Schwann Cell Contact Guidance versus Boundary ­Interaction in Functional Wound Healing along Nano and Microstructured Membranes

2015 ◽  
Vol 4 (12) ◽  
pp. 1849-1860 ◽  
Author(s):  
Ilaria Tonazzini ◽  
Emanuela Jacchetti ◽  
Sandro Meucci ◽  
Fabio Beltram ◽  
Marco Cecchini
Keyword(s):  
Wound Healing ◽  
Schwann Cell ◽  
Contact Guidance ◽  
Cell Contact ◽  
Boundary Interaction

scholarly journals Screening Platform for Cell Contact Guidance Based on Inorganic Biomaterial Micro/nanotopographical Gradients

2017 ◽  
Vol 9 (37) ◽  
pp. 31433-31445 ◽  
Author(s):  
Qihui Zhou ◽  
Olga Castañeda Ocampo ◽  
Carlos F. Guimarães ◽  
Philipp T. Kühn ◽  
Theo G. van Kooten ◽  
...  
Keyword(s):  
Contact Guidance ◽  
Cell Contact ◽  
Screening Platform

Easy fabrication of aligned PLLA nanofibers-based 2D scaffolds suitable for cell contact guidance studies

2016 ◽  
Vol 62 ◽  
pp. 301-306 ◽  
Author(s):  
John Mohanraj ◽  
Luca Puzzi ◽  
Ennio Capria ◽  
Stefania Corvaglia ◽  
Loredana Casalis ◽  
...  
Keyword(s):  
Contact Guidance ◽  
Cell Contact

An Anisotropic Biphasic Theory of Tissue-Equivalent Mechanics: The Interplay Among Cell Traction, Fibrillar Network Deformation, Fibril Alignment, and Cell Contact Guidance

1997 ◽  
Vol 119 (2) ◽  
pp. 137-145 ◽  
Author(s):  
V. H. Barocas ◽  
R. T. Tranquillo
Keyword(s):  
Contact Guidance ◽  
Deformation Tensor ◽  
Cell Contact ◽  
Cell Alignment ◽  
Collagen Gels ◽  
Cell Orientation ◽  
Collagen Network ◽  
Orientation Tensor ◽  
Tissue Equivalents ◽  
Cell Traction

We present a general mathematical theory for the mechanical interplay in tissue-equivalents (cell-populated collagen gels): Cell traction leads to compaction of the fibrillar collagen network, which for certain conditions such as a mechanical constraint or inhomogeneous cell distribution, can result in inhomogeneous compaction and consequently fibril alignment, leading to cell contact guidance, which affects the subsequent compaction. The theory accounts for the intrinsically biphasic nature of collagen gel, which is comprised of collagen network and interstitial solution. The theory also accounts for fibril alignment due to inhomogeneous network deformation, that is, anisotropic strain, and for cell alignment in response to fibril alignment. Cell alignment results in anisotropic migration and traction, as modeled by a cell orientation tensor that is a function of a fiber orientation tensor, which is defined by the network deformation tensor. Models for a variety of tissue-equivalents are shown to predict qualitatively the alignment that arises due to inhomogeneous compaction driven by cell traction.

scholarly journals Transfer of assembled collagen fibrils to flexible substrates for mechanically tunable contact guidance cues

2018 ◽  
Vol 10 (11) ◽  
pp. 705-718 ◽  
Author(s):  
Juan Wang ◽  
Joseph Koelbl ◽  
Anuraag Boddupalli ◽  
Zhiqi Yao ◽  
Kaitlin M. Bratlie ◽  
...  
Keyword(s):  
Wound Healing ◽  
Cancer Invasion ◽  
Collagen Fibrils ◽  
Contact Guidance ◽  
Flexible Substrates ◽  
Guidance Cues ◽  
Aligned Fibers

Contact guidance or bidirectional migration along aligned fibers modulates many physiological and pathological processes such as wound healing and cancer invasion.

Directing Osteoblast Alignment and Elongation on the Micro-Grooved Silica-Based Hybrid Membrane

2013 ◽  
Vol 647 ◽  
pp. 165-169 ◽  
Author(s):  
Song Chen ◽  
Nobutaka Hanagata
Keyword(s):  
Bone Tissue ◽  
Potential Application ◽  
Hybrid Membrane ◽  
Contact Guidance ◽  
Cell Contact ◽  
Hybrid Membranes ◽  
Pdms Mold

Silica-chitosan hybrid was synthesized from a mixture of chitosan and glycidyloxypropyltrimethoxysilane and then coated on a microgrooved polydimethylsiloxane (PDMS) mold to produce the microgrooved silica-chitosan hybrid membrane. After incubated with osteoblast-like MC3T3-E1 cells, the microgrooved hybrid membranes strongly directed the alignment and elongation of cells and exhibited a strong cell contact guidance ability, indicating a potential application as engineering bone tissue regenerative materials.

