Corrigendum: A Bioreducible Polymer for Efficient Delivery of Fas-Silencing siRNA into Stem Cell Spheroids and Enhanced Therapeutic Angiogenesis

2013 ◽  
Vol 52 (8) ◽  
pp. 2147-2147
Author(s):  
Min Suk Shim ◽  
Suk Ho Bhang ◽  
Kyunghwan Yoon ◽  
Kyunghee Choi ◽  
Younan Xia
Keyword(s):  
Stem Cell ◽  
Therapeutic Angiogenesis ◽  
Cell Spheroids ◽  
Efficient Delivery

scholarly journals Berichtigung: A Bioreducible Polymer for Efficient Delivery of Fas-Silencing siRNA into Stem Cell Spheroids and Enhanced Therapeutic Angiogenesis

2013 ◽  
Vol 125 (8) ◽  
pp. 2201-2201
Author(s):  
Min Suk Shim ◽  
Suk Ho Bhang ◽  
Kyunghwan Yoon ◽  
Kyunghee Choi ◽  
Younan Xia
Keyword(s):  
Stem Cell ◽  
Therapeutic Angiogenesis ◽  
Cell Spheroids ◽  
Efficient Delivery

scholarly journals A Bioreducible Polymer for Efficient Delivery of Fas-Silencing siRNA into Stem Cell Spheroids and Enhanced Therapeutic Angiogenesis

2012 ◽  
Vol 51 (47) ◽  
pp. 11899-11903 ◽  
Author(s):  
Min Suk Shim ◽  
Suk Ho Bhang ◽  
Kyunghwan Yoon ◽  
Kyunghee Choi ◽  
Younan Xia
Keyword(s):  
Stem Cell ◽  
Therapeutic Angiogenesis ◽  
Cell Spheroids ◽  
Efficient Delivery

scholarly journals A Bioreducible Polymer for Efficient Delivery of Fas-Silencing siRNA into Stem Cell Spheroids and Enhanced Therapeutic Angiogenesis

2012 ◽  
Vol 124 (47) ◽  
pp. 12069-12073 ◽  
Author(s):  
Min Suk Shim ◽  
Suk Ho Bhang ◽  
Kyunghwan Yoon ◽  
Kyunghee Choi ◽  
Younan Xia
Keyword(s):  
Stem Cell ◽  
Therapeutic Angiogenesis ◽  
Cell Spheroids ◽  
Efficient Delivery

scholarly journals Core–shell hydrogel microcapsules enable formation of human pluripotent stem cell spheroids and their cultivation in a stirred bioreactor

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Pouria Fattahi ◽  
Ali Rahimian ◽  
Michael Q. Slama ◽  
Kihak Gwon ◽  
Alan M. Gonzalez-Suarez ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Scale Up ◽  
Bioreactor Culture ◽  
Stirred Bioreactor ◽  
Cell Spheroids ◽  
Poly Ethylene ◽  
Aqueous Core ◽  
End Stage ◽  
Stirred Bioreactors

AbstractCellular therapies based on human pluripotent stem cells (hPSCs) offer considerable promise for treating numerous diseases including diabetes and end stage liver failure. Stem cell spheroids may be cultured in stirred bioreactors to scale up cell production to cell numbers relevant for use in humans. Despite significant progress in bioreactor culture of stem cells, areas for improvement remain. In this study, we demonstrate that microfluidic encapsulation of hPSCs and formation of spheroids. A co-axial droplet microfluidic device was used to fabricate 400 μm diameter capsules with a poly(ethylene glycol) hydrogel shell and an aqueous core. Spheroid formation was demonstrated for three hPSC lines to highlight broad utility of this encapsulation technology. In-capsule differentiation of stem cell spheroids into pancreatic β-cells in suspension culture was also demonstrated.

scholarly journals Rapid Formation of Stem Cell Spheroids Using Two-Dimensional MXene Particles

Processes ◽  
2021 ◽  
Vol 9 (6) ◽  
pp. 957
Author(s):  
JunHwee Jang ◽  
Eun-Jung Lee
Keyword(s):  
Stem Cell ◽  
Mass Production ◽  
Processing Time ◽  
Activity Levels ◽  
Two Dimensional ◽  
Spheroid Formation ◽  
Cell Spheroids ◽  
Tissue Healing ◽  
Rapid Formation ◽  
Cell Sources

