scholarly journals Spatially controlled glycocalyx engineering for growth factor patterning in embryoid bodies

2021 ◽  
Author(s):  
Matthew R. Naticchia ◽  
Logan K. Laubach ◽  
Daniel J. Honigfort ◽  
Sean C. Purcell ◽  
Kamil Godula
Keyword(s):  
Stem Cell ◽  
Growth Factor ◽  
Embryoid Bodies ◽  
Cell Spheroids ◽  
Tissue Models

Growth factor (GF) patterning in stem cell spheroids, such as embryoid bodies (EBs), has been sought to guide their differentiation and organization into functional 3D tissue models and organoids.

scholarly journals Effects of 6-bromoindirubin-3′-oxime on the maintenance of pluripotency of porcine embryonic germ cells in combination with stem cell factor, leukemia inhibitory factor and fibroblast growth factor

Reproduction ◽  
2010 ◽  
Vol 139 (6) ◽  
pp. 1039-1046 ◽  
Author(s):  
Jiang Wen ◽  
Juan Liu ◽  
Guangqi Song ◽  
Limei Liu ◽  
Bo Tang ◽  
...  
Keyword(s):  
Stem Cell ◽  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Germ Cells ◽  
Leukemia Inhibitory Factor ◽  
Stem Cell Factor ◽  
Glycogen Synthase ◽  
Embryoid Bodies ◽  
Inhibitory Factor ◽  
Fibroblast Growth

6-Bromoindirubin-3′-oxime (BIO), which is one of the glycogen synthase kinase 3 inhibitors and a key regulator of numerous signaling pathways, was reported to be capable of maintaining the pluripotency of human and mouse embryonic stem cells. Presently, it is unknown whether BIO can influence the derivation of porcine embryonic germ (EG) cells. In this study, porcine primordial germ cells (PGCs) were isolated from gonads of 24- and 28-day embryos, and were then treated with BIO either individually or in combination with other cytokines (stem cell factor (SCF), leukemia inhibitory factor (LIF), and fibroblast growth factor (FGF); abbreviated as ‘3F’), and the effects of the treatment on the proliferation ability of porcine PGCs at early stage were examined using 5-bromo-2-deoxyuridine (Brdu) immunostaining assay. After continuous culture, the effects on the efficiency of porcine undifferentiated EG cells in the third passage and differentiated EG cells from embryoid bodies were examined as well. The results obtained through the observation of the Brdu-labeled PGCs indicated that BIO in combination with 3F resulted in a significant increase in the mitosis index, and also indicated that the BIO in combination with 3F had a higher efficiency in promoting the formation of porcine EG colony derived from porcine day 24 PGCs than BIO used either individually or in combination with LIF. In addition, BIO in combination with 3F exhibited the apparent anti-differentiation activity by reversing the differentiated EG cells to the undifferentiated status. Our results demonstrate that BIO in combination with SCF, LIF, and FGF could significantly contribute to the establishment of a porcine EG cell colony and maintain the undifferentiated status.

scholarly journals Effects of Connective Tissue Growth Factor on the Cell Viability, Proliferation, Osteogenic Capacity and mRNA Expression of Stem Cell Spheroids

2021 ◽  
Vol 11 (14) ◽  
pp. 6572
Author(s):  
Abdullah Zaki Alnahash ◽  
Young-Min Song ◽  
Sae-Kyung Min ◽  
Hyun-Jin Lee ◽  
Min-Ji Kim ◽  
...  
Keyword(s):  
Stem Cell ◽  
Growth Factor ◽  
Connective Tissue ◽  
Cell Viability ◽  
Mrna Expression ◽  
Connective Tissue Growth Factor ◽  
Tissue Growth ◽  
Alizarin Red S ◽  
Alizarin Red ◽  
Cell Spheroids

Background: Connective tissue growth factor (CTGF) is a cellular communication network factor family protein involved in many cellular functions. The purpose of this study was to determine the effects of CTGF on the proliferation, osteogenic capacity, and mRNA expression of spheroids composed of gingiva-derived mesenchymal stem cells (GMSCs). Methods: CTGF was applied at final concentrations of 0, 25, 50, 100, and 200 ng/mL. Qualitative cell viability was determined using Live/Dead kit assay. Metabolic viability was determined with a colorimetric assay kit. Osteogenic activity was analyzed with alkaline phosphatase activity and Alizarin Red S staining. Quantitative polymerase chain reaction (qPCR) was used to assess the expression levels of RUNX2, BSP, OCN, and COL1A1. Results: In general, there was no significant difference in cell viability between the groups on Days 1, 4, and 7. Addition of CTGF produced an increase in Alizarin Red S staining. qPCR results demonstrated that the mRNA expression levels of RUNX2, BSP, OCN, and COL1A1 were significantly increased with the addition of CTGF. Conclusions: Based on these findings, we conclude that CTGF can be applied for increased osteogenic differentiation of stem cell spheroids.

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