Construction of a nanofiber network within 3D printed scaffolds for vascularized bone regeneration

2021 ◽  
Vol 9 (7) ◽  
pp. 2631-2646
Author(s):  
Mengru Geng ◽  
Qianqian Zhang ◽  
Jiani Gu ◽  
Jin Yang ◽  
Haibo Du ◽  
...  
Keyword(s):  
Cell Growth ◽  
Bone Formation ◽  
Bone Regeneration ◽  
Cell Growth And Migration ◽  
3D Printed ◽  
And Migration ◽  
Vascularized Bone

3D printed scaffolds with micro and nano architectures that facilitate cell growth and migration were prepared, and the scaffolds allowed deferoxamine release to accelerate bone formation.

Knockout of Ajuba Attenuates the Growth and Migration of Hepatocellular Carcinoma Cells

2020 ◽  
Vol 160 (11-12) ◽  
pp. 650-658
Author(s):  
Yichen Le ◽  
Yi He ◽  
Meirong Bai ◽  
Ying Wang ◽  
Jiaxue Wu ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Cell Growth ◽  
Cancer Progression ◽  
Normal Liver ◽  
Cell Growth And Migration ◽  
And Migration ◽  
Hcc Cells

Ajuba has been found to be mutated or aberrantly regulated in several human cancers and plays important roles in cancer progression via different signaling pathways. However, little is known about the role of Ajuba in hepatocellular carcinoma (HCC). Here, we found an upregulation of Ajuba expression in HCC tissues compared with normal liver tissues, while a poor prognosis was observed in HCC patients with high Ajuba expression. Knockout of Ajuba in HCC cells inhibited cell growth in vitro and in vivo, suppressed cell migration, and enhanced the cell apoptosis under stress. Moreover, re-expression of Ajuba in Ajuba-deficient cells could restore the phenotype of Ajuba-deficient cells. In conclusion, these results indicate that Ajuba is upregulated in HCC and promotes cell growth and migration of HCC cells, suggesting that Ajuba could possibly be a new target for HCC diagnosis and treatment.

scholarly journals Development of a Decellularized Porcine Esophageal Matrix for Potential Applications in Cancer Modeling

Cells ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 1055
Author(s):  
Hersh Chaitin ◽  
Michael L. Lu ◽  
Michael B. Wallace ◽  
Yunqing Kang
Keyword(s):  
Cell Growth ◽  
Lymphatic Vessels ◽  
Significant Loss ◽  
Cancer Cell Growth ◽  
Cell Growth And Migration ◽  
Decellularized Extracellular Matrix ◽  
Cancer Models ◽  
The Matrix ◽  
Potential Applications ◽  
And Migration

Many decellularized extracellular matrix-derived whole organs have been widely used in studies of tissue engineering and cancer models. However, decellularizing porcine esophagus to obtain decellularized esophageal matrix (DEM) for potential biomedical applications has not been widely investigated. In this study a modified decellularization protocol was employed to prepare a porcine esophageal DEM for the study of cancer cell growth. The cellular removal and retention of matrix components in the porcine DEM were fully characterized. The microstructure of the DEM was observed using scanning electronic microscopy. Human esophageal squamous cell carcinoma (ESCC) and human primary esophageal fibroblast cells (FBCs) were seeded in the DEM to observe their growth. Results show that the decellularization process did not cause significant loss of mechanical properties and that blood ducts and lymphatic vessels in the submucosa layer were also preserved. ESCC and FBCs grew on the DEM well and the matrix did not show any toxicity to cells. When FBS and ESCC were cocultured on the matrix, they secreted more periostin, a protein that supports cell adhesion on matrix. This study shows that the modified decellularization protocol can effectively remove the cell materials and maintain the microstructure of the porcine esophageal matrix, which has the potential application of studying cell growth and migration for esophageal cancer models.

scholarly journals Downregulation of miR-193a-3p inhibits cell growth and migration in renal cell carcinoma by targeting PTEN

Tumor Biology ◽  
2017 ◽  
Vol 39 (6) ◽  
pp. 101042831771195 ◽  
Author(s):  
Lingqi Liu ◽  
Yanqin Li ◽  
Shuchao Liu ◽  
Qixin Duan ◽  
Liang Chen ◽  
...  
Keyword(s):  
Renal Cell Carcinoma ◽  
Cell Growth ◽  
Cell Carcinoma ◽  
Renal Cell ◽  
Cell Growth And Migration ◽  
And Migration

scholarly journals 3D Printed Wesselsite Nanosheets Functionalized Scaffold Facilitates NIR‐II Photothermal Therapy and Vascularized Bone Regeneration

2021 ◽  
pp. 2100894
Author(s):  
Chen Yang ◽  
Hongshi Ma ◽  
Zhiyong Wang ◽  
Muhammad Rizwan Younis ◽  
Chunyang Liu ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Photothermal Therapy ◽  
3D Printed ◽  
Vascularized Bone

Ailanthone inhibits cell growth and migration of cisplatin resistant bladder cancer cells through down-regulation of Nrf2, YAP, and c-Myc expression.

