scholarly journals Early Cancer Diagnosis: Platelet-Leukocyte Hybrid Membrane-Coated Immunomagnetic Beads for Highly Efficient and Highly Specific Isolation of Circulating Tumor Cells (Adv. Funct. Mater. 34/2018)

2018 ◽  
Vol 28 (34) ◽  
pp. 1870241
Author(s):  
Lang Rao ◽  
Qian-Fang Meng ◽  
Qinqin Huang ◽  
Zixiang Wang ◽  
Guang-Tao Yu ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Cancer Diagnosis ◽  
Early Cancer ◽  
Hybrid Membrane ◽  
Immunomagnetic Beads ◽  
Highly Efficient ◽  
Early Cancer Diagnosis

Nanotopographic Biomaterials for Isolation of Circulating Tumor Cells

2014 ◽  
Vol 5 (4) ◽  
Author(s):  
Weiyi Qian ◽  
Yan Zhang ◽  
Andrew Gordon ◽  
Weiqiang Chen
Keyword(s):  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Cancer Diagnosis ◽  
Cancer Metastasis ◽  
Early Cancer ◽  
Tumor Mass ◽  
Early Cancer Diagnosis ◽  
Capture And Release ◽  
Increasing Demand ◽  
Detection Technologies

Circulating tumor cells (CTCs) shed from the primary tumor mass and circulating in the bloodstream of patients are believed to be vital to understand of cancer metastasis and progression. Capture and release of CTCs for further enumeration and molecular characterization holds the key for early cancer diagnosis, prognosis and therapy evaluation. However, detection of CTCs is challenging due to their rarity, heterogeneity and the increasing demand of viable CTCs for downstream biological analysis. Nanotopographic biomaterial-based microfluidic systems are emerging as promising tools for CTC capture with improved capture efficiency, purity, throughput and retrieval of viable CTCs. This review offers a brief overview of the recent advances in this field, including CTC detection technologies based on nanotopographic biomaterials and relevant nanofabrication methods. Additionally, the possible intracellular mechanisms of the intrinsic nanotopography sensitive responses that lead to the enhanced CTC capture are explored.

scholarly journals Current detection technologies for circulating tumor cells

2017 ◽  
Vol 46 (8) ◽  
pp. 2038-2056 ◽  
Author(s):  
Zheyu Shen ◽  
Aiguo Wu ◽  
Xiaoyuan Chen
Keyword(s):  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Cancer Diagnosis ◽  
Early Cancer ◽  
Cancer Recurrence ◽  
Early Cancer Diagnosis ◽  
Chemotherapeutic Efficacy ◽  
Anti Cancer ◽  
Current Detection ◽  
Detection Technologies

CTC detection can be used for early cancer diagnosis, earlier evaluation of cancer recurrence and chemotherapeutic efficacy, and choice of individual sensitive anti-cancer drugs.

Engineered red blood cells for capturing circulating tumor cells with high performance

Nanoscale ◽  
2018 ◽  
Vol 10 (13) ◽  
pp. 6014-6023 ◽  
Author(s):  
Dao-Ming Zhu ◽  
Lei Wu ◽  
Meng Suo ◽  
Song Gao ◽  
Wei Xie ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Cancer Diagnosis ◽  
Peripheral Blood ◽  
Blood Cells ◽  
High Performance ◽  
Early Cancer ◽  
Treatment Monitoring ◽  
Prognostic Evaluation ◽  
Early Cancer Diagnosis

Filtration of circulating tumor cells (CTCs) in peripheral blood is of proven importance for early cancer diagnosis, treatment monitoring, metastasis diagnosis, and prognostic evaluation.

Platelet-Leukocyte Hybrid Membrane-Coated Immunomagnetic Beads for Highly Efficient and Highly Specific Isolation of Circulating Tumor Cells

2018 ◽  
Vol 28 (34) ◽  
pp. 1803531 ◽  
Author(s):  
Lang Rao ◽  
Qian-Fang Meng ◽  
Qinqin Huang ◽  
Zixiang Wang ◽  
Guang-Tao Yu ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Hybrid Membrane ◽  
Immunomagnetic Beads ◽  
Highly Efficient

scholarly journals Nanotechnology-Based Strategies for Early Cancer Diagnosis Using Circulating Tumor Cells as a Liquid Biopsy

2018 ◽  
Vol 2 (1) ◽  
pp. 21-41 ◽  
Author(s):  
Qinqin Huang ◽  
Yin Wang ◽  
Xingxiang Chen ◽  
Yimeng Wang ◽  
Zhiqiang Li ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Cancer Diagnosis ◽  
Liquid Biopsy ◽  
Early Cancer ◽  
Early Cancer Diagnosis

High Sensitivity Detection of Tumor Cells in Biological Samples using Multivalent Aptamer Strand Displacement Strategy

