scholarly journals The Structure and Diversity of Bacterial Communities in Differently Managed Soils Studied by Molecular Fingerprinting Methods

2018 ◽  
Vol 10 (4) ◽  
pp. 1095 ◽  
Author(s):  
Katarína Ondreičková ◽  
Michaela Piliarová ◽  
Rastislav Bušo ◽  
Roman Hašana ◽  
Ľudovít Schreiber ◽  
...  
Keyword(s):  
Bacterial Communities ◽  
Molecular Fingerprinting ◽  
Fingerprinting Methods

scholarly journals Monitoring of Rhizosphere Bacterial Communities in Soil with Sewage Sludge Addition Using Two Molecular Fingerprinting Methods: Do These Methods Give Similar Results?

2016 ◽  
Vol 62 (2) ◽  
pp. 52-61 ◽  
Author(s):  
Katarína Ondreičková ◽  
Alžbeta Žofajová ◽  
Michaela Piliarová ◽  
Jozef Gubiš ◽  
Martina Hudcovicová
Keyword(s):  
Sewage Sludge ◽  
Bacterial Community ◽  
Bacterial Communities ◽  
Intergenic Spacer ◽  
Diversity Indices ◽  
Maturity Stage ◽  
Molecular Fingerprinting ◽  
Rhizosphere Bacterial Communities ◽  
Fingerprinting Methods ◽  
Soil Additive

Abstract In this study, bacterial genetic diversity from the rhizosphere of barley and wheat were studied. The plants were sown in pots with aliquot amount of 15 t/ha concentration of soil additive derived from sewage sludge and agricultural by-products represented by wastes from grain mill industry and crushed corn cobs. The plants sown in pots without the addition of soil additive represented control samples. The rhizosphere samples were collected on two dates (plant flowering and maturity) and the composition of bacterial communities were detected using two molecular fingerprinting methods – automated ribosomal intergenic spacer analysis (ARISA) and terminal restriction fragment length polymorphism (T-RFLP). Microbial biomass expressed as the amount of metagenomics DNA was higher in soils with addition of soil additive, except during maturity stage in barley rhizosphere. Nevertheless, statistically significant differences between control and sludge samples were not detected in any case. Similarly, no changes were detected in the composition of bacterial community between control and sludge samples in barley and wheat rhizosphere by using cluster analysis. Only minor temporal changes in the composition of bacterial community between flowering and maturity periods were observed. These changes were related to the samples collected in the plant maturity stage. In this stage, plants were completely mature and their impact on the rhizosphere bacterial communities in the form of root exudates was limited. Statistically significant differences between ARISA and T-RFLP methods were detected in all measured values of diversity indices. Despite these differences, both methods gave results leading to similar conclusions.

Tracing the Origin of an Outbreak of Methicillin-ResistantStaphylococcus aureusInfections in a Portuguese Hospital by Molecular Fingerprinting Methods

1996 ◽  
Vol 2 (3) ◽  
pp. 319-329 ◽  
Author(s):  
I. SANTOS SANCHES ◽  
M. AIRES de SOUSA ◽  
L. CLETO ◽  
M. BAETA de CAMPOS ◽  
H. de LENCASTRE
Keyword(s):  
Molecular Fingerprinting ◽  
Fingerprinting Methods

Molecular fingerprinting methods for the discrimination between C. albicans and C. dubliniensis

Oral Diseases ◽  
2006 ◽  
Vol 12 (3) ◽  
pp. 242-253 ◽  
Author(s):  
KH Neppelenbroek ◽  
NH Campanha ◽  
DMP Spolidorio ◽  
LC Spolidorio ◽  
RS Seo ◽  
...  
Keyword(s):  
Molecular Fingerprinting ◽  
Fingerprinting Methods

scholarly journals P467 Molecular fingerprinting techniques reveal distinct luminal and mucosa-associated bacterial communities in the human colon

2012 ◽  
Vol 6 ◽  
pp. S195
Author(s):  
A. Lavelle ◽  
G. Lennon ◽  
N. Docherty ◽  
J. Hyland ◽  
D. O'Donoghue ◽  
...  
Keyword(s):  
Bacterial Communities ◽  
Human Colon ◽  
Molecular Fingerprinting

scholarly journals Within- and between-Lake Variability in the Composition of Bacterioplankton Communities: Investigations Using Multiple Spatial Scales

2004 ◽  
Vol 70 (1) ◽  
pp. 214-223 ◽  
Author(s):  
Anthony C. Yannarell ◽  
Eric W. Triplett
Keyword(s):  
Organic Carbon ◽  
Dissolved Organic Carbon ◽  
Bacterial Communities ◽  
Spatial Scales ◽  
Bacterial Community Composition ◽  
Intergenic Spacer ◽  
Molecular Fingerprinting ◽  
Concentration Gradients ◽  
Lake Productivity ◽  
Organic Carbon Concentration

ABSTRACT This study examined the similarity of epilimnetic bacterial community composition (BCC) across several within- and among-lake spatial scales, and the environmental factors giving rise to similar bacterial communities in different lakes were also explored. Samples were collected from 13 northern and southern Wisconsin lakes representing gradients in lake size, productivity, dissolved organic carbon and humic acid contents, and pH. Hypotheses regarding patchy distribution of bacterial communities in lakes were tested by comparing samples collected from nearby (tens of meters) and distant (hundreds of meters) sampling sites in the same lake. BCC was characterized by using a molecular fingerprinting technique, automated ribosomal intergenic spacer analysis (ARISA). Overall, samples collected at the 10-m, 100-m, and between-lake scales differed by 13, 17, and 75%, respectively. Variation at these last two scales was significant. The development of within-lake variation in BCC appeared to depend on the isolation of water by lake shoreline features such as bays or narrow constrictions. ARISA profiles from northern lakes had fewer peaks and were less similar to each other than were those of the southern lakes, suggesting that regional features do not necessarily lead to the development of similar bacterial communities. Lakes at similar positions on productivity and dissolved organic carbon concentration gradients had similar bacterial communities, and bacterial diversity was positively correlated with lake productivity and water temperature. Factorial studies taking into account these gradients, as well as regional spatial scales, should provide much insight into the nature of aquatic bacterial biogeography.

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