Cell spheroids have been studied as a biomimic medicine for tissue healing using cell sources. Rapid cell spheroid production increases cell survival and activity as well as the efficiency of mass production by reducing processing time. In this study, two-dimensional MXene (Ti3C2) particles were used to form mesenchymal stem cell spheroids, and the optimal MXene concentration, spheroid-production times, and bioactivity levels of spheroid cells during this process were assessed. A MXene concentration range of 1 to 10 μg/mL induced spheroid formation within 6 h. The MXene-induced spheroids exhibited osteogenic-differentiation behavior, with the highest activity levels at a concentration of 5 μg/mL. We report a novel and effective method for the rapid formation of stem cell spheroids using MXene.

scholarly journals Spatially controlled glycocalyx engineering for growth factor patterning in embryoid bodies

2021 ◽  
Author(s):  
Matthew R. Naticchia ◽  
Logan K. Laubach ◽  
Daniel J. Honigfort ◽  
Sean C. Purcell ◽  
Kamil Godula
Keyword(s):  
Stem Cell ◽  
Growth Factor ◽  
Embryoid Bodies ◽  
Cell Spheroids ◽  
Tissue Models

Growth factor (GF) patterning in stem cell spheroids, such as embryoid bodies (EBs), has been sought to guide their differentiation and organization into functional 3D tissue models and organoids.

scholarly journals Size-controlled human adipose-derived stem cell spheroids hybridized with single-segmented nanofibers and their effect on viability and stem cell differentiation

2021 ◽  
Vol 25 (1) ◽  
Author(s):  
Jinkyu Lee ◽  
Sangmin Lee ◽  
Sung Min Kim ◽  
Heungsoo Shin
Keyword(s):  
Stem Cell ◽  
Cell Function ◽  
Average Length ◽  
Three Dimensional ◽  
Stem Cell Differentiation ◽  
Cell Spheroids ◽  
Significant Difference ◽  
Vitro Analysis ◽  
Physical And Chemical

Abstract Background Fabrication of three-dimensional stem cell spheroids have been studied to improve stem cell function, but the hypoxic core and limited penetration of nutrients and signaling cues to the interior of the spheroid were challenges. The incorporation of polymers such as silica and gelatin in spheroids resulted in relatively relaxed assembly of composite spheroids, and enhancing transport of nutrient and biological gas. However, because of the low surface area between cells and since the polymers were heterogeneously distributed throughout the spheroid, these polymers cannot increase the cell to extracellular matrix interactions needed to support differentiation. Methods We developed the stem cell spheroids that incorporate poly(ι-lactic acid) single-segmented fibers synthesized by electrospinning and physical and chemical fragmentation. The proper mixing ratio was 2000 cells/μg fibers (average length of the fibers was 50 μm - 100 μm). The SFs were coated with polydopamine to increase cell binding affinity and to synthesize various-sized spheroids. The function of spheroids was investigated by in vitro analysis depending on their sizes. For statistical analysis, Graphpad Prism 5 software (San Diego, CA, USA) was used to perform one-way analysis of variance ANOVA with Tukey’s honest significant difference test and a Student’s t-test (for two variables) (P < 0.05). Results Spheroids of different sizes were created by modulating the amount of cells and fibers (0.063 mm2–0.322 mm2). The fibers in the spheroid were homogenously distributed and increased cell viability, while cell-only spheroids showed a loss of DNA contents, internal degradation, and many apoptotic signals. Furthermore, we investigated stemness and various functions of various-sized fiber-incorporated spheroids. In conclusion, the spheroid with the largest size showed the greatest release of angiogenic factors (released VEGF: 0.111 ± 0.004 pg/ng DNA), while the smallest size showed greater effects of osteogenic differentiation (mineralized calcium: 18.099 ± 0.271 ng/ng DNA). Conclusion The spheroids incorporating polydopamine coated single-segmented fibers showed enhanced viability regardless of sizes and increased their functionality by regulating the size of spheroids which may be used for various tissue reconstruction and therapeutic applications.

scholarly journals Biologically produced silver chloride nanoparticles from B. megaterium modulate interleukin secretion by human adipose stem cell spheroids

2018 ◽  
Vol 70 (6) ◽  
pp. 1655-1669
Author(s):  
Letícia E. Charelli ◽  
Nathalia Müller ◽  
Karina R. Silva ◽  
Luís Maurício T. R. Lima ◽  
Celso Sant’Anna ◽  
...  
Keyword(s):  
Stem Cell ◽  
Silver Chloride ◽  
Adipose Stem Cell ◽  
Cell Spheroids ◽  
Human Adipose Stem Cell
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