The Analyst ◽  
2022 ◽  
Author(s):  
Jieru Xu ◽  
Jiahui Xiang ◽  
Jialing Chen ◽  
Tao Wan ◽  
Hongli Deng ◽  
...  
Keyword(s):  
Cell Surface ◽  
Tumor Cells ◽  
Cancer Diagnosis ◽  
Biological Samples ◽  
Cell Membranes ◽  
Early Cancer ◽  
High Sensitivity ◽  
Strand Displacement ◽  
Early Cancer Diagnosis ◽  
High Sensitivity Detection

Monitoring the cell surface-expressed nucleolin facilitates early cancer diagnosis. Herein, we developed multivalent aptamer displacement strand duplex strategy on the cell membranes utilizing a multi-receptor co-recognition design for improving sensitivity...

scholarly journals Circulating Tumor Cells from Enumeration to Analysis: Current Challenges and Future Opportunities

Cancers ◽  
2021 ◽  
Vol 13 (11) ◽  
pp. 2723
Author(s):  
Yu-Ping Yang ◽  
Teresa M. Giret ◽  
Richard J. Cote
Keyword(s):  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Ex Vivo ◽  
Detection Sensitivity ◽  
Clinical Utilization ◽  
Diagnosis And Prognosis ◽  
Early Cancer Diagnosis ◽  
Potential Marker ◽  
Potential Applications ◽  
Downstream Analysis

Circulating tumor cells (CTCs) have been recognized as a major contributor to distant metastasis. Their unique role as metastatic seeds renders them a potential marker in the circulation for early cancer diagnosis and prognosis as well as monitoring of therapeutic response. In the past decade, researchers mainly focused on the development of isolation techniques for improving the recovery rate and purity of CTCs. These developed techniques have significantly increased the detection sensitivity and enumeration accuracy of CTCs. Currently, significant efforts have been made toward comprehensive molecular characterization, ex vivo expansion of CTCs, and understanding the interactions between CTCs and their associated cells (e.g., immune cells and stromal cells) in the circulation. In this review, we briefly summarize existing CTC isolation technologies and specifically focus on advances in downstream analysis of CTCs and their potential applications in precision medicine. We also discuss the current challenges and future opportunities in their clinical utilization.

scholarly journals A Modified Method to Isolate Circulating Tumor Cells and Identify by a Panel of Gene Mutations in Lung Cancer

2021 ◽  
Vol 20 ◽  
pp. 153303382199527
Author(s):  
Helin Wang ◽  
Jieqing Wu ◽  
Qi Zhang ◽  
Jianqing Hao ◽  
Ying Wang ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Detection Rate ◽  
Target Genes ◽  
White Blood Cells ◽  
Gene Mutations ◽  
Copy Number Variations ◽  
Immunomagnetic Beads ◽  
Membrane Rupture

The CellSearch system is the only FDA approved and successful used detection technology for circulating tumor cells(CTCs). However, the process for identification of CTCs by CellSearch appear to damage the cells, which may adversely affects subsequent molecular biology assays. We aimed to explore and establish a membrane-preserving method for immunofluorescence identification of CTCs that keeping the isolated cells intact. 98 patients with lung cancer were enrolled, and the efficacy of clinical detection of CTCs was examined. Based on the CellSearch principle, we optimized an anti-EpCAM antibody and improved cell membrane rupture. A 5 ml peripheral blood sample was used to enrich CTCs with EpCAM immunomagnetic beads. Fluorescence signals were amplified with secondary antibodies against anti-EpCAM antibody attached on immunomagnetic beads. After identifying CTCs, single CTCs were isolated by micromanipulation. To confirm CTCs, genomic DNA was extracted and amplified at the single cell level to sequence 72 target genes of lung cancer and analyze the mutation copy number variations (CNVs) and gene mutations. A goat anti-mouse polyclonal antibody conjugated with Dylight 488 was selected to stain tumor cells. We identified CTCs based on EpCAM+ and CD45+ cells to exclude white blood cells. In the 98 lung cancer patients, the detection rate of CTCs (≥1 CTC) per 5 ml blood was 87.76%, the number of detections was 1–36, and the median was 2. By sequencing 72 lung cancer-associated genes, we found a high level of CNVs and gene mutations characteristic of tumor cells. We established a new CTCs staining scheme that significantly improves the detection rate and allows further analysis of CTCs characteristics at the genetic level.

scholarly journals Aptamer-Conjugated Graphene Oxide Membranes for Highly Efficient Capture and Accurate Identification of Multiple Types of Circulating Tumor Cells

2015 ◽  
Vol 26 (2) ◽  
pp. 235-242 ◽  
Author(s):  
Bhanu Priya Viraka Nellore ◽  
Rajashekhar Kanchanapally ◽  
Avijit Pramanik ◽  
Sudarson Sekhar Sinha ◽  
Suhash Reddy Chavva ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Accurate Identification ◽  
Highly Efficient ◽  
Oxide Membranes ◽  
Graphene Oxide Membranes
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