Reversal of Experimental Liver Damage after Transplantation of Stem-Derived Cells Detected by FTIR Spectroscopy

The transplantation of autologous BM-MSCs holds great potential for treating end-stage liver diseases. The aim of this study was to compare the efficiency of transplanted rBM-MSCs and rBM-MSC-derived differentiated stem cells (rBM-MSC-DSCs) for suppression of dimethylnitrosamine-injured liver damage in rat model. Synchrotron radiation Fourier-transform infrared (SR-FTIR) microspectroscopy was applied to investigate changes in the macromolecular composition. Transplantation of rBM-MSC-DSCs into liver-injured rats restored their serum albumin level and significantly suppressed transaminase activity as well as the morphological manifestations of liver disease. The regenerative effects of rBM-MSC-DSCs were corroborated unequivocally by the phenotypic difference analysis between liver tissues revealed by infrared spectroscopy. Spectroscopic changes in the spectral region from 1190–970 cm−1 (bands with absorbance maxima at 1150 cm−1, 1081 cm−1, and 1026 cm−1) indicated decreased levels of carbohydrates, in rBM-MSC-DSC-transplanted livers, compared with untreated and rBM-MSC--transplanted animals. Principal component analysis (PCA) of spectra acquired from liver tissue could readily discriminate rBM-MSC-DSC-transplanted animals from the untreated and rBM-MSC-transplanted animals. We conclude that the transplantation of rBM-MSC-DSCs effectively treats liver disease in rats and SR-FTIR microspectroscopy provides important insights into the fundamental biochemical alterations induced by the stem-derived cell transplantation, including an objective “signature” of the regenerative effects of stem cell therapy upon liver injury.

HEPATOPROTECTIVE ACTION OF POLAR LIPIDS OF MARAL ANTLERS AND PEAT IN EXPERIMENTAL LIVER DAMAGE CAUSED BY ISONIAZIDE AND PARACETAMOL

In experimental liver pathology caused by isoniazide or paracetamol administration to albino rats lipids derived from maral antlers and peat decreased the blood activity of aminotransferases, γ-glutamyltranspeptidase, acid and alkaline phosphatases, phospholipase А, content of common bilirubin, activated the detoxication of biliribin, ammonium and phenols, inhibited the liver formation of dienic conjugates, Schiff’s bases, malonic dialdehyde, improve the reduced glutathione function. Maral anthlers and peat lipids in effective doses 30 and 60 mg/kg had the more pronounced hepatoprotective and antioxidant action than lipids in dose 10 mg/kg and essentiale forte N.

Influence of the Escherichia coli Nissle 1917 strain on complications of the chronic experimental liver damage

The aim of the study was evaluate the influence of the probiotic <i>Escherichia coli</i> Nissle 1917 strain (Mutaflor&reg; suspension, Ardeypharm GmbH, Herdecke, Germany) on bacterial translocation in cases of liver damage, damage to the intestinal mucosa, potential portal hypertension associated with possible development of oesophageal varices and on the bacterial population of the intestine during chronic experimental liver damage in the laboratory rat. Rats with liver damage induced by thioacetamide were divided into an experimental and control group. Experimental and control animals were applied Mutaflor and saline, respectively. Samples of blood, liver, lymph nodes and caecum for microbiological examination, of liver, duodenum and oesophagus for histological examination and of spleen for weight evaluation were collected. There were no significant differences between both groups of animals in the qualitative proportion of <i>Staphylococcus</i> spp., <i>Enterococcus</i> spp. and <i>Proteus</i> spp. cultured from the lymph nodes, blood and liver. The quantitative culture results on <i>Enterococcus</i> spp. in the caecum, liver and lymph nodes showed no significant differences between both groups. There was a significant difference between the experimental and control group in the counts of coliform bacteria. No significant differences between both groups were found in the overall damage score of the liver, duodenum and oesophagus. There were no differences in the spleen to body weight ratio of both groups. The application of Mutaflor&reg; suspension for eight days had no recognisable effect diminishing the selected complications of chronic liver damage caused by the administration of TAA to laboratory rats.