scholarly journals Injectable Hydrogels with In Situ Double Network Formation Enhance Retention of Transplanted Stem Cells

2015 ◽  
Vol 25 (9) ◽  
pp. 1344-1351 ◽  
Author(s):  
Lei Cai ◽  
Ruby E. Dewi ◽  
Sarah C. Heilshorn
Keyword(s):  
Stem Cells ◽  
Network Formation ◽  
Injectable Hydrogels ◽  
Double Network

scholarly journals Regulating Stem Cell Secretome Using Injectable Hydrogels with In Situ Network Formation

2016 ◽  
Vol 5 (21) ◽  
pp. 2758-2764 ◽  
Author(s):  
Lei Cai ◽  
Ruby E. Dewi ◽  
Andrew B. Goldstone ◽  
Jeffrey E. Cohen ◽  
Amanda N. Steele ◽  
...  
Keyword(s):  
Stem Cell ◽  
Network Formation ◽  
Injectable Hydrogels

scholarly journals An in situ Gelling BMSC-laden Collagen/Silk Fibroin Double Network Hydrogel for Cartilage Regeneration

2021 ◽  
Author(s):  
Yajie Zhang ◽  
Min Liu ◽  
Renjun Pei
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Cartilage Tissue Engineering ◽  
Cartilage Regeneration ◽  
Cartilage Tissue ◽  
Injectable Hydrogels ◽  
Double Network ◽  
Good Biocompatibility ◽  
In Situ Gelling

Collagen (Col)-based injectable hydrogels are desirable scaffolds for cartilage tissue engineering. However, fabrication of Col-based injectable hydrogels with short gelation times, good biocompatibility and high mechanical properties still faces great...

Mechanical Properties and Biocompatibility of in Situ Enzymatically Cross-Linked Gelatin Hydrogels

2017 ◽  
Vol 40 (4) ◽  
pp. 159-168 ◽  
Author(s):  
Nada Z. Alarake ◽  
Patrick Frohberg ◽  
Thomas Groth ◽  
Markus Pietzsch
Keyword(s):  
Mechanical Properties ◽  
Stem Cells ◽  
Cell Proliferation ◽  
Cross Linking ◽  
Adipose Derived Stem Cells ◽  
Swelling Ratio ◽  
Connective Tissues ◽  
Injectable Hydrogels ◽  
Cross Linker

Objectives Gelatin, a degraded collagen, has been widely used as a scaffolding material in tissue engineering applications. In this work, we aimed at the development of in situ, cross-linking, cytocompatible hydrogels by the use of transglutaminase as a cross-linker for potential application in the regeneration of tissues. Methods Hydrogels were prepared from gelatin of different concentrations and bloom degree (175 (G175) or 300 (G300) bloom gelatin) and cross-linked with various amounts of microbial transglutaminase (mTG) at 37°C. Mechanical properties and cross-linking degree were studied by rheology and swelling experiments. Four hydrogels with different stiffness were selected for studies with embedded human adipose-derived stem cells (hASCs). Results Hydrogels were obtained with storage modulus (G’) values between 11 (±1) Pa and 1,800 (±200) Pa with gelation times between 80 (±6) and 450 (±36) seconds. G300 cross-linked gelatin hydrogels displayed higher gel stiffness, lower swelling ratio and gelled more rapidly compared to the hydrogels prepared from G175. Stiffer hydrogels (50 and 200 Pa) showed greater ability to support the proliferation of hASCs than softer ones (11 and 30 Pa). The highest cell proliferation was observed with a hydrogel of 200 Pa modulus. Conclusions Overall, transglutaminase cross-linked gelatin hydrogels might be suitable as injectable hydrogels for the engineering of musculoskeletal and other types of connective tissues.

