scholarly journals Blue-conversion of Organic Dyes Produces Artifacts in Multicolor Fluorescence Imaging

2021 ◽  
Author(s):  
Do-Hyeon Kim ◽  
Yeonho Chang ◽  
Soyeon Park ◽  
Min Gyu Jeong ◽  
Yonghoon Kwon ◽  
...  
Keyword(s):  
Fluorescence Imaging ◽  
Organic Dyes ◽  
Image Artifacts ◽  
Multicolor Fluorescence

Multicolor fluorescence imaging is a powerful tool visualizing the spatiotemporal relationship among biomolecules. Here, we report that commonly employed organic dyes exhibit blue-conversion phenomenon, which can produce severe multicolor image artifacts...

Semiconductor Quantum Dots for Multicolor Fluorescence Imaging and Spectroscopy of Single Cancer Cells

2003 ◽  
Vol 773 ◽  
Author(s):  
Xiaohu Gao ◽  
Shuming Nie ◽  
Wallace H. Coulter
Keyword(s):  
Quantum Dots ◽  
Cancer Cells ◽  
Fluorescence Imaging ◽  
Organic Dyes ◽  
Fluorescent Proteins ◽  
Single Cells ◽  
Quantitative Imaging ◽  
Semiconductor Quantum Dots ◽  
Single Cancer ◽  
Imaging And Spectroscopy

AbstractLuminescent quantum dots (QDs) are emerging as a new class of biological labels with unique properties and applications that are not available from traditional organic dyes and fluorescent proteins. Here we report new developments in using semiconductor quantum dots for quantitative imaging and spectroscopy of single cancer cells. We show that both live and fixed cells can be labeled with multicolor QDs, and that single cells can be analyzed by fluorescence imaging and wavelength-resolved spectroscopy. These results raise new possibilities in cancer imaging, molecular profiling, and disease staging.

Multicolor fluorescence imaging of whole-mount Drosophila testes for studying spermatogenesis

2013 ◽  
Vol 436 (1) ◽  
pp. 55-64 ◽  
Author(s):  
Mikhail V. Kibanov ◽  
Alexei A. Kotov ◽  
Ludmila V. Olenina
Keyword(s):  
Fluorescence Imaging ◽  
Whole Mount ◽  
Multicolor Fluorescence

scholarly journals Multicolor Fluorescence Imaging of Traumatic Brain Injury in a Cryolesion Mouse Model

2012 ◽  
Vol 3 (7) ◽  
pp. 530-537 ◽  
Author(s):  
Bryan A. Smith ◽  
Bang-Wen Xie ◽  
Ermond R. van Beek ◽  
Ivo Que ◽  
Vicky Blankevoort ◽  
...  
Keyword(s):  
Traumatic Brain Injury ◽  
Brain Injury ◽  
Mouse Model ◽  
Fluorescence Imaging ◽  
Multicolor Fluorescence

Design of AIEgens for Near-Infrared IIb Imaging Through Structural Modulation at Molecular and Morphological Levels

2019 ◽  
Author(s):  
yuanyuan li ◽  
Zhaochong Cai ◽  
shunjie liu ◽  
Haoke Zhang ◽  
sherman Wong ◽  
...  
Keyword(s):  
Blood Vessels ◽  
Fluorescence Imaging ◽  
Near Infrared ◽  
Molecular Design ◽  
Organic Dyes ◽  
Tissue Imaging ◽  
Deep Penetration ◽  
Structural Modulation ◽  
Organic Fluorophore

