A multi-coordinating polymer ligand optimized for the functionalization of metallic nanocrystals and nanorods

2016 ◽  
Vol 191 ◽  
pp. 481-494 ◽  
Author(s):  
Wentao Wang ◽  
Xin Ji ◽  
Hunter Burns ◽  
Hedi Mattoussi
Keyword(s):  
Gold Nanoparticles ◽  
Colloidal Stability ◽  
Protein Corona ◽  
Growth Media ◽  
Nucleophilic Addition Reaction ◽  
One Step ◽  
Nonspecific Interactions ◽  
Anchoring Groups ◽  
Polymer Ligand

We report the design and use of a multi-coordinating polymer ligand that is ideally suited for functionalizing gold nanoparticles and nanorods, and promoting their steric stabilization in buffer media. The ligand is prepared via a one-step nucleophilic addition reaction between poly(isobutylene-alt-maleic anhydride) and amine-modified anchoring groups and hydrophilic moieties. Surface functionalization of gold nanoparticles and nanorods with this polymer yields nanocrystals that exhibit excellent long-term colloidal stability over a broad range of conditions, including pH changes and in growth media, as verified using dynamic light scattering measurements combined with agarose gel electrophoresis. This polymer coating can also prevent the formation of protein corona. These features bode well for use in biological applications where small size, reduced nonspecific interactions and colloidal stability are highly desired. Furthermore, this design can be easily expanded to functionalize a variety of other inorganic nanocrystals.

Surface-Functionalizing Metal, Metal Oxide and Semiconductor Nanocrystals with a Multi-coordinating Polymer Platform

MRS Advances ◽  
2016 ◽  
Vol 1 (56) ◽  
pp. 3741-3747 ◽  
Author(s):  
Wentao Wang ◽  
Xin Ji ◽  
Anshika Kapur ◽  
Hedi Mattoussi
Keyword(s):  
Self Assembly ◽  
Addition Reaction ◽  
Semiconductor Nanocrystals ◽  
Long Term Stability ◽  
Sensing Platforms ◽  
Metal Metal ◽  
Nucleophilic Addition Reaction ◽  
One Step ◽  
Anchoring Groups

ABSTRACTWe introduce a new set of multifunctional metal-coordinating polymers as ligands for the surface functionalization of three different inorganic nanocrystals: luminescent quantum dots (QDs), magnetic iron oxide nanocrystals and metal gold nanoparticles. The ligand design relies on the introduction of a large but controllable number of anchoring groups, hydrophilic moieties and reactive functionalities all in the same polymer chain, via a one-step nucleophilic addition reaction. Nanocrystals capped with these polymer ligands exhibit long-term stability over a broad range of biological conditions. Furthermore, when zwitterion groups are introduced as hydrophilic blocks, this yields a compact ligand coating that allows conjugation of biomolecules to the nanocrystals via metal-histidine self-assembly. The resulting hydrophilic nanocrystals have been used to develop a few specific sensing platforms targeting soluble iron ions and cysteine.

A multifunctional amphiphilic polymer as a platform for surface-functionalizing metallic and other inorganic nanostructures

2014 ◽  
Vol 175 ◽  
pp. 137-151 ◽  
Author(s):  
Wentao Wang ◽  
Fadi Aldeek ◽  
Xin Ji ◽  
Birong Zeng ◽  
Hedi Mattoussi
Keyword(s):  
Colloidal Stability ◽  
Addition Reaction ◽  
Biologically Active ◽  
Metal Nanostructures ◽  
Organic Media ◽  
Metal Oxide Surfaces ◽  
Nucleophilic Addition Reaction ◽  
One Step ◽  
Potential Use ◽  
Polymer Ligand

We designed a new set of polymer ligands that combine multiple metal-coordinating groups and short polyethylene glycol (PEG) moieties in the same structure. The ligand design relies on the controlled grafting of a large number of amine-terminated histamines and PEG short chains onto a poly(isobutylene-alt-maleic anhydride) backbone,viaa one-step nucleophilic addition reaction. This addition reaction is highly efficient, can be carried out in organic media and does not require additional reagents. We show that when imidazole groups are used the resulting polymer ligand can strongly ligate onto metal nanostructures such as nanoparticles (NPs) and nanorods (NRs) made of gold cores. The resulting polymer-coated NPs and NRs exhibit good colloidal stability to pH changes and added electrolytes. This constitutes a departure from the use of thiol-based ligands to coordinate on Au surfaces. The present chemical approach also opens up additional opportunities for designing hydrophilic and reactive platforms where the polymer coating can be adjusted to various metal and metal oxide surfaces by simply modifying or combining the addition reaction with other metal coordinating groups. These could include iron oxide NPs and semiconductor QDs. These polymer-capped NPs and NRs can be used to develop biologically-active platforms with potential use for drug delivery and sensing.

