Platelet-Leukocyte Hybrid Membrane-Coated Immunomagnetic Beads for Highly Efficient and Highly Specific Isolation of Circulating Tumor Cells

2018 ◽  
Vol 28 (34) ◽  
pp. 1803531 ◽  
Author(s):  
Lang Rao ◽  
Qian-Fang Meng ◽  
Qinqin Huang ◽  
Zixiang Wang ◽  
Guang-Tao Yu ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Hybrid Membrane ◽  
Immunomagnetic Beads ◽  
Highly Efficient

scholarly journals A Modified Method to Isolate Circulating Tumor Cells and Identify by a Panel of Gene Mutations in Lung Cancer

2021 ◽  
Vol 20 ◽  
pp. 153303382199527
Author(s):  
Helin Wang ◽  
Jieqing Wu ◽  
Qi Zhang ◽  
Jianqing Hao ◽  
Ying Wang ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Detection Rate ◽  
Target Genes ◽  
White Blood Cells ◽  
Gene Mutations ◽  
Copy Number Variations ◽  
Immunomagnetic Beads ◽  
Membrane Rupture

The CellSearch system is the only FDA approved and successful used detection technology for circulating tumor cells(CTCs). However, the process for identification of CTCs by CellSearch appear to damage the cells, which may adversely affects subsequent molecular biology assays. We aimed to explore and establish a membrane-preserving method for immunofluorescence identification of CTCs that keeping the isolated cells intact. 98 patients with lung cancer were enrolled, and the efficacy of clinical detection of CTCs was examined. Based on the CellSearch principle, we optimized an anti-EpCAM antibody and improved cell membrane rupture. A 5 ml peripheral blood sample was used to enrich CTCs with EpCAM immunomagnetic beads. Fluorescence signals were amplified with secondary antibodies against anti-EpCAM antibody attached on immunomagnetic beads. After identifying CTCs, single CTCs were isolated by micromanipulation. To confirm CTCs, genomic DNA was extracted and amplified at the single cell level to sequence 72 target genes of lung cancer and analyze the mutation copy number variations (CNVs) and gene mutations. A goat anti-mouse polyclonal antibody conjugated with Dylight 488 was selected to stain tumor cells. We identified CTCs based on EpCAM+ and CD45+ cells to exclude white blood cells. In the 98 lung cancer patients, the detection rate of CTCs (≥1 CTC) per 5 ml blood was 87.76%, the number of detections was 1–36, and the median was 2. By sequencing 72 lung cancer-associated genes, we found a high level of CNVs and gene mutations characteristic of tumor cells. We established a new CTCs staining scheme that significantly improves the detection rate and allows further analysis of CTCs characteristics at the genetic level.

scholarly journals Aptamer-Conjugated Graphene Oxide Membranes for Highly Efficient Capture and Accurate Identification of Multiple Types of Circulating Tumor Cells

2015 ◽  
Vol 26 (2) ◽  
pp. 235-242 ◽  
Author(s):  
Bhanu Priya Viraka Nellore ◽  
Rajashekhar Kanchanapally ◽  
Avijit Pramanik ◽  
Sudarson Sekhar Sinha ◽  
Suhash Reddy Chavva ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Accurate Identification ◽  
Highly Efficient ◽  
Oxide Membranes ◽  
Graphene Oxide Membranes

Integration of aligned polymer nanofibers within a microfluidic chip for efficient capture and rapid release of circulating tumor cells

2018 ◽  
Vol 2 (5) ◽  
pp. 891-900 ◽  
Author(s):  
Yunchao Xiao ◽  
Mengyuan Wang ◽  
Lizhou Lin ◽  
Lianfang Du ◽  
Mingwu Shen ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Microfluidic Chip ◽  
Polymer Nanofibers ◽  
Highly Efficient ◽  
Rapid Release ◽  
Aligned Nanofibers

Zwitterion-functionalized aligned nanofibers integrated with a microfluidic chip can be used for highly efficient capture and rapid release of CTCs.

scholarly journals Highly efficient capture of circulating tumor cells by microarray in a microfluidic device

2019 ◽  
Vol 33 (S1) ◽  
Author(s):  
Hui‐Yu Liu ◽  
Claudia Hille ◽  
Anna Haller ◽  
Ravi Kumar ◽  
Klaus Pantel ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Microfluidic Device ◽  
Highly Efficient

Highly efficient capture of circulating tumor cells with low background signals by using pyramidal microcavity array

2019 ◽  
Vol 1060 ◽  
pp. 133-141 ◽  
Author(s):  
Jiaxiang Yin ◽  
Lei Mou ◽  
Mingzhu Yang ◽  
Wenwu Zou ◽  
Chang Du ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Low Background ◽  
Highly Efficient
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