scholarly journals Magnetized Carbon Nanotube Based Lateral Flow Immunoassay for Visual Detection of Complement Factor B

Molecules ◽  
2019 ◽  
Vol 24 (15) ◽  
pp. 2759 ◽  
Author(s):  
Yan Huang ◽  
Tingting Wu ◽  
Fang Wang ◽  
Kun Li ◽  
Lisheng Qian ◽  
...  
Keyword(s):  
Carbon Nanotube ◽  
Human Blood ◽  
Visual Detection ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Complement Factor ◽  
Factor B ◽  
Complement Factor B ◽  
Flow Test ◽  
Lateral Flow Test

The authors describe a magnetized carbon nanotube (MCNT) based lateral flow immunoassay (LFI) for visual detection of complement factor B (CFB) in blood. MCNT was prepared by decorating magnetic Fe3O4 nanoparticles on multi-walled CNT surface and used as a colored tag for LFI. Monoclonal antibody (mAb, Ab1) of CFB was covalently immobilized on the MCNT surface via diimide-activated conjugation between the carboxyl groups on the MCNT surface and amino groups of antibodies. Polyclonal antibody of CFB (Ab2) and the secondary antibody were used to prepare the lateral flow test strips. The assay involved: (1) the capture of CFB in blood with the mAb-functionalized MCNT; (2) magnetic separation of the formed CFB-mAb-MCNT and excess of mAb-MCNT from the blood with an external magnet; (3) lateral flow test to capture the CFB-mAb-MCNT complex on the test zone and the excess of mAb-MCNT on the control zone; (4) Recording the intensities of the produced the characteristic brown bands with a portable strip reader and quantitating the concentration of CFB. The proof-of-concept was demonstrated by testing CFB in the buffer, and the detection limit was 5 ng mL−1 under the optimized analytical parameters. CFB in 1 μL of human blood was detected successfully in 30 min with this LFI and the results had a high correlation with commercial ELISA kit. Thence, the MCNT-based LFI offers a rapid and low-cost tool for detecting CFB in human blood directly.

Rapid and visual detection of benzothiostrobin residue in strawberry using quantum dot-based lateral flow test strip

2019 ◽  
Vol 283 ◽  
pp. 222-229 ◽  
Author(s):  
JiaKai Wu ◽  
JingWei Ma ◽  
Hong Wang ◽  
DongMei Qin ◽  
Li An ◽  
...  
Keyword(s):  
Quantum Dot ◽  
Visual Detection ◽  
Test Strip ◽  
Lateral Flow ◽  
Flow Test ◽  
Lateral Flow Test

scholarly journals Lateral flow test for visual detection of silver(I) based on cytosine-Ag(I)-cytosine interaction in C-rich oligonucleotides

2017 ◽  
Vol 184 (11) ◽  
pp. 4243-4250 ◽  
Author(s):  
Zebin Guo ◽  
Yafeng Zheng ◽  
Hui Xu ◽  
Baodong Zheng ◽  
Wanwei Qiu ◽  
...  
Keyword(s):  
Visual Detection ◽  
Lateral Flow ◽  
Flow Test ◽  
Lateral Flow Test

scholarly journals Signal-Amplified Lateral Flow Test Strip for Visual Detection of Cu2+

PLoS ONE ◽  
2017 ◽  
Vol 12 (1) ◽  
pp. e0169345 ◽  
Author(s):  
Yulong Wang ◽  
Limin Wang ◽  
Juanjuan Xue ◽  
Jinbo Dong ◽  
Jia Cai ◽  
...  
Keyword(s):  
Visual Detection ◽  
Test Strip ◽  
Lateral Flow ◽  
Flow Test ◽  
Lateral Flow Test

scholarly journals Lateral flow test for visual detection of multiple MicroRNAs

2018 ◽  
Vol 264 ◽  
pp. 320-326 ◽  
Author(s):  
Wanli Zheng ◽  
Li Yao ◽  
Jun Teng ◽  
Chao Yan ◽  
Panzhu Qin ◽  
...  
Keyword(s):  
Visual Detection ◽  
Lateral Flow ◽  
Flow Test ◽  
Lateral Flow Test

scholarly journals Development of a rapid and visual detection method for Rickettsia rickettsii combining recombinase polymerase assay with lateral flow test

PLoS ONE ◽  
2018 ◽  
Vol 13 (11) ◽  
pp. e0207811 ◽  
Author(s):  
Yong Qi ◽  
Yinxiu Shao ◽  
Jixian Rao ◽  
Wanpeng Shen ◽  
Qiong Yin ◽  
...  
Keyword(s):  
Visual Detection ◽  
Detection Method ◽  
Lateral Flow ◽  
Rickettsia Rickettsii ◽  
Flow Test ◽  
Lateral Flow Test

