Calculation of the throwing angle of a fertilizer centrifugal device as functions of random coordinates of feeding point

Liquid fertilizers fed into centrifugal device are spread along angle and radius of feed under action of blades. This article describes how to calculate throwing angular characteristics using Mathcad. The package consists of four programs. Program Mf is intended for calculation of probability density of supply point coordinates under assumption of bivariant normal distribution of system r, γ, which are specified in the form of vectors. The result of the calculation is displayed as matrix Mf. The program Mα calculates the throwing angle for all combinations r, γ.. To calculate the throwing angle, the method of solving differential equations of particle movement along the blade of the device with input data was used: Radius of the disk R, angular speed ω, coefficient of friction of fertilizers on the blade f. The program Ms extracts from the matrix Mf elements Corresponding to a throw angle less than a given number A. The program F (A) sums the elements of the matrix Ms. We obtained the values of the throw angle distribution function by multiplying the resulting sum by the intervals of vectors r and γ. The calculated throwing angle distribution function is approximated by the standard normal distribution function.

Green extraction of healthy and additive free mitochondria with a conventional centrifuge

In this research, we propose a novel centrifugal device for the massive extraction of healthy mitochondria with a centrifuge used in general laboratories within 30 minutes.

Simulation of Fluid Flow in Centrifugal Tricanter

An ANSYS simulation of the multiphase complex fluid flow motion in a centrifugal device (tricanter) is presented in the paper. This centrifugal device is designed for one step efficient solution for contaminated river water processing with oil and oil products. The proposed tricanter is one of the main objectives of the project named “Common strategy to prevent the Danube’s pollution technological risks with oil and oil products CLEANDANUBE” financed by European Commission within the frame of Romania-Bulgaria Trans-Border Cooperation Program 2007 - 2013 (grant MIS-ETC code 653). Results for liquid phases (water and oil products) and for solid particles motion are presented graphically and are commented.

EVALUATION OF HOMOCYSTEINE BINDING WITH A FRACTION OF PLASMA PROTEINS ASSOCIATED WITH VASCULAR REMODELING

Elevated levels of homocysteine (Hcy) in tissues cause cytotoxic effects. Increase in the overall conсentration of the various Hcy chemical forms above 12 μM is called hyperhomocysteinemia. The term «total homocysteine» of plasma (tHcy) is the sum of concentrations of aminothiol in reduced (-SH) and oxidized (-SS-) forms. It was suggested that the S- and, partially, N-homocysteinylation of proteins plays a major role in the toxic effects of Hcy. The rate of tHcy in plasma is not closely related to pathological process with remodeling of the vascular wall and not entirely suitable for prospective evaluation of the pathologic process. The paper presents the method of evaluation of the tHcy and its fraction after ultrafiltration, allowing cut off proteins with mass above 300 kDa. Proteins in this fraction which can form a mixing disulfides of Hcy include apoB protein and activated alpha-2-macroglobulin. The proposed Hcy estimation in plasma preparations prior and after ultrafiltration may be performed in any laboratory with kits for tHcy evaluation in plasma. Evaluation of Hcy fraction associated with > 300 kDa plasma proteins by means of centrifugal device Vivaspin 300 000 MWCO PES «Sartorius» provides information about Hcy transport by an additional way in macrophages, endothelial cells, smooth muscle cells and others in vascular wall by receptorial endocytosis.

Simple Reversed-Phase HPLC Method with Spectrophotometric Detection for Measuring Acetaminophen-Protein Adducts in Rat Liver Samples

A simple reversed-phase HPLC method for measuring hepatic levels of acetaminophen- (APAP-) protein adduct following an overdose of APAP was developed. An aliquot of liver homogenate in phosphate-buffered saline pH 7.4 (PBS) was placed on a Nanosep centrifugal device, which was centrifuged to obtain a protein residue. This residue was incubated with a solution ofp-aminobenzoic acid (PABA), the internal standard, and bacterial protease in PBS, transferred to a Nanosep centrifugal device, and centrifuged. A 100 μL portion of the filtrate was analyzed on a YMC-Pack ODS-AMQ C18 column, using 100 mM potassium dihydrogen phosphate-methanol-acetic acid (100 : 0.6 : 0.1) as the mobile phase, a flow rate of 1 mL/min, and photometric detection at 254 nm. PABA and APAP-cystein-S-yl (APAP-Cys) eluted at~14.7 min and 22.7 min, respectively. Method linearity, based on on-column concentrations of APAP-Cys, was observed over the range 0.078–40 μg. Recoveries of APAP-Cys from spiked blank liver homogenates ranged from~83% to 91%. Limits of detection and of quantification of APAP-Cys, based on column concentrations, were 0.06 μg and 0.14 μg, respectively. RSD values for interday and intraday analyses of a blank liver homogenate spiked with APAP-Cyst at three levels were, in all cases, ≤1.0% and <1.5%, respectively. The proposed method was found appropriate for comparing the antidotal properties of N-acetylcysteine and taurine in a rat model of APAP poisoning.