scholarly journals Transfer of assembled collagen fibrils to flexible substrates for mechanically tunable contact guidance cues

2018 ◽  
Vol 10 (11) ◽  
pp. 705-718 ◽  
Author(s):  
Juan Wang ◽  
Joseph Koelbl ◽  
Anuraag Boddupalli ◽  
Zhiqi Yao ◽  
Kaitlin M. Bratlie ◽  
...  
Keyword(s):  
Wound Healing ◽  
Cancer Invasion ◽  
Collagen Fibrils ◽  
Contact Guidance ◽  
Flexible Substrates ◽  
Guidance Cues ◽  
Aligned Fibers

Contact guidance or bidirectional migration along aligned fibers modulates many physiological and pathological processes such as wound healing and cancer invasion.

scholarly journals Collagen Fibrils Mechanically Contribute to Tissue Contraction in an In Vitro Wound Healing Scenario

2019 ◽  
Vol 6 (9) ◽  
pp. 1801780 ◽  
Author(s):  
Erik Brauer ◽  
Evi Lippens ◽  
Oliver Klein ◽  
Grit Nebrich ◽  
Sophie Schreivogel ◽  
...  
Keyword(s):  
Wound Healing ◽  
Collagen Fibrils ◽  
Tissue Contraction

scholarly journals Epitaxially Grown Collagen Fibrils Reveal Diversity in Contact Guidance Behavior among Cancer Cells

Langmuir ◽  
2014 ◽  
Vol 31 (1) ◽  
pp. 307-314 ◽  
Author(s):  
Juan Wang ◽  
Joseph W. Petefish ◽  
Andrew C. Hillier ◽  
Ian C. Schneider
Keyword(s):  
Cancer Cells ◽  
Collagen Fibrils ◽  
Contact Guidance

Hepatocyte growth factor inhibits the formation of the basement membrane of alveolar epithelial cells in vitro

2004 ◽  
Vol 286 (5) ◽  
pp. L939-L946 ◽  
Author(s):  
Akiko Furuyama ◽  
Katsumi Mochitate
Keyword(s):  
Wound Healing ◽  
Growth Factor ◽  
Epithelial Cells ◽  
Basement Membrane ◽  
Matrix Metalloproteinase ◽  
Hepatocyte Growth Factor ◽  
Collagen Fibrils ◽  
Membrane Formation ◽  
Hepatocyte Growth

Hepatocyte growth factor (HGF) is a pulmotrophic factor for the regeneration of injured pulmonary tissue. We investigated the role of HGF in basement membrane formation during wound healing by immortalized alveolar type II epithelial cells that could form a continuous basement membrane when they were cultured on collagen fibrils in the presence of entactin-contaminated laminin-1. Cells cultured with 5.0 ng/ml HGF neither formed a continuous basement membrane on collagen fibrils nor maintained a continuous basement membrane architecture on a basement membrane substratum. The cells showed increased secretion of matrix metalloproteinase-9 and urokinase-type plasminogen activator, and the HGF-induced inhibition of basement membrane formation was attenuated by addition of 200 ng/ml tissue inhibitor of matrix metalloproteinase-1. Cells sequentially exposed to HGF and 1.0 ng/ml transforming growth factor-β1 had enhanced basement membrane formation compared with those receiving these reagents in the reverse order or concurrently. HGF simultaneously stimulated proliferation and migration of the cells so that it advanced wound closure on the basement membrane substratum. The present results indicate that the role of HGF in wound healing is the stimulation of reepithelization, but this factor may also contribute to the degradation of the basement membrane.

scholarly journals Anomalous ipsilateral optic fibre projection in Xenopus induced by larval tectal ablation

Development ◽  
1979 ◽  
Vol 50 (1) ◽  
pp. 111-122
Author(s):  
Charles Straznicky ◽  
John Glastonbury
Keyword(s):  
Contact Guidance ◽  
Retinal Projection ◽  
Guidance Cues ◽  
Optic Fibre ◽  
The Right

Unilateral left tectal ablation was carried out in Xenopus between stage 48 and 1 month after metamorphosis. Six to 12 weeks after metamorphosis the retinal projection from the right eye was examined with the use of [3H]proline autoradiography. The autoradiographs indicated that optic fibres whose tectal target was destroyed recrossed to the ipsilateral tectum and basal optic nucleus via the posterior and pretectal commissures. No anomalous recrossing occurred if the tectal ablation was carried out at stage 58 or later. The aberrant optic fibres were restricted to the rostrolateral and central parts of the ipsilateral tectum and they terminated in a discontinuous manner. It is concluded that available surfaces serving as contact guidance cues are needed to direct aberrant optic fibres to the ipsilateral tectum.