Lateral flow test for meat authentication with visual detection

2019 ◽  
Vol 274 ◽  
pp. 803-807 ◽  
Author(s):  
Maria Magiati ◽  
Vasiliki M. Myridaki ◽  
Theodore K. Christopoulos ◽  
Despina P. Kalogianni
Keyword(s):  
Visual Detection ◽  
Lateral Flow ◽  
Flow Test ◽  
Lateral Flow Test

scholarly journals Enlargement of Gold Nanoparticles for Sensitive Immunochromatographic Diagnostics of Potato Brown Rot

Sensors ◽  
2019 ◽  
Vol 19 (1) ◽  
pp. 153 ◽  
Author(s):  
Shyatesa C. Razo ◽  
Natalia A. Panferova ◽  
Vasily G. Panferov ◽  
Irina V. Safenkova ◽  
Natalia V. Drenova ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Detection Limit ◽  
Brown Rot ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Test Strips ◽  
Flow Test ◽  
Test Zone ◽  
Lateral Flow Test ◽  
Potato Brown Rot

Lateral flow immunoassay (LFIA) is a convenient tool for rapid field-based control of various bacterial targets. However, for many applications, the detection limits obtained by LFIA are not sufficient. In this paper, we propose enlarging gold nanoparticles’ (GNPs) size to develop a sensitive lateral flow immunoassay to detect Ralstonia solanacearum. This bacterium is a quarantine organism that causes potato brown rot. We fabricated lateral flow test strips using gold nanoparticles (17.4 ± 1.0 nm) as a label and their conjugates with antibodies specific to R. solanacearum. We proposed a signal enhancement in the test strips’ test zone due to the tetrachloroauric (III) anion reduction on the GNP surface, and the increase in size of the gold nanoparticles on the test strips was approximately up to 100 nm, as confirmed by scanning electron microscopy. Overall, the gold enhancement approach decreased the detection limit of R. solanacearum by 33 times, to as low as 3 × 104 cells∙mL–1 in the potato tuber extract. The achieved detection limit allows the diagnosis of latent infection in potato tubers. The developed approach based on gold enhancement does not complicate analyses and requires only 3 min. The developed assay together with the sample preparation and gold enlargement requires 15 min. Thus, the developed approach is promising for the development of lateral flow test strips and their subsequent introduction into diagnostic practice.

scholarly journals Plasma protein expression profiles, cardiovascular disease, and religious struggles among South Asians in the MASALA study

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Long H. Ngo ◽  
M. Austin Argentieri ◽  
Simon T. Dillon ◽  
Blake Victor Kent ◽  
Alka M. Kanaya ◽  
...  
Keyword(s):  
Cardiovascular Disease ◽  
Plasma Protein ◽  
Expression Profiles ◽  
South Asians ◽  
Predictive Biomarkers ◽  
Information Criteria ◽  
Blood Protein ◽  
Complement Factor ◽  
Factor B ◽  
Complement Factor B

AbstractBlood protein concentrations are clinically useful, predictive biomarkers of cardiovascular disease (CVD). Despite a higher burden of CVD among U.S. South Asians, no CVD-related proteomics study has been conducted in this sub-population. The aim of this study is to investigate the associations between plasma protein levels and CVD incidence, and to assess the potential influence of religiosity/spirituality (R/S) on significant protein-CVD associations, in South Asians from the MASALA Study. We used a nested case–control design of 50 participants with incident CVD and 50 sex- and age-matched controls. Plasma samples were analyzed by SOMAscan for expression of 1305 proteins. Multivariable logistic regression models and model selection using Akaike Information Criteria were performed on the proteins and clinical covariates, with further effect modification analyses conducted to assess the influence of R/S measures on significant associations between proteins and incident CVD events. We identified 36 proteins that were significantly expressed differentially among CVD cases compared to matched controls. These proteins are involved in immune cell recruitment, atherosclerosis, endothelial cell differentiation, and vascularization. A final multivariable model found three proteins (Contactin-5 [CNTN5], Low affinity immunoglobulin gamma Fc region receptor II-a [FCGR2A], and Complement factor B [CFB]) associated with incident CVD after adjustment for diabetes (AUC = 0.82). Religious struggles that exacerbate the adverse impact of stressful life events, significantly modified the effect of Contactin-5 and Complement factor B on risk of CVD. Our research is this first assessment of the relationship between protein concentrations and risk of CVD in a South Asian sample. Further research is needed to understand patterns of proteomic profiles across diverse ethnic communities, and the influence of resources for resiliency on proteomic signatures and ultimately, risk of CVD.

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