  
Martina Hudcovicová
Keyword(s):  
Sewage Sludge ◽  
Bacterial Community ◽  
Bacterial Communities ◽  
Intergenic Spacer ◽  
Diversity Indices ◽  
Maturity Stage ◽  
Molecular Fingerprinting ◽  
Rhizosphere Bacterial Communities ◽  
Fingerprinting Methods ◽  
Soil Additive

Abstract In this study, bacterial genetic diversity from the rhizosphere of barley and wheat were studied. The plants were sown in pots with aliquot amount of 15 t/ha concentration of soil additive derived from sewage sludge and agricultural by-products represented by wastes from grain mill industry and crushed corn cobs. The plants sown in pots without the addition of soil additive represented control samples. The rhizosphere samples were collected on two dates (plant flowering and maturity) and the composition of bacterial communities were detected using two molecular fingerprinting methods – automated ribosomal intergenic spacer analysis (ARISA) and terminal restriction fragment length polymorphism (T-RFLP). Microbial biomass expressed as the amount of metagenomics DNA was higher in soils with addition of soil additive, except during maturity stage in barley rhizosphere. Nevertheless, statistically significant differences between control and sludge samples were not detected in any case. Similarly, no changes were detected in the composition of bacterial community between control and sludge samples in barley and wheat rhizosphere by using cluster analysis. Only minor temporal changes in the composition of bacterial community between flowering and maturity periods were observed. These changes were related to the samples collected in the plant maturity stage. In this stage, plants were completely mature and their impact on the rhizosphere bacterial communities in the form of root exudates was limited. Statistically significant differences between ARISA and T-RFLP methods were detected in all measured values of diversity indices. Despite these differences, both methods gave results leading to similar conclusions.

scholarly journals The Structure and Diversity of Bacterial Communities in Differently Managed Soils Studied by Molecular Fingerprinting Methods

2018 ◽  
Vol 10 (4) ◽  
pp. 1095 ◽  
Author(s):  
Katarína Ondreičková ◽  
Michaela Piliarová ◽  
Rastislav Bušo ◽  
Roman Hašana ◽  
Ľudovít Schreiber ◽  
...  
Keyword(s):  
Bacterial Communities ◽  
Molecular Fingerprinting ◽  
Fingerprinting Methods
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