auxotrophic requirement
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Antioxidants ◽  
2020 ◽  
Vol 9 (11) ◽  
pp. 1086 ◽  
Author(s):  
Tobias Horn ◽  
Wolfgang Bettray ◽  
Ulrike Noll ◽  
Felix Krauskopf ◽  
Meng-Ruo Huang ◽  
...  

When cells of garlic (Allium sativum) are disrupted by wounding, they produce the defense substance allicin (diallylthiosulfinate). Allicin is an efficient thiol trap and readily passes through cell membranes into the cytosol, where it behaves as a redox toxin by oxidizing the cellular glutathione (GSH) pool and producing S-allylmercaptoglutathione (GSSA). An N-cyanosulfilimine analogue of allicin (CSA), which was predicted to have similar reactivity towards thiol groups but be more stable in storage, was synthesized and its properties investigated. Similarly to allicin, CSA was shown to inhibit the growth of various bacteria, a fungus (baker’s yeast), and Arabidopsis roots. A chemogenetic screen showed that yeast mutants with compromised GSH levels and metabolism were hypersensitive to CSA. GSH reacted with CSA to produce allyltrisulfanylglutathione (GS3A), which was a white solid virtually insoluble in water. Yeast Δgsh1 mutants are unable to synthesize GSH because they lack the γ-glutamylcysteine synthetase (GSH1) gene, and they are unable to grow without GSH supplementation in the medium. GS3A in the growth medium supported the auxotrophic requirement for GSH in Δgsh1 mutants. This result suggests that GS3A is being reduced to GSH in vivo, possibly by the enzyme glutathione reductase (GR), which has been shown to accept GSSA as a substrate. The results suggest that CSA has a mode of action similar to allicin and is effective at similar concentrations.


2008 ◽  
Vol 190 (17) ◽  
pp. 5907-5914 ◽  
Author(s):  
Arun S. Kharat ◽  
Dalia Denapaite ◽  
Florian Gehre ◽  
Reinhold Brückner ◽  
Waldemar Vollmer ◽  
...  

ABSTRACT The two recently characterized Streptococcus pneumoniae strains—R6Chi and R6Cho−—that have lost the unique auxotrophic requirement of this bacterial species for choline differ in their mechanisms of choline independence. In strain R6Chi the mechanism is caused by a point mutation in tacF, a gene that is part of the pneumococcal lic2 operon, which is essential for growth and survival of the bacteria. Cultures of lic2 mutants of the encapsulated strain D39Chi growing in choline-containing medium formed long chains, did not autolyze, had no choline in their cell wall, and were completely avirulent in the mouse intraperitoneal model. In contrast, while the Cho− strain carried a complete pneumococcal lic2 operon and had no mutations in the tacF gene, deletion of the entire lic2 operon had no effect on the growth or phenotype of strain Cho−. These observations suggest that the biochemical functions normally dependent on determinants of the pneumococcal lic2 operon may also be carried out in strain Cho− by a second set of genetic elements imported from Streptococcus oralis, the choline-independent streptococcal strain that served as the DNA donor in the heterologous transformation event that produced strain R6Cho−. The identification in R6Cho− of a large (20-kb) S. oralis DNA insert carrying both tacF and licD genes confirms this prediction and suggests that these heterologous elements may represent a “backup” system capable of catalyzing P-choline incorporation and export of teichoic acid chains under conditions in which the native lic2 operon is not functional.


2003 ◽  
Vol 185 (19) ◽  
pp. 5831-5837 ◽  
Author(s):  
Juan C. Tantaleán ◽  
Manuel A. Araya ◽  
Claudia P. Saavedra ◽  
Derie E. Fuentes ◽  
José M. Pérez ◽  
...  

