Giving preparative thin layer chromatography some tender loving care

Preparative thin layer chromatography (prepTLC) is a commonly used method of purification suitable for small scale reactions. However, descriptions of the preferred methodology to load, run, and recover samples from prepTLC are non-standard and varied, making it part of the “hidden curriculum” of laboratory technique. In this article we report on the simple, cost-effective methods we use to load and collect samples from a plate, which enhance the convenience, speed, and precision of this technique.

Covid Conversations 4: Stacy Klein

The ecology of the rural setting in which Double Edge Theatre lives and works is as integral to its artistic work as to its principles of social justice, and these qualities mark the ensemble’s singular profile not only in the United States but also increasingly on the world theatre map. Stacy Klein co-founded the company in Boston in 1982 as a women’s theatre with a defined feminist programme. In 1997, Double Edge moved its work space to a farm that Klein had bought in Ashfield, Massachusetts, commuting from there back to Boston to show its productions. Within a few years, Klein and her collaborators were acutely aware of their separation from the local community, which necessitated a change of perspective to encompass personal and creative engagement with local people and to develop audiences within the area, while not losing sight of their international links. Carlos Uriona, formerly a popular-theatre activist from Argentina, had joined Double Edge and facilitated the local immersion that ultimately became its lifeline, most visibly during the Covid-19 pandemic, as Klein here observes. Klein, who had been a student of Rena Mirecka in Poland (starting in 1976), has maintained her friendship and professional relations with this founding member of the Teatr Laboratorium led by Jerzy Grotowski, inviting Mirecka to run wokshops at the Double Edge Farm. Collaboration with Gardzienice (also from the Grotowski crucible) through the Consortium of Theatre Practices (1999–2001) extended Klein’s Polish connections. She expanded her research on community cultures in Eastern and Central Europe and developed these experiences in her probing, distinctly imaginative explorations of theatre-making, while taking a new approach to participatory theatre-making in Ashfield. Her highly visual and sensual compositions are driven by her sense of the fantastic, no more strikingly so than in Klein’s Summers Spectacles, which are performed outdoors, in concert with the Farm’s natural environment – fields, trees, water, birds, animals, and heaven’s firmament. Double Edge’s profound commitment in the past decade to what it now terms ‘living culture’ and ‘art justice’ has taken root in multiracial collaborations, primarily with the indigenous peoples of Western Massachusetts. This Conversation took place on the winter solstice, 21 December 2020, a date that Maria Shevtsova, Editor of NTQ, had chosen symbolically. It was transcribed by Kunsang Kelden and edited by Shevtsova. Many thanks are extended to Travis Coe of Double Edge for assembling with such loving care the photographs requested.

The Emotional Politics of Limerence in Romantic Comedy Films

“Limerence” describes the intensity of emotions often felt during the pair-forming stage of a romantic relationship, a period that is also the primary focus of many romantic comedy films. This chapter asks how filmmakers have used depictions of limerence to highlight spaces in which its potential for both disruption and loving care could be brought to political spheres. I look at a series of millennial romantic comedies that express emotional upheaval, vulnerability, and openness to change as qualities of relevance to both a romantic and a political selfhood. These “political romcoms” reveal a range of dynamic relations between notions of character competence, moral fiber, personality, and deservedness, and invite investigation of complex emotions that modify a more generalized positive affect associated with romantic comedy cinema: humiliation as a comic device and the existential fear of rejection.

Kirby's Rule of 20: the veterinary nurse's critical patient checklist part 4

Kirby's Rule of 20 is a patient checklist including 20 parameters that should be checked daily in the critically ill patient. It reviews the established evidence-based information regarding patient checklist use in veterinary emergency and critical care medicine. The list of 20 will be discussed over a four-part series to give an appropriate level of information and attention to each patient parameter. Part 4 includes: wound healing, drug dosage and metabolism, pain control, nursing care, tender loving care.

INTEGRATIVE VALUES OF SUFISM AND NATIONALISM WITHIN SANTRI COMMUNITIES AT PONDOK PESANTREN DAARUL ULUM WAL ḤIKAM YOGYAKARTA (LEADERSHIP AND CHARACTER BUILDING ANALYSIS)

<span class="fontstyle0">National identity crisis emerged in the country; many pesantren understand Sufism textually, and the need to dialogue Sufism in the national context. The Pesantren of Pesantren Daarul Ulum wal </span><span class="fontstyle2">Ḥ</span><span class="fontstyle0">ikam Yogyakarta prepared future leaders with high moral values through the implementation of Sufism and nationalism education in an implementative way through a humanist approach. The researcher employs qualitative method for the field research. Data collection techniques are observation, interview anddocumentation. The data gained then is analyzed through data reduction, data presentation<br />and conclusion making. The validity test of the research data used triangulation techniques and sources. The results showed that, Sufism values are implanted at boarding Daarul Ulumwal Hikam through several methods: (1) role models, (2) spiritual training with phases of Takhalli, Tahalli, Tajalli, and (3) story telling. The values of Sufism include: sincerity,<br />repentance, khauf and raja', zuhud, faqir, patience, ridha, muraqabah. The sufism at thispesantren followed Sunni Sufism based on Ahlussunah wal Jama'ah. Nationalism valuesare inculcated through the following methods: (1) knowing the good, (2) loving the good ,(3) desiring the good. Nationalism values include religious, tolerance, hard work, independent, democratic, patriotism, national spirit, communicative, peace loving, care for<br />the environment and responsibility based on Pancasila and the 1945 Constitution. The formation of leadership character is done by integrating the values of Sufism and nationalism in teaching and learning activities, pesantren culture and self-development so that the following characters emerge: theocentric, voluntary in serving, wisdom (fa</span><span class="fontstyle2">ṭ</span><span class="fontstyle0">anah), simplicity, independence, altruism, sincerity, nationalism, responsibility (amanah), role<br />modeling, moderate, visionary. The synergy of the values of Sufism and nationalism was applied through the concepts and processes of education that were organized by the pesantren. The synergy produced the values of Sufism which was nuanced by nationalism and created a new educational model namely Sufism-Nationalism Education .</span>