Shaping Amorphous Calcium Carbonate Films into 2D Model Substrates for Bone Cell Culture

2006 ◽  
Vol 45 (11) ◽  
pp. 1762-1767 ◽  
Author(s):  
Daniela C. Popescu ◽  
Ellen N. M. van Leeuwen ◽  
Nicholas A. A. Rossi ◽  
Simon J. Holder ◽  
John A. Jansen ◽  
...  
Keyword(s):  
Cell Culture ◽  
Calcium Carbonate ◽  
Bone Cell ◽  
Amorphous Calcium Carbonate ◽  
Bone Cell Culture ◽  
2D Model

Shaping Amorphous Calcium Carbonate Films into 2D Model Substrates for Bone Cell Culture

2006 ◽  
Vol 118 (11) ◽  
pp. 1794-1799 ◽  
Author(s):  
Daniela C. Popescu ◽  
Ellen N. M. van Leeuwen ◽  
Nicholas A. A. Rossi ◽  
Simon J. Holder ◽  
John A. Jansen ◽  
...  
Keyword(s):  
Cell Culture ◽  
Calcium Carbonate ◽  
Bone Cell ◽  
Amorphous Calcium Carbonate ◽  
Bone Cell Culture ◽  
2D Model

A novel modular device for 3-D bone cell culture and non-destructive cell analysis

2010 ◽  
Vol 6 (9) ◽  
pp. 3798-3807 ◽  
Author(s):  
Friederike Kunz ◽  
Claudia Bergemann ◽  
Ernst-Dieter Klinkenberg ◽  
Arne Weidmann ◽  
Regina Lange ◽  
...  
Keyword(s):  
Cell Culture ◽  
Bone Cell ◽  
Cell Analysis ◽  
Non Destructive ◽  
Bone Cell Culture

scholarly journals THE EFFECT OF LOW LEVEL LASER IRRADIATION ON BONE CELL CULTURE

LASER THERAPY ◽  
1998 ◽  
Vol 10 (2) ◽  
pp. 55-57 ◽  
Author(s):  
EJ Luger ◽  
Y Wollman ◽  
S Rochkind ◽  
S Dekel ◽  
GE Ouaknine ◽  
...  
Keyword(s):  
Cell Culture ◽  
Laser Irradiation ◽  
Bone Cell ◽  
Low Level ◽  
Low Level Laser ◽  
Level Laser ◽  
Low Level Laser Irradiation ◽  
Bone Cell Culture

Article Commentary: Large Bony Defects: Can Bone Cell Culture Fill the Gap?

1994 ◽  
Vol 3 (4) ◽  
pp. 351-353 ◽  
Author(s):  
P.C. Nolan ◽  
D.P. Hankey ◽  
R.A.B. Mollan ◽  
D.J. Wilson
Keyword(s):  
Cell Culture ◽  
Bone Cell ◽  
Bone Cell Culture

scholarly journals The Effect of Chinese Medicine on Bone Cell Activities

2002 ◽  
Vol 30 (02n03) ◽  
pp. 271-285 ◽  
Author(s):  
Chun-Yu Lin ◽  
Jui-Sheng Sun ◽  
Shiow-Yunn Sheu ◽  
Feng-Huei Lin ◽  
Yng-Jiin Wang ◽  
...  
Keyword(s):  
Cell Culture ◽  
Beneficial Effect ◽  
Bone Cells ◽  
Colorimetric Assay ◽  
Cell Metabolism ◽  
Cell Activity ◽  
Bone Cell ◽  
Chinese Medicines ◽  
Bone Cell Culture

In this experiment, we investigate the biochemical effects of traditional Chinese medicines via an in vitro bone cell culture. Ten different Chinese medicines were used in this study. The rat osteoblast-osteoclast co-culture system was used as the experimental model. After the cells grew to 80% confluence, various tested materials were added. The mitochondria activity of the bone cells after exposure to various preparations of Chinese medicines was determined by colorimetric assay. Biochemical markers such as protein content, lactate dehydrogenase (LDH), alkaline phosphatase (ALP), and acid phosphatase (ACP) titer were analyzed to evaluate the bone cell activity. When cultured with various Chinese medicines for 24 hours, only four of these ten Chinese medicines had potential beneficial effects on the bone cell culture; and only Drynaria fortunei (Kunze) J. Sm. had a universal beneficial effect on bone cell metabolism. The major beneficial effect of Drynaria fortunei (Kunze) J. Sm. on bone cells is probably mediated by the induction of apoptosis of the osteoclast cell population. Continued study of alterations in gene expression of bone cells caused by Chinese medicines will improve our understanding of bone cell responses to various pharmacological interventions.

