MORC3, a novel MIWI2 association partner, as an epigenetic regulator of piRNA dependent transposon silencing in male germ cells

The PIWI (P-element-induced wimpy testis)-interacting-RNA (piRNA) pathway plays a crucial role in the repression of TE (transposable element) expression via de novo DNA methylation in mouse embryonic male germ cells. Various proteins, including MIWI2 are involved in the process. TE silencing is ensured by piRNA-guided MIWI2 that recruits some effector proteins of the DNA methylation machinery to TE regions. However, the molecular mechanism underlying the methylation is complex and has not been fully elucidated. Here, we identified MORC3 as a novel associating partner of MIWI2 and also a nuclear effector of retrotransposon silencing via piRNA-dependent de novo DNA methylation in embryonic testis. Moreover, we show that MORC3 is important for transcription of piRNA precursors and subsequently affects piRNA production. Thus, we provide the first mechanistic insights into the role of this effector protein in the first stage of piRNA biogenesis in embryonic TE silencing mechanism.

MORC3, a novel MIWI2 association partner, is an epigenetic regulator of piRNA-dependent transposon silencing.

The PIWI (P-element-induced wimpy testis)-interacting-RNA (piRNA) pathway plays a crucial role in the repression of TE (transposable element) expression via de novo DNA methylation in mouse embryonic male germ cells. Various proteins, including MIWI2 are involved in the process. TE silencing is ensured by piRNA-guided MIWI2 that recruits some effector proteins of the DNA methylation machinery to TE regions. However, the molecular mechanism underlying the methylation is complex and has not been fully elucidated. Here, we identified MORC3 as a novel associating partner of MIWI2 and also a nuclear effector of retrotransposon silencing via piRNA-dependent de novo DNA methylation in embryonic testis. Moreover, we show that MORC3 is important for transcription of piRNA precursors and subsequently affects piRNA production. Thus, we provide the first mechanistic insights into the role of this effector protein in the first stage of piRNA biogenesis in embryonic TE silencing mechanism.

Market Opportunities for “More Sustainable Chemistry” Through the REACH Regulation - Results from a Case Study of the Global Supply Chains in the Textile and Sporting Goods Industry

The report outlines the results of the research project ‘Market opportunities for “more sustainable chemistry” through the REACH Regulation (Sustainable Sporting Goods – SuSport)’. The aim of the project completed in 2018 is to support ‘more sustainable chemistry’ in the textile supply chain and to broaden the view from the ‘reactive’ compliance position to a ‘proactive’ – beyond compliance perspective. Strategically, this approach is based on the following consideration: those who still want to be ‘compliant’ tomorrow have to act ‘beyond compliance’ today. Such a strategy not only guarantees legal compliance, but also opens up new market opportunities. Relevant actors participating in the transdisciplinary research project are mainly manufacturers of textile chemicals (organised in Germany in the TEGEWA association, partner in the SuSport project) as well as companies that manufacture and sell textile products, such as clothing and sporting goods (for sporting goods in Germany organised in the BSI, also project partner). However, since the project’s subject addresses a transformation process of the global textile supply chains, all other actors involved in the production processes must also be taken into account.

Effect of Chemical Treatment on Alpaca Fleece Reinforced Polymer Composites

Fiber which is a fortification reinforced additionally manufactured characteristic investigations demonstrate solitary counterfeit strands like glass, carbon and so on., are utilized in fiber supported plastics. despite the fact that have extraordinary explicit quality, their grounds of utilization are self-same constrained inferable from their characteristic more expensive rate of creation. In this association, partner degree examination has been distributed to frame utilization of Alpaca fleece a fiber plentifully available in Bharat. Regular filaments don't appear to be just strong and lightweight however moreover generally the most reduced. Alpaca fleece compounds are created and their mechanical possessions remain assessed. Mechanical properties of Alpaca fleece /polymer and contrasted and glass fiber/amino..