Evaluation of Dexamethasone-Induced Osteoporosis In Vivo Using Zebrafish Scales

Glucocorticoid-induced osteoporosis (GIOP) is a major cause of secondary osteoporosis, and the pathogenic mechanisms of GIOP remain to be elucidated. Here, we show a rapid dexamethasone-induced osteoporosis animal model using zebrafish scales. Intraperitoneal injection of dexamethasone over a 5-day period suppressed the regeneration of scales. Furthermore, the circularity of the newly formed regenerated scales was also slightly reduced compared to that of the control group on day 5. The changes in bone-related enzymes, such as cathepsin K, tartrate-resistant acid phosphatase (TRAP) for bone resorption, and alkaline phosphatase (ALP) for bone formation, provide insight into the progression of bone diseases; therefore, we further developed a method to measure the activities of cathepsin K, TRAP, and ALP using zebrafish scales. We found that a lysis buffer with detergent at neutral pH under sonication efficiently helped extract these three enzymes with high activity levels. Interestingly, treatment with a dexamethasone injection produced considerably higher levels of cathepsin K activity and a lower Ca/P ratio than those in the control group, suggesting that dexamethasone increased osteoclast activity, with no significant changes in the activities of TRAP and ALP. Our GIOP model and enzyme assay method could help to design better treatments for GIOP.

The naturally occurring radioactivity of ‘scalar energy’ pendants and concomitant radiation risk

Forming part of a study of radiological risk arising from use of radioactive consumer products, investigation is made of pendants containing naturally occurring radioactive material. Based on use of gamma-ray spectrometry and Monte Carlo (MC) simulations, the study investigates commercially available ‘scalar energy pendants’. The doses from these have been simulated using MIRD5 mathematical phantoms, evaluation being made of dose conversion factors (DCFs) and organ dose. Metallic pendants code MP15 were found to contain the greatest activity, at 7043 ± 471 Bq from 232Th, while glass pendants code GP11 were presented the greatest 238U and 40K activity, at 1001 ± 172 and 687 ± 130 Bq respectively. MP15 pendants offered the greatest percentage concentrations of Th, Ce, U and Zr, with means of 25.6 ± 0.06, 5.6 ± 0.005, 1.03 ± 0.04 and 28.5 ± 0.08 respectively, giving rise to an effective dose of 2.8 mSv for a nominal wearing period of 2000 h. Accordingly, these products can give rise to annual doses in excess of the public limit of 1 mSv.

The effects of different cooking modes on the 137Cs, 40K, and total K content in Boletus edulis (King Bolete) mushrooms

This study aimed to get an insight into the effects of household processing on the leaching behaviour of 137Cs and 40K from fresh, frozen and dried Boletus edulis (King Bolete) mushrooms. Three processes were investigated—blanching, blanching and pickling, and drying followed by grinding and soaking. The activity concentrations of 137Cs and 40K in the fresh unprocessed mushrooms were 270 Bq kg−1 dry biomass (27 Bq kg−1 whole weight) and 590 Bq kg−1 db (59 Bq kg−1 ww), respectively. Blanching of fresh mushrooms decreased 137Cs activity by 55%, and 40K activity by 34%, and blanching of deep-frozen mushrooms caused a reduction of 52% and 44% (db) (equivalent to whole weight reductions of 37% and 8.5%, and 67% and 22%, respectively). Blanching and pickling of fresh mushrooms decreased 137Cs activity by 83% and 40K activity by 87%, while blanching deep-frozen mushrooms resulted in decreases of 88% and 80% (db) (whole weight decreases of 77% and 81%, and by ~ 84% and 72%, respectively). This study confirms earlier reports that blanching of fresh or frozen mushrooms alone is not as efficient at removing 137Cs as blanching followed by pickling. The study also shows that the initial rate of fruiting body disintegration and pre-preparation (comparing fresh, deep-frozen, or dried and ground) can have an impact on the leaching rate of the water soluble fraction of metallic elements.

Fluid Infiltration and Mass Transfer along a Lamprophyre Dyke–Marble Contact: An Example from the South-Western Korean Peninsula

In this contribution, we report the metasomatic characteristics of a lamprophyre dyke–marble contact zone from the Hongseong–Imjingang belt along the western Gyeonggi Massif, South Korea. The lamprophyre dyke intruded into the dolomitic marble, forming a serpentinized contact zone. The zone consists of olivine, serpentine, calcite, dolomite, biotite, spinel, and hematite. Minor F and Cl contents in the serpentine and biotite indicate the composition of the infiltrating H2O-CO2 fluid. SiO2 (12.42 wt %), FeO (1.83 wt %), K2O (0.03 wt %), Sr (89 ppm), U (0.7 ppm), Th (1.44 ppm), and rare earth elements (REEs) are highly mobile, while Zr, Cr, and Ba are moderately mobile in the fluid. Phase equilibria modelling suggests that the olivine, spinel, biotite, and calcite assemblage might be formed by the dissolution of dolomite at ~700 °C, 130 MPa. Such modelling requires stable diopside in the observed conditions in the presence of silica-saturated fluid. The lack of diopside in the metasomatized region is due to the high K activity of the fluid. Our log activity K2O (aK2O)–temperature pseudosection shows that at aK2O~−40, the olivine, spinel, biotite, and calcite assemblage is stable without diopside. Subsequently, at ~450 °C, 130 MPa, serpentine is formed due to the infiltration of H2O during the cooling of the lamprophyre dyke. This suggests that hot H2O-CO2 fluids with dissolved major and trace elements infiltrated through fractures, grain boundaries, and micron-scale porosity, which dissolved dolomite in the marble and precipitated the observed olivine-bearing peak metasomatic assemblage. During cooling, exsolved CO2 could increase the water activity to stabilize the serpentine. Our example implies that dissolution-reprecipitation is an important process, locally and regionally, that could impart important textural and geochemical variations in metasomatized rocks.

The correlation between photometric variability and radial velocity jitter

Context. Characterizing the relation between stellar photometric variability and radial velocity (RV) jitter can help us to better understand the physics behind these phenomena. The current and upcoming high precision photometric surveys such as TESS, CHEOPS, and PLATO will provide the community with thousands of new exoplanet candidates. As a consequence, the presence of such a correlation is crucial in selecting the targets with the lowest RV jitter for efficient RV follow-up of exoplanetary candidates. Studies of this type are also crucial to design optimized observational strategies to mitigate RV jitter when searching for Earth-mass exoplanets. Aims. Our goal is to assess the correlation between high-precision photometric variability measurements and high-precision RV jitter over different time scales. Methods. We analyze 171 G, K, and M stars with available TESS high precision photometric time-series and HARPS precise RVs. We derived the stellar parameters for the stars in our sample and measured the RV jitter and photometric variability. We also estimated chromospheric Ca II H & K activity indicator log(RHK′), v sin i, and the stellar rotational period. Finally, we evaluate how different stellar parameters and an RV sampling subset can have an impact on the potential correlations. Results. We find a varying correlation between the photometric variability and RV jitter as function of time intervals between the TESS photometric observation and HARPS RV. As the time intervals of the observations considered for the analysis increases, the correlation value and significance becomes smaller and weaker, to the point that it becomes negligible. We also find that for stars with a photometric variability above 6.5 ppt the correlation is significantly stronger. We show that such a result can be due to the transition between the spot-dominated and the faculae-dominated regime. We quantified the correlations and updated the relationship between chromospheric Ca II H & K activity indicator log(RHK′) and RV jitter.