A New Approach to Harness Probiotics Against Common Bacterial Skin Pathogens: Towards Living Antimicrobials

In this study, the potential of certain lactic acid bacteria—classified as probiotics and known to be antimicrobially active against pathogens or food-poisoning microorganisms—was evaluated with respect to their activity against bacterial skin pathogens. The aim of the study was to develop a plaster/bandage for the application of inhibitory substances produced by these probiotics when applied to diseased skin. For this purpose, two Streptococcus salivarius strains and one Lactobacillus plantarum were tested for production of antimicrobials (bacteriocin-like substances) active against Gram-positive and Gram-negative pathogens using established methods. A newly designed membrane test ensured that the probiotics produce antimicrobials diffusible through membranes. Target organisms used were Cutibacterium acnes, Staphylococcus aureus, and Pseudomonas aeruginosa. Moreover, the L. plantarum 8P-A3 strain was tested against additional bacteria involved in skin disorders. The Lactobacillales used were active against all potential skin pathogens tested. These probiotics could be enclosed between polymer membranes—one tight, the other permeable for their products, preserved by vacuum drying, and reactivated after at least three months storage. Importantly, the reactivated pads containing the probiotics demonstrated antibacterial activity on agar plates against all pathogens tested. This suggests that the probiotic containing pads may be topically applied for the treatment of skin disorders without the need for a regular antibiotic treatment or as an adjunctive therapy.

A Study On Work-Life Balance In Working Women

The purpose of working women has changed throughout the creation due to economic shifts and social needs. This has resulted in a scenario in which working women have tremendous pressure to develop a career as robust as their male counterparts while nourishing active engagement in personal life. The ever-increasing workload is bringing a plaster bandage on the working women leaving them with less time for themselves. The increasing responsibilities on the personal front with the technological blessings like advanced mobile phones, notepads, etc. that keeps work life integrated with personal life also creates stress on personal and professional fronts in this knowledge age

Influence of osteopathic correction on the terms of adhesion of fractures of the distal metaepiphysis of the radial bone

Introduction. In 26-43 % of fractures of the bones of upper extremities secondary displacement of fragments occurs in the plaster bandage. It is probable that osteopathic treatment of patients with bone injuries of upper extremities promotes more rapid recovery of impaired functions of the damaged structure preventing possible complications and disability and improving patients' quality of life. That is why it is viable to study the infl uence of osteopathic correction on the healing process of the Colles’ fracture with displacement of bone fragments.Goal of research - to evaluate the effect of osteopathic treatment on the process of adhesion of Colles’ fracture with the displacement of fragments.Materials and methods. 40 patients took part in the research. 20 of them formed the main group. The other 20 formed the control group. An osteopathic examination of patients was performed and somatic dysfunctions were found. The visual analogue scale of pain was used. In order to evaluate the terms of adhesion, an X-ray diagnostic method was used.Results. Osteopathic diagnostics showed that somatic dysfunctions of the left or right wrist were dominant in patients admitted to the trauma bay with Colles’ fracture. Patients of the main group showed a faster normalization of the microcirculation, which meant that negative consequences of edema were less likely to appear.Conclusion. The study of the effect of osteopathic treatment of fractures of distal metaepiphysis of radial bone should be continued. The focus should be on the development and use of new diagnostic methods, which can prove the positive effect of osteopathic treatment on the terms of adhesion of the radial bone.

Clinical case for the use of intramedullary osteosynthesis in the treatment of pathological fractures of the femur in 6-day newborn girls with a high partial intestinal obstruction

Skeletal bone fractures in newborns present a problem of modern traumatology. Aim. The goal is to present the use of the method of intramedullary osteosynthesis in the treatment of a pathological fracture of the right femur in a newborn girl to a wide audience of pediatric orthopedic traumatologists. Material and methods. We have experience in the treatment of a 6-day-old girl, who was born with intrauterine growth retardation and bowel disease. On the second day of stay in the intensive care unit, she had a pathological fracture of the right femur. Diagnosis of the pathological fracture was established based upon the results of clinical examination and radiography of the injured limb segment. Results. Immediately after the diagnosis, the right lower limb of the child was fixed with a plaster bandage. On the control radiographs, the standing of the bone fragments were unsatisfactory, and a decision was made to use the intramedullary osteosynthesis method with a knitting needle on the 6th day after birth of the child. The need for surgical treatment of a fracture of the femur was due to a congenital abnormality of the intestine in the child, and a need to perform surgery on the abdominal organs. Discussion. Spontaneous fracture of the right femur occurred in the child in treatment in the intensive care unit. The cause of the fracture was osteopenic syndrome, which developed as a result of vitamin D deficiency. During the first 12 days of her life, the newborn had two laparoscopic operations to address the intestinal pathology. Four weeks after the operation using osteosynthesis, the metal from the bone marrow channel of the right femur was removed. After the removal of the needle, the correct axis of the operated segment was fixed to the same length of the lower extremities. The patient had absence of pathological mobility in the fracture region, and full amplitude of movements in the knee and hip joints. Conclusion. When receiving fractures of the femur, both traditionally used conservative methods of treatment, as well as surgical methods can be used on newborn children. This is especially true, when a newborn has an accompanying congenital pathology of the internal organs requiring immediate treatment.

Dilatancy-based impression and fabrication technique for custom foot orthoses

Background and aim: Current methods used to take impressions for custom foot orthoses include plaster bandage, foam box, fiberglass, and laser-optical scanner. Impressions are converted to positive plaster or foam models. These methods create waste and may not be feasible in resource-limited areas. This technical note presents an alternative, greener impression and fabrication technique for foot orthoses that utilizes the dilatancy principle. Technique: Steps of the dilatancy (vacuum-based) procedure include taking an impression of the foot, converting the negative mold to a positive sand model, modifying the positive sand model, and thermoforming the foot orthosis. Discussion: This plaster-less system is inexpensive to set up and maintain, is reusable thereby minimizing cost and waste, and is clean to use. It enables a practitioner to quickly take an impression for fabricating a foot orthosis in a short period of time during a single clinic visit by the patient. Clinical relevance The dilatancy casting system could potentially be a cheaper, faster, and greener alternative procedure for fabricating custom foot orthoses in both developing and developed countries.

Our Experience in Treatment of Patients with Patella Dislocation

The experience in surgical treatment of 33 patients with patella dislocation of various etiology and severity degree (38 dislocations) has been summarized. The methods of preoperative exam­ination and indications for the application of various treatment methods has been described. Two methods of patella dislocation elimination were used, i.e. myofascioplasty and reconstruc­tion of the knee joint extension apparatus with transposition of the patellar ligament medially. When indicated those methods were combined with correcting osteotomy, closed elimination of knee joint flexion contracture and shin subluxation using Ilizarov device. Postoperative fixation was performed with plaster bandage and various arrangments of Ilizarov apparatus. In the second case the possibility of early knee joint movements was provided and markedly faster restoration of joint functions was noted. Good results were achieved in 36cases, satisfactory results — in 2 cases.

Mistakes and Complications in Conservative Treatmen of Congenital Hip Dislocation in Children

Retrospective analysis of treatment of 183 patients, aged 1 month-18 months, with congenital hip dislocation was performed. 146 patients had failed hip reduction. It was detected that in 134 cases (91.8%) inadequate treatment and/or wrong management and behavior of parents took place. Failed treatment with Frejka pillow, abductive splint, Pavlic device, functional plaster bandage, closed reduction were considered. Main mistakes in application of those methods as well as violation of treatment tactics were described. It was shown that repeated failed femoral head reductions were unfavorable factors for the following development of hip joint.