Rapid reprogramming of tumour cells into cancer stem cells on double-network hydrogels

2021 ◽  
Author(s):  
Jun Suzuka ◽  
Masumi Tsuda ◽  
Lei Wang ◽  
Shinji Kohsaka ◽  
Karin Kishida ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cancer Stem Cells ◽  
Tumour Cells ◽  
Double Network

scholarly journals Construction of a mammalian embryo model from stem cells organized by a morphogen signalling centre

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Peng-Fei Xu ◽  
Ricardo Moraes Borges ◽  
Jonathan Fillatre ◽  
Maraysa de Oliveira-Melo ◽  
Tao Cheng ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Tracking ◽  
Cardiac Tissue ◽  
Embryonic Stem ◽  
Mammalian Embryo ◽  
Wide Range ◽  
Vitro Model ◽  
Neurula Stage

AbstractGenerating properly differentiated embryonic structures in vitro from pluripotent stem cells remains a challenge. Here we show that instruction of aggregates of mouse embryonic stem cells with an experimentally engineered morphogen signalling centre, that functions as an organizer, results in the development of embryo-like entities (embryoids). In situ hybridization, immunolabelling, cell tracking and transcriptomic analyses show that these embryoids form the three germ layers through a gastrulation process and that they exhibit a wide range of developmental structures, highly similar to neurula-stage mouse embryos. Embryoids are organized around an axial chordamesoderm, with a dorsal neural plate that displays histological properties similar to the murine embryo neuroepithelium and that folds into a neural tube patterned antero-posteriorly from the posterior midbrain to the tip of the tail. Lateral to the chordamesoderm, embryoids display somitic and intermediate mesoderm, with beating cardiac tissue anteriorly and formation of a vasculature network. Ventrally, embryoids differentiate a primitive gut tube, which is patterned both antero-posteriorly and dorso-ventrally. Altogether, embryoids provide an in vitro model of mammalian embryo that displays extensive development of germ layer derivatives and that promises to be a powerful tool for in vitro studies and disease modelling.

scholarly journals Radiation-initiated high strength chitosan/lithium sulfonate double network hydrogel/aerogel with porosity and stability for efficient CO2 capture

RSC Advances ◽  
2021 ◽  
Vol 11 (33) ◽  
pp. 20486-20497
Author(s):  
Zhiyan Liu ◽  
Rui Ma ◽  
Wenjie Du ◽  
Gang Yang ◽  
Tao Chen
Keyword(s):  
Aqueous Solution ◽  
High Strength ◽  
Beam Radiation ◽  
Chitosan Hydrogel ◽  
Double Network ◽  
Electron Beam Radiation ◽  
Freeze Dried ◽  
Urea Aqueous Solution ◽  
Capture Capacity

Chitosan hydrogel is regenerated from alkali/urea aqueous solution and the lithium sulfonate second network is introduced by electron beam radiation-initiated in situ free radical polymerization. The freeze-dried aerogel has CO2 capture capacity.

Effect of electrical stimulation on chondrogenic differentiation of mesenchymal stem cells cultured in hyaluronic acid – Gelatin injectable hydrogels

2020 ◽  
Vol 134 ◽  
pp. 107536 ◽  
Author(s):  
Juan Jairo Vaca-González ◽  
Sandra Clara-Trujillo ◽  
María Guillot-Ferriols ◽  
Joaquín Ródenas-Rochina ◽  
María J. Sanchis ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Electrical Stimulation ◽  
Hyaluronic Acid ◽  
Chondrogenic Differentiation ◽  
Injectable Hydrogels ◽  
Cells Cultured

The osteogenic differentiation of rat muscle-derived stem cells in vivo within in situ-forming chitosan scaffolds

Biomaterials ◽  
2008 ◽  
Vol 29 (33) ◽  
pp. 4420-4428 ◽  
Author(s):  
Kyung Sook Kim ◽  
Jung Hwa Lee ◽  
Hyun Hee Ahn ◽  
Ju Young Lee ◽  
Gilson Khang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Osteogenic Differentiation ◽  
Rat Muscle ◽  
In Situ Forming ◽  
Chitosan Scaffolds
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