scholarly journals Rapid Formation of Stem Cell Spheroids Using Two-Dimensional MXene Particles

Processes ◽  
2021 ◽  
Vol 9 (6) ◽  
pp. 957
Author(s):  
JunHwee Jang ◽  
Eun-Jung Lee
Keyword(s):  
Stem Cell ◽  
Mass Production ◽  
Processing Time ◽  
Activity Levels ◽  
Two Dimensional ◽  
Spheroid Formation ◽  
Cell Spheroids ◽  
Tissue Healing ◽  
Rapid Formation ◽  
Cell Sources

Cell spheroids have been studied as a biomimic medicine for tissue healing using cell sources. Rapid cell spheroid production increases cell survival and activity as well as the efficiency of mass production by reducing processing time. In this study, two-dimensional MXene (Ti3C2) particles were used to form mesenchymal stem cell spheroids, and the optimal MXene concentration, spheroid-production times, and bioactivity levels of spheroid cells during this process were assessed. A MXene concentration range of 1 to 10 μg/mL induced spheroid formation within 6 h. The MXene-induced spheroids exhibited osteogenic-differentiation behavior, with the highest activity levels at a concentration of 5 μg/mL. We report a novel and effective method for the rapid formation of stem cell spheroids using MXene.

Hydrogels with an embossed surface: An all-in-one platform for mass production and culture of human adipose-derived stem cell spheroids

Biomaterials ◽  
2019 ◽  
Vol 188 ◽  
pp. 198-212 ◽  
Author(s):  
Se-jeong Kim ◽  
Jaesung Park ◽  
Hayeon Byun ◽  
Young-Woo Park ◽  
Luke G. Major ◽  
...  
Keyword(s):  
Stem Cell ◽  
Mass Production ◽  
Cell Spheroids ◽  
Adipose Derived Stem Cell

scholarly journals Microfluidic fabrication of bioactive microgels for rapid formation and enhanced differentiation of stem cell spheroids

2016 ◽  
Vol 34 ◽  
pp. 125-132 ◽  
Author(s):  
Christian Siltanen ◽  
Maliheh Yaghoobi ◽  
Amranul Haque ◽  
Jungmok You ◽  
Jeremy Lowen ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Spheroids ◽  
Rapid Formation

scholarly journals Core–shell hydrogel microcapsules enable formation of human pluripotent stem cell spheroids and their cultivation in a stirred bioreactor

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Pouria Fattahi ◽  
Ali Rahimian ◽  
Michael Q. Slama ◽  
Kihak Gwon ◽  
Alan M. Gonzalez-Suarez ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Scale Up ◽  
Bioreactor Culture ◽  
Stirred Bioreactor ◽  
Cell Spheroids ◽  
Poly Ethylene ◽  
Aqueous Core ◽  
End Stage ◽  
Stirred Bioreactors

AbstractCellular therapies based on human pluripotent stem cells (hPSCs) offer considerable promise for treating numerous diseases including diabetes and end stage liver failure. Stem cell spheroids may be cultured in stirred bioreactors to scale up cell production to cell numbers relevant for use in humans. Despite significant progress in bioreactor culture of stem cells, areas for improvement remain. In this study, we demonstrate that microfluidic encapsulation of hPSCs and formation of spheroids. A co-axial droplet microfluidic device was used to fabricate 400 μm diameter capsules with a poly(ethylene glycol) hydrogel shell and an aqueous core. Spheroid formation was demonstrated for three hPSC lines to highlight broad utility of this encapsulation technology. In-capsule differentiation of stem cell spheroids into pancreatic β-cells in suspension culture was also demonstrated.

scholarly journals Emerging biogenesis technologies of extracellular vesicles for tissue regenerative therapeutics

2021 ◽  
Vol 12 ◽  
pp. 204173142110190
Author(s):  
Jung-Hwan Lee ◽  
Ji-Young Yoon ◽  
Jun Hee Lee ◽  
Hae-Hyoung Lee ◽  
Jonathan C Knowles ◽  
...  
Keyword(s):  
Extracellular Vesicles ◽  
Chemical Treatment ◽  
Mass Production ◽  
Disease Status ◽  
Clinical Translation ◽  
Technological Advances ◽  
Tissue Healing ◽  
Bio Imaging ◽  
Key Issues ◽  
Treatment Application

Extracellular vesicles (EVs), including exosomes, carry the genetic packages of RNA, DNA, and proteins and are heavily involved in cell-cell communications and intracellular signalings. Therefore, EVs are spotlighted as therapeutic mediators for the treatment of injured and dysfunctional tissues as well as biomarkers for the detection of disease status and progress. Several key issues in EVs, including payload content and bioactivity, targeting and bio-imaging ability, and mass-production, need to be improved to enable effective therapeutics and clinical translation. For this, significant efforts have been made recently, including genetic modification, biomolecular and chemical treatment, application of physical/mechanical cues, and 3D cultures. Here we communicate those recent technological advances made mainly in the biogenesis process of EVs or at post-collection stages, which ultimately aimed to improve the therapeutic efficacy in tissue healing and disease curing and the possibility of clinical translation. This communication will help tissue engineers and biomaterial scientists design and produce EVs optimally for tissue regenerative therapeutics.