scholarly journals Cellular considerations for optimizing bone cell culture and remodeling in a lab-on-a-chip platform

BioTechniques ◽  
2020 ◽  
Vol 68 (5) ◽  
pp. 263-269 ◽  
Author(s):  
Sharon L Truesdell ◽  
Estee L George ◽  
Marnie M Saunders
Keyword(s):  
Cell Culture ◽  
Bone Formation ◽  
Bone Remodeling ◽  
Functional Activity ◽  
Bone Cells ◽  
Lab On A Chip ◽  
Bone Cell ◽  
Cell Seeding ◽  
Bone Cell Culture

Our lab has developed a lab-on-a-chip platform for bone remodeling that enables long-term culturing of bone cells out to 7 weeks and serves as a foundation toward a multicellular organ-on-a-chip system. Here, we optimized culturing protocols for osteoblasts, osteoclasts and osteocytes within the lab-on-a-chip and performed functional activity assays for quantifying bone formation and resorption. We analyzed cell seeding densities, feeding schedules and time in culture as a basis for optimizing culturing protocols. Further, we addressed concerns of sterility, cytotoxicity and leakage during the extended culture period within the polydimethylsiloxane chip. This system provides a method for quantifying the soluble effects of mechanically stimulated osteocytes on bone remodeling (formation/resorption).

scholarly journals Ki-Energy (Life-Energy) Stimulates Osteoblastic Cells and Inhibits the Formation of Osteoclast-Like Cells in Bone Cell Culture Models

2007 ◽  
Vol 4 (2) ◽  
pp. 225-232 ◽  
Author(s):  
S. Tsuyoshi Ohnishi ◽  
Kozo Nishino ◽  
Satoshi Uchiyama ◽  
Tomoko Ohnishi ◽  
Masayoshi Yamaguchi
Keyword(s):  
Cell Culture ◽  
Bone Mineralization ◽  
Bone Cell ◽  
Rt Pcr ◽  
Culture Models ◽  
Culture Stage ◽  
The Difference ◽  
And Gender ◽  
Cell Culture Models ◽  
Bone Cell Culture

Some practitioners of the Nishino Breathing Method (NBM) were found to have a higher bone density than the average values of age- and gender-matched non-practitioners. Using bone cell culture models, we investigated a possible mechanism behind this observation. For the study of bone mineralization, we performed the following two experiments using cultured osteoblastic MC3T3-E1 cells: (i) Kozo Nishino, a Japanese Ki expert, sent Ki-energy to the cells once for 5 or 10 min after they were seeded in culture dishes in the presence of 10% fetal bovine serum (FBS). They were incubated for 72 h and the cells were counted. The number in the dish with 10-min Ki-exposure was significantly greater than that in the control (P< 0.01 withn= 8). We performed a reverse transcription-polymerase chain reaction (RT–PCR) study using these cells, but the mRNA expressions did not change significantly. (ii) After cells were incubated for 72 h without Ki-exposure (in the presence of FBS), they were further cultured for 48 h (in the absence of FBS) to promote differentiation. At the beginning of the second culture stage, Ki was applied once for 10 min. After 48 h, RT–PCR was performed. The mRNA expressions which are related to bone mineralization, such as Runx2, α1(I) collagen, alkaline phosphatase and osteocalcin, increased significantly (P< 0.05 andn= 4 for all). For the bone resorption study, we used mouse marrow cultures, which can form osteoclast-like cells in the presence of (1–34) parathyroid hormone (PTH), and stimulate resorption. We exposed these cells to Ki-energy twice for the duration of 5 or 10 min on day 0 and day 4. On day 7, the cells were counted. The number of osteoclast-like cells in dishes with Ki exposure was significantly smaller than those in control dishes (P< 0.05 withn= 5). The difference between 5-min exposure and 10-min exposure was not statistically significant. All of our data suggest that the Ki-effect on osteoporosis should be further explored.

scholarly journals Biomimetic 3D Printing of Hierarchical and Interconnected Porous Hydroxyapatite Structures with High Mechanical Strength for Bone Cell Culture

2018 ◽  
Vol 21 (1) ◽  
pp. 1800678 ◽  
Author(s):  
Xiaolei Song ◽  
Halil Tetik ◽  
Thitikan Jirakittsonthon ◽  
Pedram Parandoush ◽  
Guang Yang ◽  
...  
Keyword(s):  
Cell Culture ◽  
3D Printing ◽  
Mechanical Strength ◽  
Bone Cell ◽  
High Mechanical Strength ◽  
Porous Hydroxyapatite ◽  
Bone Cell Culture
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