Electron Microscopy of Wound Healing in Patients with Hernia

1975 ◽  
Vol 33 ◽  
pp. 352-353
Author(s):  
C.N. Sun ◽  
H.J. White ◽  
R.C. Read
Keyword(s):  
Electron Microscopy ◽  
Wound Healing ◽  
Phosphate Buffer ◽  
Osmium Tetroxide ◽  
Phosphotungstic Acid ◽  
Rectus Sheath ◽  
Uranyl Acetate ◽  
Collagen Fibrils ◽  
Lead Citrate ◽  
Native Collagen

Previously we have reported the defect of collagen fibrils from herniated rectus sheath. This presentation includes additional sections from postsurgical incisions (10 days) from both control and hernia patients. Small pieces of rectus sheath were fixed in 3% glutaraldehyde in phosphate buffer (pH 7.2) and post fixed with buffered 2% osmium tetroxide. The tissues were then dehydrated in serially increasing concentrations of alcohol and embedded in Epon 812. Sections were stained with 2.5% phosphotungstic acid or uranyl acetate and lead citrate.Previously we found that collagen fibrils from "non-herniated" rectus sheath have uniform diameters and 640 Å periodicity with seven or more intraperiodic bands resembling typical native collagen fibrils, while the fibrils from fascia obtained from patients with direct herniation show considerable variation in diameter. These variations are often found in the same individual fibers with a range from 300 Å to 3000 Å.

A Finite Element Solution for the Anisotropic Biphasic Theory of Tissue-Equivalent Mechanics: The Effect of Contact Guidance on Isometric Cell Traction Measurement

1997 ◽  
Vol 119 (3) ◽  
pp. 261-268 ◽  
Author(s):  
V. H. Barocas ◽  
R. T. Tranquillo
Keyword(s):  
Finite Element Method ◽  
Finite Element ◽  
Mixed Finite Element ◽  
Collagen Fibrils ◽  
Contact Guidance ◽  
Sample Length ◽  
Tissue Equivalent ◽  
Cell Traction ◽  
Anisotropic Biphasic Theory ◽  
Element Method

We present a method for solving the governing equations from our anisotropic biphasic theory of tissue-equivalent mechanics (Barocas and Tranquillo, 1997) for axisymmetric problems. A mixed finite element method is used for discretization of the spatial derivatives, and the DASPK subroutine (Brown et al., 1994) is used to solve the resulting differential-algebraic equation system. The preconditioned GMRES algorithm, using a preconditioner based on an extension of Dembo’s (1994) adaptation of the Uzawa algorithm for viscous flows, provides an efficient and scaleable solution method, with the finite element method discretization being first-order accurate in space. In the cylindrical isometric cell traction assay, the chosen test problem, a cylindrical tissue equivalent is adherent at either end to fixed circular platens. As the cells exert traction on the collagen fibrils, the force required to maintain constant sample length, or load, is measured. However, radial compaction occurs during the course of the assay, so that the cell and network concentrations increase and collagen fibrils become aligned along the axis of the cylinder, leading to cell alignment along the axis. Our simulations predict that cell contact guidance leads to an increase in the load measured in the assay, but this effect is diminished by the tendency of contact guidance to inhibit radial compaction of the sample, which in turn reduces concentrations and hence the measured load.

scholarly journals Collective cell migration: Implications for wound healing and cancer invasion

2013 ◽  
Vol 1 (1) ◽  
pp. 21 ◽  
Author(s):  
Jianxin Jiang ◽  
Li Li ◽  
Yong He ◽  
Min Zhao
Keyword(s):  
Wound Healing ◽  
Cell Migration ◽  
Cancer Invasion ◽  
Collective Cell Migration

scholarly journals Mesoscale substrate curvature overrules nanoscale contact guidance to direct bone marrow stromal cell migration

2018 ◽  
Vol 15 (145) ◽  
pp. 20180162 ◽  
Author(s):  
Maike Werner ◽  
Nicholas A. Kurniawan ◽  
Gabriela Korus ◽  
Carlijn V. C. Bouten ◽  
Ansgar Petersen
Keyword(s):  
Bone Marrow ◽  
Cellular Response ◽  
Large Scale ◽  
Biological Tissues ◽  
Collagen Fibrils ◽  
Contact Guidance ◽  
Cylinder Axis ◽  
Cylinder Diameter ◽  
Substrate Curvature ◽  
And Migration

The intrinsic architecture of biological tissues and of implanted biomaterials provides cells with large-scale geometrical cues. To understand how cells are able to sense and respond to complex structural environments, a deeper insight into the cellular response to multi-scale and conflicting geometrical cues is needed. In this study, we subjected human bone marrow stromal cells (hBMSCs) to mesoscale cylindrical surfaces (diameter 250–5000 µm) and nanoscale collagen fibrils (diameter 100–200 nm) that were aligned perpendicular to the cylinder axis. On flat surfaces and at low substrate curvatures (cylinder diameter d > 1000 µm), cell alignment and migration were governed by the nanoscale collagen fibrils, consistent with the contact guidance effect. With increasing surface curvature (decreasing cylinder diameter, d < 1000 µm), cells increasingly aligned and migrated along the cylinder axis, i.e. the direction of zero curvature. An increase in phosphorylated myosin light chain levels was observed with increasing substrate curvature, suggesting a link between substrate-induced cell bending and the F-actin–myosin machinery. Taken together, this work demonstrates that geometrical cues of up to 10× cell size can play a dominant role in directing hBMSC alignment and migration and that the effect of nanoscale contact guidance can even be overruled by mesoscale curvature guidance.

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