scholarly journals Regulation of Endothelial Cell Motility by Complexes of Tetraspan Molecules CD81/TAPA-1 and CD151/PETA-3 with α3β1 Integrin Localized at Endothelial Lateral Junctions

1998 ◽  
Vol 141 (3) ◽  
pp. 791-804 ◽  
Author(s):  
María Yáñez-Mó ◽  
Arántzazu Alfranca ◽  
Carlos Cabañas ◽  
Mónica Marazuela ◽  
Reyes Tejedor ◽  
...  
Keyword(s):  
Wound Healing ◽  
Endothelial Cell ◽  
Cell Motility ◽  
Cell Polarization ◽  
Cell Junctions ◽  
Cell Junction ◽  
Cell Contact ◽  
Collagen Gels ◽  
Α3β1 Integrin ◽  
Cell Cell

Cell-to-cell junction structures play a key role in cell growth rate control and cell polarization. In endothelial cells (EC), these structures are also involved in regulation of vascular permeability and leukocyte extravasation. To identify novel components in EC intercellular junctions, mAbs against these cells were produced and selected using a morphological screening by immunofluorescence microscopy. Two novel mAbs, LIA1/1 and VJ1/16, specifically recognized a 25-kD protein that was selectively localized at cell–cell junctions of EC, both in the primary formation of cell monolayers and when EC reorganized in the process of wound healing. This antigen corresponded to the recently cloned platelet-endothelial tetraspan antigen CD151/PETA-3 (platelet-endothelial tetraspan antigen-3), and was consistently detected at EC cell–cell contact sites. In addition to CD151/PETA-3, two other members of the tetraspan superfamily, CD9 and CD81/ TAPA-1 (target of antiproliferative antibody-1), localized at endothelial cell-to-cell junctions. Biochemical analysis demonstrated molecular associations among tetraspan molecules themselves and those of CD151/ PETA-3 and CD9 with α3β1 integrin. Interestingly, mAbs directed to both CD151/PETA-3 and CD81/ TAPA-1 as well as mAb specific for α3 integrin, were able to inhibit the migration of ECs in the process of wound healing. The engagement of CD151/PETA-3 and CD81/TAPA-1 inhibited the movement of individual ECs, as determined by quantitative time-lapse video microscopy studies. Furthermore, mAbs against the CD151/PETA-3 molecule diminished the rate of EC invasion into collagen gels. In addition, these mAbs were able to increase the adhesion of EC to extracellular matrix proteins. Together these results indicate that CD81/TAPA-1 and CD151/PETA-3 tetraspan molecules are components of the endothelial lateral junctions implicated in the regulation of cell motility, either directly or by modulation of the function of the associated integrin heterodimers.

Response of single, pairs, and clusters of epithelial cells to substratum topography

1995 ◽  
Vol 73 (7-8) ◽  
pp. 473-489 ◽  
Author(s):  
C. Oakley ◽  
D. M. Brunette
Keyword(s):  
Epithelial Cells ◽  
Actin Filaments ◽  
Cell Shape ◽  
Single Cells ◽  
Cell Spreading ◽  
Contact Guidance ◽  
Adjacent Cell ◽  
Cell Contact ◽  
Topographic Effects ◽  
Control Surfaces

Cells cultured on grooved substrata change their shape, orientation, and direction of locomotion in response to substratum topography, a phenomenon called contact or topographic guidance. Porcine epithelial cells (E-cells) spread on micromachined grooved or smooth control surfaces were examined by epifluorescence and confocal microscopy to determine area, cell shape, and orientation in conjunction with distributions and orientations of actin filaments and microtubules. Single cells, cells within a pair or cluster, and pairs or clusters considered as a unit were compared. As expected, cell contact increased cell spreading, but surprisingly, increased cell contact influenced cell shape on smooth and grooved surfaces and increased alignment of cells spread on grooves. Both actin filaments and microtubules aligned initially and most consistently along the walls and ridge–groove edges. Single E-cells displayed the least variability of aligned cytoskeletal patterns. E-cells within clusters displayed the most variability as local topographic effects on the cytoskeleton could be overridden by adjacent cell contact. Overall, contact guidance of E-cells was neither synonymous with nor contingent upon an elliptical morphology oriented to the topography. E-cells also differed from fibroblasts in their response to cell contact and in their lack of a relationship between cell polarity and locomotion.Key words: microtubules, actin, topographic guidance, micromachined substrata.

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