scholarly journals Comparative effects of parenteral antimalarials in Swiss albino mice after chronic exposure to Plasmodium berghei

2018 ◽  
Vol 1 (3) ◽  
pp. 235-241 ◽  
Author(s):  
Sylvester Aghahowa ◽  
Kenka Okolocha
Keyword(s):  
Plasmodium Berghei ◽  
Chronic Exposure ◽  
Swiss Albino Mice ◽  
Albino Mice

scholarly journals Evaluation of Antiplasmodial Activity of Hydroalcoholic Crude Extract and Solvent Fractions of Zehneria scabra Roots Against Plasmodium berghei in Swiss Albino Mice

2021 ◽  
Vol Volume 14 ◽  
pp. 2583-2596
Author(s):  
Dejen Nureye ◽  
Eyob Tekalign ◽  
Nebeyi Fisseha ◽  
Tarekegn Tesfaye ◽  
Workineh Woldeselassie Hammeso
Keyword(s):  
Crude Extract ◽  
Plasmodium Berghei ◽  
Antiplasmodial Activity ◽  
Swiss Albino Mice ◽  
Albino Mice

scholarly journals Hematological and Histopathological Profiles of Swiss Albino Mice Infected with Plasmodium berghei upon Treatment with Cooked Tetracarpidium conophorum Seed Ethanol Extract

2021 ◽  
pp. 22-37
Author(s):  
Dada Ebenezer ◽  
Oladipo Faith Temiloluwa
Keyword(s):  
Body Weight ◽  
Plasmodium Berghei ◽  
Hematological Parameters ◽  
Haemoglobin Concentration ◽  
Ethanol Extract ◽  
Seed Extract ◽  
Mean Corpuscular Hemoglobin ◽  
Swiss Albino Mice ◽  
Albino Mice ◽  
Group 5

Aim: This study was conducted to verify the hematological and histopathological outcome of cooked ethanol seed extract of Tetracarpidium conophorum in swiss albino mice infected with Plasmodium berghei (NK65). Study Design: Descriptive study. Methodology: Standard methods were conducted to determine the phyto-chemical profile of cooked T. conophorum seed ethanol extract, hematological, histopathological indices and biochemical assay. Mice were assembled into five groups (1, 2, 3, 4 and 5) of seven each. The mice in group 4 were treated with a customary antimalarial drug (chloroquine as positive control) at a dose of 5 mg/kg body weight, while mice in groups 3, 2 and 1 was administered with increasing dosages (200, 400, 800 mg/kg body weight) of seed extracts for four consecutive days respectively. Results: The qualitative phytochemical screening of T. conophorum seed extract revealed the presence of bioactive components such as saponin, tannins, flavonoid, terpenoid. Saponin (16.82 mg/100 g) was the highest occurring phytochemical followed by terpenoid (10.39 mg/100 g), glycoside (5.9 mg/100 g), tannin (2.8 mg/100 g) and flavonoid (1.17 mg/100 g), while steroid only had a trace presence. Hematological parameters revealed mice in all groups do not possess significantly different mean corpuscular haemoglobin concentration (MCHC), mean corpuscular hemoglobin (MCH) and mean corpuscular volume (MCV). Mice in group 5 (infected and not treated) demonstrated lowest values of hemoglobin, platelet, and packed cell volume with a significantly lower % eosin. There was considerable decrease in the levels of alanine transaminase and aspartate transaminase in group 5 compared to mice in groups 1, 2, and 3. Conclusion: Ameliorative effects of seed extract were observed on the liver and kidney of mice at dose levels (200 mg/kg) used, but the 400 mg/kg restored the kidney but had adverse effect on the liver. This study therefore shows that cooked T. conophorum is generally less effective and could not be employed for treatment of malaria.

scholarly journals Antiplasmodial and haematological effects of Senna occidentalis leaf ethanolic extracts on Swiss albino mice infected with Plasmodium berghei

2021 ◽  
Vol 12 (2) ◽  
pp. 122-128
Author(s):  
S.M. Babangida ◽  
G.J. Sow ◽  
D.M. Shehu
Keyword(s):  
Plasmodium Berghei ◽  
Blood Cells ◽  
Leaf Extract ◽  
White Blood Cells ◽  
Control Group ◽  
Phytochemical Analysis ◽  
Leaf Extracts ◽  
Swiss Albino Mice ◽  
Ethanolic Extracts ◽  
Albino Mice