Phytomedicine ◽  
2019 ◽  
Vol 56 ◽  
pp. 156-164 ◽  
Author(s):  
Martina Daga ◽  
Stefania Pizzimenti ◽  
Chiara Dianzani ◽  
Marie Angele Cucci ◽  
Roberta Cavalli ◽  
...  
Keyword(s):  
Bladder Cancer ◽  
Cell Growth ◽  
Cancer Cells ◽  
Down Regulation ◽  
Cell Growth And Migration ◽  
Bladder Cancer Cells ◽  
And Migration

scholarly journals Repair of critical-size porcine craniofacial bone defects using a collagen-polycaprolactone composite biomaterial

2021 ◽  
Author(s):  
Marley J Dewey ◽  
Derek J Milner ◽  
Daniel Weisgerber ◽  
Colleen Flanagan ◽  
Marcello Rubessa ◽  
...  
Keyword(s):  
Bone Formation ◽  
Bone Regeneration ◽  
Fibrous Tissue ◽  
Bone Defects ◽  
Large Animal ◽  
Collagen Scaffolds ◽  
Shape Fitting ◽  
Large Animal Models ◽  
3D Printed ◽  
Mineralized Collagen

Regenerative medicine approaches for massive craniomaxillofacial bone defects face challenges associated with the scale of missing bone, the need for rapid graft-defect integration, and challenges related to inflammation and infection. Mineralized collagen scaffolds have been shown to promote mesenchymal stem cell osteogenesis due to their porous nature and material properties, but are mechanically weak, limiting surgical practicality. Previously, these scaffolds were combined with 3D-printed polycaprolactone mesh to form a scaffold-mesh composite to increase strength and promote bone formation in sub-critical sized porcine ramus defects. Here, we compare the performance of mineralized collagen-polycaprolactone composites to the polycaprolactone mesh in a critical-sized porcine ramus defect model. While there were no differences in overall healing response between groups, our data demonstrated broadly variable metrics of healing regarding new bone infiltration and fibrous tissue formation. Abscesses were present surrounding some implants and polycaprolactone polymer was still present after 9-10 months of implantation. Overall, while there was limited successful healing, with 2 of 22 implants showed substantial levels of bone regeneration, and others demonstrating some form of new bone formation, the results suggest targeted improvements to improve repair of large animal models to more accurately represent craniomaxillofacial bone healing. Notably, strategies to increase osteogenesis throughout the implant, modulate the immune system to support repair, and employ shape-fitting tactics to avoid implant micromotion and resultant fibrosis. Improvements to the mineralized collagen scaffolds involve changes in pore size and shape to increase cell migration and osteogenesis and inclusion or delivery of factors to aid vascular ingrowth and bone regeneration.

scholarly journals cdc37 is essential for JNK pathway activation and wound closure in Drosophila

2019 ◽  
Vol 30 (21) ◽  
pp. 2651-2658
Author(s):  
Chan-wool Lee ◽  
Young-Chang Kwon ◽  
Youngbin Lee ◽  
Min-Yoon Park ◽  
Kwang-Min Choe
Keyword(s):  
Cell Growth ◽  
Wound Closure ◽  
Cell Growth And Migration ◽  
Jnk Pathway ◽  
Protein Levels ◽  
Pathway Activation ◽  
And Migration ◽  
The Stability ◽  
Jnk Activation

Wound closure in the Drosophila larval epidermis mainly involves nonproliferative, endocyling epithelial cells. Consequently, it is largely mediated by cell growth and migration. We discovered that both cell growth and migration in Drosophila require the cochaperone-encoding gene cdc37. Larvae lacking cdc37 in the epidermis failed to close wounds, and the cells of the epidermis failed to change cell shape and polarize. Likewise, wound-induced cell growth was significantly reduced, and correlated with a reduction in the size of the cell nucleus. The c-Jun N-terminal kinase (JNK) pathway, which is essential for wound closure, was not typically activated in injured cdc37 knockdown larvae. In addition, JNK, Hep, Mkk4, and Tak1 protein levels were reduced, consistent with previous reports showing that Cdc37 is important for the stability of various client kinases. Protein levels of the integrin β subunit and its wound-induced protein expression were also reduced, reflecting the disruption of JNK activation, which is crucial for expression of integrin β during wound closure. These results are consistent with a role of Cdc37 in maintaining the stability of the JNK pathway kinases, thus mediating cell growth and migration during Drosophila wound healing.

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