Cytogenetical analysis of Clitoria ternatea

Clitoria ternatea L. of the family Fabaceae is an economical, ornamental as well as medicinal important species. Chromosome characterization of C. ternatea, encompassing karyomorphological as well as fluorochrome binding was carried out in the present investigation. Karyomorphological studies showed the presence of 2n = 16 somatic chromosome number with three pairs of metacentric chromosomes and five pairs of submedian chromosomes. The analysis also revealed the present of one pair of nucleolar organizing region or satellite. Fluorochrome binding using chromomycin A3 (CMA) and 4-6-diamidino-2-phenylindole (DAPI) showed the presence of GC- and AT- rich heterochromatic region. Further analysis revealed the percentage of GC-rich was comparatively higher than AT-rich heterochromatic region. This is the first report on heterochromatin characterization in C. ternatea.

Cytogenetical analysis of 12 taxa of Genista L. (Fabaceae) from Turkey

In this study, cytogenetical analyses of 12 taxa belonging to the genus Genista L. and grown naturally in Turkey were conducted. These taxa include G. acanthoclada, G. albida, G. anatolica, G. aucheri, G. burdurensis, G. carinalis, G. involucrata, G. januensis subsp. lydia, G. sandrasica, G. sessilifolia, G. tinctoria and G. vuralii. Chromosome numbers of all the taxa, except G. tinctoria, are introduced to the scientific community for the first time. Somatic metaphase chromosomes of the genus were determined as 2n = 18, 36, 46, 48, 52, 72 and 144. Polyploidy was observed in the cells of G. albida (2n = 18, 2n = 4x = 36), G. tinctoria (2n = 36, 48, 2n = 4x = 96), G. januensis subsp. lydia (2n = 46, 2n = 4x = 92), G. burdurensis and G. sessilifolia (2n = 48, 2n = 4x = 96). Somatic chromosomes of the G. sandrasica are very small, thus exact ploidy level of polyploidy was not determined. Karyotype analysis of G. albida and G. involucrata were performed via an Image Analysis System. Keywords: Genista; Leguminosae; chromosome number; karyotype; Turkey. DOI: 10.3329/bjpt.v16i2.3927 Bangladesh J. Plant Taxon. 16(2): 151-156, 2009 (December)