Soxhlet Extraction of Cannabinoids (CBD and THC) From Four Different Strains of Cannabis Grown in Four Different Regions

The objective of this work was to investigate the cannabinoid content of extracts which was conducted by soxhlet extraction. In the extraction system hexane, ethyl acetate and ethanol were used sequentially to extract cannabis plant. When all species were evaluated in terms of CBD, all results in Meram region were the highest. As it can be understood, if an efficient extract in terms of CBD is desired, sowing can be done in Meram. Narlı strain provided the highest (65.88%) yield in terms of CBD in accordance with its cultivation purpose. When evaluated THC active ingredient, it was seen that each strain gave high yields in different regions. Elnur strain yielded the highest yields in Altınekin (66.21%), Papatya in Beyşehir (52.05%), Narlı (14.49%) and Gökçeağaç in Çumra (56.13%). It is the first study to show the different climatic conditions effect on the yield of CBD and THC in different strains.

DETERMINATION OF MOLECULAR WEIGHT AND BOILING TEMPERATURE OF UNKNOWN COMPONENTS OF A MIXTURE BY MEANS OF CHROMATE-DISTRIBUTIVE METHOD

New technique, allowing to increase the accuracy of determination of molecularweight andboiling temperature of unknown components of analyzing mixture, in comparison with our work described in the journal ”Sorption and Chromatographic Processes” 2011, № 4. and based on information on sizes of indexes of retention of Van den Dool and Krats and indexes of the logarithm of constant of distribution in the system ”hexane - acetonitrile”is suggested.

Selective regional alterations in the content or distribution of neuronal and glial cytoskeletal proteins in brain of rats chronically exposed to 2,5-hexanedione

Hexane is used in many industrial processes and induces neurotoxic effects in the central and peripheral nervous system. Hexane is metabolized to 2,5-hexanedione, which is the neurotoxic agent. Continued exposure to hexane or 2,5-hexanedione results in loss of sensorial and motor function in arms and legs and to alterations in axonal neurofilament proteins. The effects of 2,5-hexanedione on different cytoskeletal proteins in different brain areas have not been studied in detail. The aim of this work was to study the effects of chronic exposure of rats to 2,5-hexanedione (1% in the drinking water) on tubulin, neurofilament NF-L, microtubule-associated protein MAP-2, and on glial fibrillary acidic protein (GFAP), in cerebellum, hippocampus and cerebral cortex. The amount of each protein was determined by immunoblotting and its distribution was analysed by immunohistochemistry. The results obtained show a regional selectivity in the 2,5-hexanedione effects on cytoskeletal proteins. NF-L content decreased in all brain areas. MAP-2 decreased in cerebellum and hippocampus and tubulin decreased only in cerebellum. GFAP decreased only in cerebral cortex, but its distribution was altered in cerebellum, with increased content in the granular layer and decreased content in the molecular layer. The area most affected was the cerebellum, where all the proteins analysed were altered. These cytoskeletal proteins alterations may impair the transfer of information involved in the regulation by the cerebellum of motor function and contribute to the altered motor performance in rats exposed to 2,5-hexanedione and humans exposed to hexane.