scholarly journals 3D Printed Bone Scaffolds: Delivering Proangiogenic Factors from 3D‐Printed Polycaprolactone Scaffolds for Vascularized Bone Regeneration (Adv. Healthcare Mater. 23/2020)

2020 ◽  
Vol 9 (23) ◽  
pp. 2070083 ◽  
Author(s):  
Haoming Liu ◽  
Yingying Du ◽  
Gaojie Yang ◽  
Xixi Hu ◽  
Lin Wang ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Bone Scaffolds ◽  
3D Printed ◽  
Vascularized Bone

scholarly journals 3D Printed Wesselsite Nanosheets Functionalized Scaffold Facilitates NIR‐II Photothermal Therapy and Vascularized Bone Regeneration

2021 ◽  
pp. 2100894
Author(s):  
Chen Yang ◽  
Hongshi Ma ◽  
Zhiyong Wang ◽  
Muhammad Rizwan Younis ◽  
Chunyang Liu ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Photothermal Therapy ◽  
3D Printed ◽  
Vascularized Bone

3D-printed scaffolds with synergistic effect of hollow-pipe structure and bioactive ions for vascularized bone regeneration

Biomaterials ◽  
2017 ◽  
Vol 135 ◽  
pp. 85-95 ◽  
Author(s):  
Wenjie Zhang ◽  
Chun Feng ◽  
Guangzheng Yang ◽  
Guanglong Li ◽  
Xun Ding ◽  
...  
Keyword(s):  
Synergistic Effect ◽  
Bone Regeneration ◽  
3D Printed ◽  
Vascularized Bone

scholarly journals Vascularized Bone Regeneration Accelerated by 3D-Printed Nanosilicate-Functionalized Polycaprolactone Scaffold

2021 ◽  
Author(s):  
Xiongcheng Xu ◽  
Long Xiao ◽  
Yanmei Xu ◽  
Jin Zhuo ◽  
Xue Yang ◽  
...  
Keyword(s):  
3D Printing ◽  
Bone Regeneration ◽  
Bone Repair ◽  
Cytokine Secretion ◽  
Printing Technology ◽  
3D Printing Technology ◽  
3D Printed ◽  
Vascularized Bone

Abstract Critical oral-maxillofacial bone defects, damaged by trauma and tumors, not only affect the physiological functions and mental health of patients but are also highly challenging to reconstruct. Personalized biomaterials customized by 3D printing technology have the potential to match oral-maxillofacial bone repair and regeneration requirements. Laponite nanosilicates have been added to biomaterials to achieve biofunctional modification owing to their excellent biocompatibility and bioactivity. Herein, porous nanosilicate-functionalized polycaprolactone (PCL/LAP) was fabricated by 3D printing technology, and its bioactivities in bone regeneration were investigated in vitro and in vivo. In vitro experiments demonstrated that PCL/LAP exhibited good cytocompatibility and enhanced the viability of BMSCs. PCL/LAP functioned to stimulate osteogenic differentiation of BMSCs at the mRNA and protein levels and elevated angiogenic gene expression and cytokine secretion. Moreover, BMSCs cultured on PCL/LAP promoted the angiogenesis potential of endothelial cells by angiogenic cytokine secretion. Then, PCL/LAP scaffolds were implanted into the calvarial defect model. Toxicological safety of PCL/LAP was confirmed, and significant enhancement of vascularized bone formation was observed. Taken together, 3D-printed PCL/LAP scaffolds with brilliant osteogenesis to enhance bone regeneration could be envisaged as an outstanding bone substitute for a promising change in oral-maxillofacial bone defect reconstruction.

Delivering Proangiogenic Factors from 3D‐Printed Polycaprolactone Scaffolds for Vascularized Bone Regeneration

2020 ◽  
Vol 9 (23) ◽  
pp. 2000727
Author(s):  
Haoming Liu ◽  
Yingying Du ◽  
Gaojie Yang ◽  
Xixi Hu ◽  
Lin Wang ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
3D Printed ◽  
Vascularized Bone

scholarly journals Vascularization of Natural and Synthetic Bone Scaffolds

2018 ◽  
Vol 27 (8) ◽  
pp. 1269-1280 ◽  
Author(s):  
Xi Liu ◽  
Adam E. Jakus ◽  
Mehmet Kural ◽  
Hong Qian ◽  
Alexander Engler ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Bone Grafts ◽  
Specific Gene ◽  
Cell Seeding ◽  
Native Bone ◽  
Bone Scaffolds ◽  
3D Printed ◽  
Vascularized Bone ◽  
Natural And Synthetic

Vascularization of engineered bone tissue is critical for ensuring its survival after implantation. In vitro pre-vascularization of bone grafts with endothelial cells is a promising strategy to improve implant survival. In this study, we pre-cultured human smooth muscle cells (hSMCs) on bone scaffolds for 3 weeks followed by seeding of human umbilical vein endothelial cells (HUVECs), which produced a desirable environment for microvasculature formation. The sequential cell-seeding protocol was successfully applied to both natural (decellularized native bone, or DB) and synthetic (3D-printed Hyperelastic “Bone” scaffolds, or HB) scaffolds, demonstrating a comprehensive platform for developing natural and synthetic-based in vitro vascularized bone grafts. Using this sequential cell-seeding process, the HUVECs formed lumen structures throughout the DB scaffolds as well as vascular tissue bridging 3D-printed fibers within the HB. The pre-cultured hSMCs were essential for endothelial cell (EC) lumen formation within DB scaffolds, as well as for upregulating EC-specific gene expression of HUVECs grown on HB scaffolds. We further applied this co-culture protocol to DB scaffolds using a perfusion bioreactor, to overcome the limitations of diffusive mass transport into the interiors of the scaffolds. Compared with static culture, panoramic histological sections of DB scaffolds cultured in bioreactors showed improved cellular density, as well as a nominal increase in the number of lumen structures formed by ECs in the interior regions of the scaffolds. In conclusion, we have demonstrated that the sequential seeding of hSMCs and HUVECs can serve to generate early microvascular networks that could further support the in vitro tissue engineering of naturally or synthetically derived bone grafts and in both random (DB) and ordered (HB) pore networks. Combined with the preliminary bioreactor study, this process also shows potential to generate clinically sized, vascularized bone scaffolds for tissue and regenerative engineering.

Construction of a nanofiber network within 3D printed scaffolds for vascularized bone regeneration

2021 ◽  
Vol 9 (7) ◽  
pp. 2631-2646
Author(s):  
Mengru Geng ◽  
Qianqian Zhang ◽  
Jiani Gu ◽  
Jin Yang ◽  
Haibo Du ◽  
...  
Keyword(s):  
Cell Growth ◽  
Bone Formation ◽  
Bone Regeneration ◽  
Cell Growth And Migration ◽  
3D Printed ◽  
And Migration ◽  
Vascularized Bone

3D printed scaffolds with micro and nano architectures that facilitate cell growth and migration were prepared, and the scaffolds allowed deferoxamine release to accelerate bone formation.

Copper peptide-incorporated 3D-printed silk-based scaffolds promote vascularized bone regeneration

2021 ◽  
pp. 130147
Author(s):  
Mingliang Zhou ◽  
Xiaolin Wu ◽  
Jiaxin Luo ◽  
Guangzheng Yang ◽  
Yuezhi Lu ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
3D Printed ◽  
Vascularized Bone
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