scholarly journals Synthesis of Monodispersed Gold Nanoparticles with Exceptional Colloidal Stability with Grafted Polyethylene Glycol-g-polyvinyl Alcohol

2015 ◽  
Vol 2015 ◽  
pp. 1-9 ◽  
Author(s):  
Alaaldin M. Alkilany ◽  
Alaa I. Bani Yaseen ◽  
Mohammed H. Kailani
Keyword(s):  
Gold Nanoparticles ◽  
Polyethylene Glycol ◽  
Polyvinyl Alcohol ◽  
Colloidal Stability ◽  
Kinetic Mechanism ◽  
Grafted Copolymer ◽  
Synthesis Parameters ◽  
Salt Addition ◽  
One Step ◽  
Spherical Gold Nanoparticles

Herein, we report the synthesis of spherical gold nanoparticles with tunable core size (23–79 nm) in the presence of polyethylene glycol-g-polyvinyl alcohol (PEG-g-PVA) grafted copolymer as a reducing, capping, and stabilizing agent in a one-step protocol. The resulted PEG-g-PVA-capped gold nanoparticles are monodispersed with an exceptional colloidal stability against salt addition, repeated centrifugation, and extensive dialysis. The effect of various synthesis parameters and the kinetic/mechanism of the nanoparticle formation are discussed.

scholarly journals Controlled Formation of a Protein Corona Composed of Denatured BSA on Upconversion Nanoparticles Improves Their Colloidal Stability

Materials ◽  
2021 ◽  
Vol 14 (7) ◽  
pp. 1657
Author(s):  
Samah Shanwar ◽  
Liuen Liang ◽  
Andrey V. Nechaev ◽  
Daria K. Bausheva ◽  
Irina V. Balalaeva ◽  
...  
Keyword(s):  
Colloidal Stability ◽  
Protein Corona ◽  
Systemic Circulation ◽  
Upconversion Nanoparticles ◽  
Long Term Storage ◽  
Theranostic Applications ◽  
Term Storage

In the natural fluidic environment of a biological system, nanoparticles swiftly adsorb plasma proteins on their surface forming a “protein corona”, which profoundly and often adversely affects their residence in the systemic circulation in vivo and their interaction with cells in vitro. It has been recognized that preformation of a protein corona under controlled conditions ameliorates the protein corona effects, including colloidal stability in serum solutions. We report on the investigation of the stabilizing effects of a denatured bovine serum albumin (dBSA) protein corona formed on the surface of upconversion nanoparticles (UCNPs). UCNPs were chosen as a nanoparticle model due to their unique photoluminescent properties suitable for background-free biological imaging and sensing. UCNP surface was modified with nitrosonium tetrafluoroborate (NOBF4) to render it hydrophilic. UCNP-NOBF4 nanoparticles were incubated in dBSA solution to form a dBSA corona followed up by lyophilization. As produced dBSA-UCNP-NOBF4 demonstrated high photoluminescence brightness, sustained colloidal stability after long-term storage and the reduced level of serum protein surface adsorption. These results show promise of dBSA-based nanoparticle pretreatment to improve the amiability to biological environments towards theranostic applications.

scholarly journals Reducing Protein Corona Formation and Enhancing Colloidal Stability of Gold Nanoparticles by Capping with Silica Monolayers

2018 ◽  
Vol 31 (1) ◽  
pp. 57-61 ◽  
Author(s):  
Jesús Mosquera ◽  
Isabel García ◽  
Malou Henriksen-Lacey ◽  
Guillermo González-Rubio ◽  
Luis M. Liz-Marzán
Keyword(s):  
Gold Nanoparticles ◽  
Colloidal Stability ◽  
Protein Corona ◽  
Corona Formation

scholarly journals Revealing the Nanoparticle-Protein Corona with a Solid-State Nanopore

Materials ◽  
2019 ◽  
Vol 12 (21) ◽  
pp. 3524 ◽  
Author(s):  
Diego Coglitore ◽  
Pierre Eugene Coulon ◽  
Jean-Marc Janot ◽  
Sébastien Balme
Keyword(s):  
Gold Nanoparticles ◽  
Solid State ◽  
Protein Adsorption ◽  
Internal Energy ◽  
Colloidal Stability ◽  
Protein Corona ◽  
Intrinsic Properties ◽  
Colloidal Properties ◽  
Salt Addition ◽  
Structural Category

