Automatic system for optical parameters measurements of biological tissues

In this paper a system allowing execution of automatic measurements of the optical parameters of scattering materials in a efficient and accurate manner is proposed and described. The system is designed especially for measurements of biological tissues including phantoms, which closely imitate optical characteristics of a real tissue. The system has modular construction and is based on ISEL system, luminance and color meter and a computer with worked out dedicated software and user interface. Performed measurements of scattering distribution characteristics for selected materials revealed good accuracy, confirmed by comparative measurements using well-known reference characteristics. Full Text: PDF ReferencesWróbel, M. S., Popov, A. P., Bykov, A. V., Kinnunen, M., Jedrzejewska-Szczerska, M., & Tuchin, V. V. (2015). Measurements of fundamental properties of homogeneous tissue phantoms. Journal of Biomedical Optics CrossRef Wróbel, M. S., Jedrzejewska-Szczerska, M., Galla, S., Piechowski, L., Sawczak, M., Popov, A. P., Cenian, A. (2015). Use of optical skin phantoms for preclinical evaluation of laser efficiency for skin lesion therapy. Journal of Biomedical Optics. CrossRef Jędrzejewska-Szczerska, M., Wróbel, M. S., Galla, S., Popov, A. P., Bykov, A. V., Tuchin, V. V., & Cenian, A. (2015). Investigation of photothermolysis therapy of human skin diseases using optical phantoms. In Proceedings of SPIE - The International Society for Optical Engineering. CrossRef Brown A. M., et al.: Optical material characterization through BSDF measurement and analysis, Proc. of SPIE, Vol. 7792, 2010 CrossRef 4-Axis Controller: iMC-S8. Operating Instruction. ISEL Germany AG, 2012. DirectLink Konica Minolta, Inc. (2005-2013). Chroma meter CS-200. Datasheet. DirectLink Malacara D.: Color Vision and Colorimetry; Theory and Applications, SPIE Press, 2002. DirectLink A. Mazikowski, M. Trojanowski: Measurements of Spectral Spatial Distribution of Scattering Materials for Rear Projection Screens used in Virtual Reality Systems, Metrology and Measurement Systems, 20 (3), pp. 443 - 452, 2013 CrossRef

Anatomie Et Histologie De L’hepatopancreas Chez Le Male Du Poisson Brachydeuterus Auritus (Haemulidae, Valenciennes, 1831) En Cote D’ivoire

Brachydeuterus auritus is a fish species with a high level of exploitation potential in Côte d'ivoire. The following study is based upon a maturity ladder and the discrimination of the hepatopancreas. The methodological approach consisted of a macroscopic description of the hepatopancreas according to the five stages of sexual maturity. The microscopic study consisted of fixing the hepatopancreas, dehydrating, impregnating, including it, making cuts that are colored for observation. At the macroscopic level, the hepatopancreas is made of two unequal lobes. The organ has a colouring as well as a vascularization varying in terms of the species development stages. At the microscopic level among the males, the hepatopancreas is a mixed organ made of the liver and the pancreas. The hepatopancreatic parenchyma is an homogeneous tissue made of hematocytes which are polygonal cells. In addition, we can find hematopoietic tissues in the parenchyma and melanomacrophages centers located near the blood vessels and the biliary tracts. The melanomacrophages centers are formed with cells accumulating pigment cells. Inside the organ, the pancreas is discriminated centripally and constituted of exocrine cells and Langerhans islets. The physiology of the two entities (liver and pancreas) will establish the organ's physiology. Hepatopancreas is involved in reproduction, digestion and defence. It exists as an organ that controls many functions and plays an important part in the physiology of fish in anabolism and catabolism.

Electrostatic Capture Following Laser Microdissection for the Preparation of Homogeneous Biological Specimens

There is an unmet need for researchers in life sciences and clinical pathology to obtain untainted target cells with very high accuracy, which are suitable for subsequent genome and protein analysis. In this paper, an electrostatic capture laser microdissection technology (ECM) is proposed and explained. Following microscopic identification and separation of target cells from the surrounding tissues using laser cutting, the ECM uses electrostatic forces to capture target cells. Validation experiments indicate that ECM can capture a wide assortment of contamination-free homogeneous samples, ranging from very tiny pieces of a few micrometers in diameter to large pieces with a surface area of over 40,000 µm2. Evidence is also provided indicating that uncontaminated homogeneous tissue materials collected by ECM are suitable for further DNA and RNA analysis. This suggests that ECM capture causes little or no identifiable damage to the collected tissues. This technique has significant advantages compared with existing traditional capture methods, such as very low risk of biological sample damage and the fact that it can be applied to both upright and inverted microscopy. The latter allows for separating target cells in tissue culture. ECM usage provides a cost-effective alternative to more traditional laser capture microdissection techniques.