<p>Fluorescence imaging in near-infrared IIb (NIR-IIb, 1500-1700 nm) spectrum holds a considerable promise for tissue imaging with deep penetration and high spatial resolution owing to the minimized autofluorescence and suppressed photon scattering. While few inorganic NIR-IIb fluorescent probes have been reported, their organic counterparts are still underdeveloped, possibly due to the lack of efficient materials with long emission wavelength. Herein, we propose a new molecular design philosophy to develop organic NIR-IIb fluorophores with high quantum yield (QY) by manipulation of the effects of twisted intramolecular charge transfer and aggregation-induced emission at the molecular and morphological levels. A pure organic fluorescent dye emitting up to 1600 nm with a QY of 14.2% in the NIR-II region (1000-1600 nm) is developed. For the first time, NIR-IIb fluorescence imaging of blood vessels and deeply-located intestinal tract of live mice based on organic dyes is achieved. The results show that organic fluorophore performs superb imaging ability in both superficial blood vessels and internal organs with high resolution and enhanced signal-to-background ratio in NIR-IIb region. We hope this groundbreakingly study will inspire further research on the evolution of pure organic NIR-IIb probes for in vivo imaging.</p>

Multicolor Fluorescence Imaging of Leaves-A Useful Tool for Visualizing Systemic Viral Infections in Plants†

2008 ◽  
Vol 84 (5) ◽  
pp. 1048-1060 ◽  
Author(s):  
Mónica Pineda ◽  
László Gáspár ◽  
Fermín Morales ◽  
Zoltán Szigeti ◽  
Matilde Barón
Keyword(s):  
Fluorescence Imaging ◽  
Viral Infections ◽  
Multicolor Fluorescence

Multicolor fluorescence imaging for early detection of the hypersensitive reaction to tobacco mosaic virus

2007 ◽  
Vol 164 (3) ◽  
pp. 253-262 ◽  
Author(s):  
Laury Chaerle ◽  
Sándor Lenk ◽  
Dik Hagenbeek ◽  
Claus Buschmann ◽  
Dominique Van Der Straeten
Keyword(s):  
Early Detection ◽  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Fluorescence Imaging ◽  
Hypersensitive Reaction ◽  
Multicolor Fluorescence

Multicolor fluorescence imaging during nodal dissections as a means to decrease surgery associated morbidity

2017 ◽  
Vol 16 (6) ◽  
pp. e2288
Author(s):  
P. Meershoek ◽  
G. Kleinjan ◽  
E. Wit ◽  
M. Van Oosterom ◽  
A. Mottrie ◽  
...  
Keyword(s):  
Fluorescence Imaging ◽  
Multicolor Fluorescence

Design of AIEgens for Near-Infrared IIb Imaging Through Structural Modulation at Molecular and Morphological Levels

2019 ◽  
Author(s):  
yuanyuan li ◽  
Zhaochong Cai ◽  
shunjie liu ◽  
Haoke Zhang ◽  
sherman Wong ◽  
...  
Keyword(s):  
Blood Vessels ◽  
Fluorescence Imaging ◽  
Near Infrared ◽  
Molecular Design ◽  
Organic Dyes ◽  
Tissue Imaging ◽  
Deep Penetration ◽  
Structural Modulation ◽  
Organic Fluorophore

<p>Fluorescence imaging in near-infrared IIb (NIR-IIb, 1500-1700 nm) spectrum holds a considerable promise for tissue imaging with deep penetration and high spatial resolution owing to the minimized autofluorescence and suppressed photon scattering. While few inorganic NIR-IIb fluorescent probes have been reported, their organic counterparts are still underdeveloped, possibly due to the lack of efficient materials with long emission wavelength. Herein, we propose a new molecular design philosophy to develop organic NIR-IIb fluorophores with high quantum yield (QY) by manipulation of the effects of twisted intramolecular charge transfer and aggregation-induced emission at the molecular and morphological levels. A pure organic fluorescent dye emitting up to 1600 nm with a QY of 14.2% in the NIR-II region (1000-1600 nm) is developed. For the first time, NIR-IIb fluorescence imaging of blood vessels and deeply-located intestinal tract of live mice based on organic dyes is achieved. The results show that organic fluorophore performs superb imaging ability in both superficial blood vessels and internal organs with high resolution and enhanced signal-to-background ratio in NIR-IIb region. We hope this groundbreakingly study will inspire further research on the evolution of pure organic NIR-IIb probes for in vivo imaging.</p>

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