Protein adsorption at the liquid–solid interface is an old but not totally solved topic. One challenge is to find an easy way to characterize the protein behavior on nanoparticles and make a correlation with its intrinsic properties. This work aims to investigate protein adsorption on gold nanoparticles and the colloidal properties. The protein panel was chosen from different structural categories (mainly-α, mainly-β or mix-αβ). The result shows that the colloidal stability with salt addition does not depend on the structural category. Conversely, using the single nanopore technique, we show that the mainly-α proteins form a smaller corona than the mainly-β proteins. We assign these observations to the lower internal energy of α-helices, making them more prone to form a homogeneous corona layer.

Colloidal Stability and Surface Chemistry Are Key Factors for the Composition of the Protein Corona of Inorganic Gold Nanoparticles

2017 ◽  
Vol 27 (42) ◽  
pp. 1701956 ◽  
Author(s):  
Blair D. Johnston ◽  
Wolfgang G. Kreyling ◽  
Christian Pfeiffer ◽  
Martin Schäffler ◽  
Hakan Sarioglu ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Surface Chemistry ◽  
Colloidal Stability ◽  
Protein Corona ◽  
Key Factors

scholarly journals One-Step Synthesis of PEGylated Gold Nanoparticles with Tunable Surface Charge

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
Rares Stiufiuc ◽  
Cristian Iacovita ◽  
Raul Nicoara ◽  
Gabriela Stiufiuc ◽  
Adrian Florea ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Surface Charge ◽  
Colloidal Stability ◽  
Spherical Shape ◽  
Average Diameter ◽  
Molecular Chain ◽  
Surface Charges ◽  
One Step

The present work reports a rapid, simple and efficient one-step synthesis and detailed characterisation of stable aqueous colloids of gold nanoparticles (AuNPs) coated with unmodified poly(ethylene)glycol (PEG) molecules of different molecular weights and surface charges. By mixing and heating aqueous solutions of PEG with variable molecular chain and gold(III) chloride hydrate (HAuCl4) in the presence of NaOH, we have successfully produced uniform colloidal 5 nm PEG coated AuNPs of spherical shape with tunable surface charge and an average diameter of 30 nm within a few minutes. It has been found out that PEGylated AuNPs provide optical enhancement of the characteristic vibrational bands of PEG molecules attached to the gold surface when they are excited with both visible (532 nm) and NIR (785 nm) laser lines. The surface enhanced Raman scattering (SERS) signal does not depend on the length of the PEG molecular chain enveloping the AuNPs, and the stability of the colloid is not affected by the addition of concentrated salt solution (0.1 M NaCl), thus suggesting their potential use forin vitroandin vivoapplications. Moreover, by gradually changing the chain length of the biopolymer, we were able to control nanoparticles’ surface charge from −28 to −2 mV, without any modification of the Raman enhancement properties and of the colloidal stability.

Preparation of Gold Nanoparticles in an Aqueous Medium Using 2-Mercaptosuccinic Acid as Both Reduction and Capping Agent

2008 ◽  
Vol 8 (4) ◽  
pp. 2062-2068 ◽  
Author(s):  
K. Vasilev ◽  
T. Zhu ◽  
G. Glasser ◽  
W. Knoll ◽  
M. Kreiter
Keyword(s):  
Electron Microscopy ◽  
Gold Nanoparticles ◽  
Particle Growth ◽  
Capping Agent ◽  
Mercaptosuccinic Acid ◽  
Vis Spectroscopy ◽  
One Step ◽  
Reaction In Water ◽  
Scanning Electron

Monolayer protected gold nanoparticles with diameters above 10 nm were prepared by a simple, one step reaction in water. 2-mercaptosuccinic acid (MSA) was used both as reduction agent for hydrogen tetrachloroaurate (HAuCl4) and as stabilizing agent for the gold nanoparticles. Size distribution and surface chemistry were investigated by UV-Vis spectroscopy, scanning electron microscopy and Fourier Transform Infrared Spectroscopy. Particle size can be controlled by adjusting the molar portions of the reactants. The resulting particles are efficiently stabilized against aggregation when MSA is used in a concentration of 40% and above. Below a minimum MSA concentration a long-term particle growth is observed.

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