6b gene
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2015 ◽  
Vol 28 (5) ◽  
pp. 580-589 ◽  
Author(s):  
M. Dequivre ◽  
B. Diel ◽  
C. Villard ◽  
O. Sismeiro ◽  
M. Durot ◽  
...  

Novel ways of regulating Ti plasmid functions were investigated by studying small RNAs (sRNAs) that are known to act as posttranscriptional regulators in plant pathogenic bacteria. sRNA-seq analyses of Agrobacterium fabrum C58 allowed us to identify 1,108 small transcripts expressed in several growth conditions that could be sRNAs. A quarter of them were confirmed by bioinformatics or by biological experiments. Antisense RNAs represent 24% of the candidates and they are over-represented on the pTi (with 62% of pTi sRNAs), suggesting differences in the regulatory mechanisms between the essential and accessory replicons. Moreover, a large number of these pTi antisense RNAs are transcribed opposite to those genes involved in virulence. Others are 5′- and 3′-untranslated region RNAs and trans-encoded RNAs. We have validated, by rapid amplification of cDNA ends polymerase chain reaction, the transcription of 14 trans-encoded RNAs, among which RNA1111 is expressed from the pTiC58. Its deletion decreased the aggressiveness of A. fabrum C58 on tomatoes, tobaccos, and kalanchoe, suggesting that this sRNA activates virulence. The identification of its putative target mRNAs (6b gene, virC2, virD3, and traA) suggests that this sRNA may coordinate two of the major pTi functions, the infection of plants and its dissemination among bacteria.


Planta ◽  
2013 ◽  
Vol 238 (4) ◽  
pp. 753-770 ◽  
Author(s):  
Sachiko Takahashi ◽  
Rui Sato ◽  
Miho Takahashi ◽  
Noriko Hashiba ◽  
Atsushi Ogawa ◽  
...  

2012 ◽  
Vol 84 (9) ◽  
pp. 1388-1395 ◽  
Author(s):  
Masaru Ihira ◽  
Yoshihiko Enomoto ◽  
Yoshiki Kawamura ◽  
Hidetaka Nakai ◽  
Ken Sugata ◽  
...  
Keyword(s):  
Rt Pcr ◽  

Author(s):  
Hossam Murad ◽  
Philippe Collet ◽  
Cecile Huin-Schohn ◽  
Nehmann Al-Makdissy ◽  
Geraldine Kerjan ◽  
...  

Planta ◽  
2005 ◽  
Vol 223 (2) ◽  
pp. 237-247 ◽  
Author(s):  
Yasutaka Kakiuchi ◽  
Ivan Gàlis ◽  
Shigeru Tamogami ◽  
Hiroetsu Wabiko

Genomics ◽  
2004 ◽  
Vol 83 (6) ◽  
pp. 1141-1150 ◽  
Author(s):  
Philippe Collet ◽  
Lionel Domenjoud ◽  
Marie Dominique Devignes ◽  
Hossam Murad ◽  
Hervé Schohn ◽  
...  
Keyword(s):  

2004 ◽  
Vol 65 (2) ◽  
pp. 169-179 ◽  
Author(s):  
Ivan Gális ◽  
Yasutaka Kakiuchi ◽  
Petr Šimek ◽  
Hiroetsu Wabiko

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