ABSTRACT Many eubacteria are resistant to the toxic oxidizing agent potassium tellurite, and tellurite resistance involves diverse biochemical mechanisms. Expression of the iscS gene from Geobacillus stearothermophilus V, which is naturally resistant to tellurite, confers tellurite resistance in Escherichia coli K-12, which is naturally sensitive to tellurite. The G. stearothermophilus iscS gene encodes a cysteine desulfurase. A site-directed mutation in iscS that prevents binding of its pyridoxal phosphate cofactor abolishes both enzyme activity and its ability to confer tellurite resistance in E. coli. Expression of the G. stearothermophilus iscS gene confers tellurite resistance in tellurite-hypersensitive E. coli iscS and sodA sodB mutants (deficient in superoxide dismutase) and complements the auxotrophic requirement of an E. coli iscS mutant for thiamine but not for nicotinic acid. These and other results support the hypothesis that the reduction of tellurite generates superoxide anions and that the primary targets of superoxide damage in E. coli are enzymes with iron-sulfur clusters.


2003 ◽  
Vol 185 (1) ◽  
pp. 311-316 ◽  
Author(s):  
J. D. Woodson ◽  
R. F. Peck ◽  
M. P. Krebs ◽  
J. C. Escalante-Semerena

ABSTRACT Genetic and nutritional analyses of mutants of the extremely halophilic archaeon Halobacterium sp. strain NRC-1 showed that open reading frame (ORF) Vng1581C encodes a protein with nucleoside triphosphate:adenosylcobinamide-phosphate nucleotidyltransferase enzyme activity. This activity was previously associated with the cobY gene of the methanogenic archaeon Methanobacterium thermoautotrophicum strain ΔH, but no evidence was obtained to demonstrate the direct involvement of this protein in cobamide biosynthesis in archaea. Computer analysis of the Halobacterium sp. strain NRC-1 ORF Vng1581C gene and the cobY gene of M. thermoautotrophicum strain ΔH showed the primary amino acid sequence of the proteins encoded by these two genes to be 35% identical and 48% similar. A strain of Halobacterium sp. strain NRC-1 carrying a null allele of the cobY gene was auxotrophic for cobinamide-GDP, a known intermediate of the late steps of cobamide biosynthesis. The auxotrophic requirement for cobinamide-GDP was corrected when a wild-type allele of cobY was introduced into the mutant strain, demonstrating that the lack of cobY function was solely responsible for the observed block in cobamide biosynthesis in this archaeon. The data also show that Halobacterium sp. strain NRC-1 possesses a high-affinity transport system for corrinoids and that this archaeon can synthesize cobamides de novo under aerobic growth conditions. To the best of our knowledge this is the first genetic and nutritional analysis of cobalamin biosynthetic mutants in archaea.


Genetics ◽  
1988 ◽  
Vol 120 (4) ◽  
pp. 959-964
Author(s):  
C E Bronner ◽  
D L Welker ◽  
R A Deering

Abstract The tmpA600 mutation confers thymidylate synthase deficiency and thymidine auxotrophy to Dictyostelium discoideum. The tdrA600 mutation enhances transport of thymidine and thereby reduces the auxotrophic requirement of tmpA600 strains. The tmpA locus maps to linkage group III. The tdrA600 mutation is dominant and cosegregates with both linkage groups IV and VI, possibly because of a translocation between the two. The tdrA600 allele is sufficient to allow efficient incorporation of exogenous [3H]thymidine or [3H]uridine into TCA-precipitable material and to sensitize the cell to the nucleoside-analog inhibitor, 5-fluorodeoxyuridine. These properties make the tdrA mutation useful for studies requiring labelling of DNA or RNA in vivo.


1986 ◽  
Vol 32 (11) ◽  
pp. 842-846 ◽  
Author(s):  
D. Ramotar ◽  
E. B. Newman

We have shown that serine–glycine auxotrophs of Escherichia coli K-12 use exogenous L-serine inefficiently as a source of biosynthetic intermediates. Much of the L-serine supplied in the medium is not used to satisfy the auxotrophic requirement, owing to its diversion by L-serine deaminase, presumably to pyruvate. This is the first proof that the activity known as L-serine deaminase actually deaminates L-serine in vivo.


1970 ◽  
Vol 15 (1) ◽  
pp. 135-137 ◽  
Author(s):  
J. B. Land ◽  
G. Norton

SUMMARYContrary to previous reports, evidence has been presented which indicates that Xan-b61, a Xantha mutant of barley, does not have an auxotrophic requirement for leucine. It has been shown that this mutant, when grown under appropriate conditions in the absence of leucine, will synthesize leucine and will continue to grow for an extended period of time.


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