scholarly journals Spatially controlled glycocalyx engineering for growth factor patterning in embryoid bodies

2021 ◽  
Author(s):  
Matthew R. Naticchia ◽  
Logan K. Laubach ◽  
Daniel J. Honigfort ◽  
Sean C. Purcell ◽  
Kamil Godula
Keyword(s):  
Stem Cell ◽  
Growth Factor ◽  
Embryoid Bodies ◽  
Cell Spheroids ◽  
Tissue Models

Growth factor (GF) patterning in stem cell spheroids, such as embryoid bodies (EBs), has been sought to guide their differentiation and organization into functional 3D tissue models and organoids.

scholarly journals Size-controlled human adipose-derived stem cell spheroids hybridized with single-segmented nanofibers and their effect on viability and stem cell differentiation

2021 ◽  
Vol 25 (1) ◽  
Author(s):  
Jinkyu Lee ◽  
Sangmin Lee ◽  
Sung Min Kim ◽  
Heungsoo Shin
Keyword(s):  
Stem Cell ◽  
Cell Function ◽  
Average Length ◽  
Three Dimensional ◽  
Stem Cell Differentiation ◽  
Cell Spheroids ◽  
Significant Difference ◽  
Vitro Analysis ◽  
Physical And Chemical

Abstract Background Fabrication of three-dimensional stem cell spheroids have been studied to improve stem cell function, but the hypoxic core and limited penetration of nutrients and signaling cues to the interior of the spheroid were challenges. The incorporation of polymers such as silica and gelatin in spheroids resulted in relatively relaxed assembly of composite spheroids, and enhancing transport of nutrient and biological gas. However, because of the low surface area between cells and since the polymers were heterogeneously distributed throughout the spheroid, these polymers cannot increase the cell to extracellular matrix interactions needed to support differentiation. Methods We developed the stem cell spheroids that incorporate poly(ι-lactic acid) single-segmented fibers synthesized by electrospinning and physical and chemical fragmentation. The proper mixing ratio was 2000 cells/μg fibers (average length of the fibers was 50 μm - 100 μm). The SFs were coated with polydopamine to increase cell binding affinity and to synthesize various-sized spheroids. The function of spheroids was investigated by in vitro analysis depending on their sizes. For statistical analysis, Graphpad Prism 5 software (San Diego, CA, USA) was used to perform one-way analysis of variance ANOVA with Tukey’s honest significant difference test and a Student’s t-test (for two variables) (P < 0.05). Results Spheroids of different sizes were created by modulating the amount of cells and fibers (0.063 mm2–0.322 mm2). The fibers in the spheroid were homogenously distributed and increased cell viability, while cell-only spheroids showed a loss of DNA contents, internal degradation, and many apoptotic signals. Furthermore, we investigated stemness and various functions of various-sized fiber-incorporated spheroids. In conclusion, the spheroid with the largest size showed the greatest release of angiogenic factors (released VEGF: 0.111 ± 0.004 pg/ng DNA), while the smallest size showed greater effects of osteogenic differentiation (mineralized calcium: 18.099 ± 0.271 ng/ng DNA). Conclusion The spheroids incorporating polydopamine coated single-segmented fibers showed enhanced viability regardless of sizes and increased their functionality by regulating the size of spheroids which may be used for various tissue reconstruction and therapeutic applications.

scholarly journals Measuring the bore straightness during reaming with sensoric tools

2020 ◽  
Vol 14 (4) ◽  
pp. 535-544
Author(s):  
Andreas Bretz ◽  
Eberhard Abele ◽  
Matthias Weigold
Keyword(s):  
Mass Production ◽  
Cost Efficiency ◽  
Machine Tools ◽  
Processing Time ◽  
Process Chain ◽  
Machining Processes ◽  
High Quality ◽  
Random Samples ◽  
Hydraulic Valves ◽  
Productive Time

Abstract Reaming plays a crucial role in production to meet the high quality requirements of precision bore machining. It is either directly responsible for the final component quality or influences subsequent processes such as honing. The narrow tolerances are usually monitored by measuring random samples in mass production due to cost efficiency. Having a closer look at an exemplary process chain for the production of hydraulic valves shows the possibility to adapt the honing parameters which reduces processing time and costs. However, the bore straightness after the reaming process has to be known. In this paper an approach is presented which allows to record the bore straightness within the productive time. For this purpose, a sensory reaming system is developed. It can be used without additional components in the machine tool and thus integrated into existing machining processes. Cutting tests show that the system is able to measure the bore straightness as good as sensing probes used in machine tools.

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