Apoprotein E and Reverse Cholesterol Transport

Apoprotein E (apoE) is a multifunctional protein. Its best-characterized function is as a ligand for low-density lipoprotein (LDL) receptor family members to mediate the clearance of apoB-containing atherogenic lipoproteins. Among its other functions, apoE is involved in cholesterol efflux, especially from cholesterol-loaded macrophage foam cells and other atherosclerosis-relevant cells, and in reverse cholesterol transport. Reverse cholesterol transport is a mechanism by which excess cellular cholesterol is transported via lipoproteins in the plasma to the liver where it can be excreted from the body in the feces. This process is thought to have a role in the attenuation of atherosclerosis. This review summarizes studies on the role of apoE in cellular cholesterol efflux and reverse cholesterol transport and discusses the identification of apoE mimetic peptides that may promote these pathways.

Effects of proteinuria on apolipoprotein metabolism disorders

Introduction Proteinuria causes lipid metabolism abnormalities. Material and methods The study included 60 patients (M:F 32:28), mean age 37.15? 9.85 years, with average glomerular filtration rate (GFR) 86.27? 19.81 mL/min, and average BMI 24.18?2.23 kg/m2. Regarding the level of glomerular proteinuria, patients were divided into four groups. The first group, with proteinuria levels less than 0.25 g/24h, included 15 patients (M:F 6:9) (control group), average age 34.66?4.82 years, with mean GFR 99.70? 12.94 mL/min, and mean BMI 23.28?3.50 kg/nr. The second group, with proteinuria between 0.26 and 1.0 g/24h, comprised 15 patients (M:F 9:6) with primary glomerulonephritis, mean age 37.87?9.65 years, with mean GFR 82.85?18.48 mL/min and mean BMI 23.83?1.57 kg/m2. The third group consisted of 15 patients (M:F 8:7) with primary glomerulonephritis, with proteinuria between 1.1 and 3.0 g/24h, average age 35.67? 13.29 years, mean GFR 82.85?I8.48 mL/min, and mean BMI 23.83?1.57 kg/m2. The fourth group, with proteinuria over 3.0 g/24h, included 15 patients (M:F 9:6) with primary' glomerulonephritis, mean age 40.40?9.75 years, with mean GFR 80.16?20.80 mL/min, and mean BMI 24.83?1.44 kg/m2. In order to assess the influence of proteinuria on apoprotein metabolism abnormalities we investigated apo A-l apo B, apo E and the apo A-I/apo B ratio. Results Results were statistically analyzed using Student's t-test, and the Mann-Whitney U test. Statistically, patients with proteinuria over 3.0 g/24h have significantly higher apoprotein B and apoprotein E values in the serum, as well as apo B/apo A-l compared to control subjects, and patients with proteinuria around 0.26-1.0 g/24h and proteinuria around 1.1-3.0 g/24h. Proteinuria leads to deterioration of apoprotein abnormalities. .