A research was conducted to investigate the haematological effects of ethanolic leaf extracts of Senna occidentalis on Swiss albino mice infected with 0.2 ml of Plasmodium berghei infected blood. Fifteen (15) mice weighing between 140-260g were assigned into five study groups of three mice each. The first group is treated with 0.2 mL of normal saline (drug free control). Group 2, 3, 4 were treated with 100, 200, and 400mg/kg of theethanolic leaf extract respectively while group 5 received 10mg/kg of chloroquine phosphate. All doses were administered orally. The results obtained were analyzed using Analysis of Variance with Duncan’s Multiple Range Test to separate the means. The result of the preliminary phytochemical analysis revealed the presence of alkaloids, saponins, cardiac glycosides, diterpenoids, flavonoids, steroids, tannins, Triterpenoids, carbohydrates and proteins. The level of parasite suppression ranges from 35% to 75% and the activity increased with increase in concentration of the extracts (dose dependent). The extracts were found to increase the level of some haematological parameters such as Red Blood Cells, White Blood Cells and Haemoglobin. The effect is concentration dependent, increases with increase in concentration. Thus, the anti plasmodial efficacy of the leaf extract of S. occidentalis on P. berghei is confirmed. It is recommended that , 400 mg/kg leaf ethanolic extracts of S. occidentalis couldbe use in the treatment of malarial fever.

scholarly journals Phytochemical Analysis and Antiplasmodial (curative) Activities of Methanolic Leaf Extract of Morinda lucida (Ewe Oruwo) in Male Swiss Mice Infected with Plasmodium berghei NK65

2019 ◽  
pp. 1-13
Author(s):  
Momoh Johnson Oshiobugie ◽  
Damazio Olanrewaju Anthony ◽  
Ajetunmobi Asibiallau Oladipupo ◽  
Babalola Adenike Omosalewa ◽  
Adekunle Oluwasegun Michael ◽  
...  
Keyword(s):  
Body Weight ◽  
Vitamin A ◽  
Plasmodium Berghei ◽  
Leaf Extract ◽  
Liver Homogenate ◽  
Haematological Parameters ◽  
Swiss Albino Mice ◽  
Swiss Mice ◽  
Albino Mice ◽  
Group B

Aim: Medicinal plants have been used for the treatment of many infections and diseases including malaria. The study was conducted to determine the effect of in vivo anti-plasmodial and antioxidant properties of the methanolic leaf extract of Morinda lucida in male Swiss albino mice infected with Plasmodium Berghei NK65. Study Design and Methodology: Phytochemical, GC-MS and AAS analyses were determined in the plant. Swiss albino mice were inoculated intraperitoneally with Plasmodium berghei NK65. Thirty-five (35) mice were grouped into seven groups, five per group. Group A were not infected with P.berghei NK65. Group B, C and D served as the negative and positive control groups while Group E, F and G mice were treated with 400, 600 and 800 mg/kg body weight of methanolic leaf extract of M. lucida. Haematological parameters were determined in the whole blood using BC-3200 Auto Hematology Analyzer. TP, MDA, CAT, SOD % inhibition, SOD unit and vitamin A were all determined in the liver homogenate using standard procedures. Results: The GC-MS result of the M. lucida shows the presence of five bioactive compounds. It was also observed that the plant contains the following minerals: iron, magnesium, potassium, phosphorus and copper. Acute toxicity shows that the LD50 >000mg/Kg b.wt. The extract caused 30.96%, 32.93% and 67.23% reduction in parasitemia at 400, 600 and 800 mg/kg body weight respectively while chloroquine exerted 96.53% and artesunate exerted 92.03% reduction at 10 mg/kg body weight respectively. The Haematological parameters showed that the plant extract is not haematotoxic since it significantly (P<0.05) reduced WBC count, and increase RBC, HGB, and HCT values in the treated mice compared to the infected untreated mice. This study shows that the mean lipid peroxidation (MDA) level was significantly decreased in the malaria treated mice (group C, D, E, F and G) compared to the untreated mice (group B). There was also a significant increase in the total protein, catalase, SOD % inhibition, SOD unit and Vitamin A levels in the liver homogenate of animals treated with chloroquine, artesunate and extract of M. lucida compared to the untreated mice. Conclusions: The study shows that Morinda lucida possess antiplasmodial activity in male Swiss mice infected with Plasmodium berghei NK 65.

Sign in / Sign up

Export Citation Format

Share Document