chlamydomonas mutants
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2016 ◽  
Vol 92 (6) ◽  
pp. 629-641 ◽  
Author(s):  
Sari Dewi Kurniasih ◽  
Tomohito Yamasaki ◽  
Fantao Kong ◽  
Sigeru Okada ◽  
Dwiyantari Widyaningrum ◽  
...  

2015 ◽  
Vol 26 (15) ◽  
pp. 2810-2822 ◽  
Author(s):  
Tomohiro Kubo ◽  
Masafumi Hirono ◽  
Takumi Aikawa ◽  
Ritsu Kamiya ◽  
George B. Witman

Ciliary length control is an incompletely understood process essential for normal ciliary function. The flagella of Chlamydomonas mutants lacking multiple axonemal dyneins are shorter than normal; previously it was shown that this shortness can be suppressed by the mutation suppressor of shortness 1 ( ssh1) via an unknown mechanism. To elucidate this mechanism, we carried out genetic analysis of ssh1 and found that it is a new allele of TPG2 (hereafter tpg2-3), which encodes FAP234 functioning in tubulin polyglutamylation in the axoneme. Similar to the polyglutamylation-deficient mutants tpg1 and tpg2-1, tpg2-3 axonemal tubulin has a greatly reduced level of long polyglutamate side chains. We found that tpg1 and tpg2-1 mutations also promote flagellar elongation in short-flagella mutants, consistent with a polyglutamylation-dependent mechanism of suppression. Double mutants of tpg1 or tpg2-1 and fla10-1, a temperature-sensitive mutant of intraflagellar transport, underwent slower flagellar shortening than fla10-1 at restrictive temperatures, indicating that the rate of tubulin disassembly is decreased in the polyglutamylation-deficient flagella. Moreover, α-tubulin incorporation into the flagellar tips in temporary dikaryons was retarded in polyglutamylation-deficient flagella. These results show that polyglutamylation deficiency stabilizes axonemal microtubules, decelerating axonemal disassembly at the flagellar tip and shifting the axonemal assembly/disassembly balance toward assembly.


PLoS ONE ◽  
2013 ◽  
Vol 8 (4) ◽  
pp. e61851 ◽  
Author(s):  
Maya D. Lambreva ◽  
Maria Teresa Giardi ◽  
Irene Rambaldi ◽  
Amina Antonacci ◽  
Sandro Pastorelli ◽  
...  

Cytoskeleton ◽  
2009 ◽  
Vol 67 (2) ◽  
pp. 71-80 ◽  
Author(s):  
Mei Wei ◽  
Priyanka Sivadas ◽  
Heather A. Owen ◽  
David R. Mitchell ◽  
Pinfen Yang

2008 ◽  
pp. 1093-1097 ◽  
Author(s):  
Xenie Johnson ◽  
Richard Kuras ◽  
Francis-André Wollman ◽  
Olivier Vallon

2005 ◽  
Vol 46 (3) ◽  
pp. 489-496 ◽  
Author(s):  
Isao Kobayashi ◽  
Shoko Fujiwara ◽  
Kosuke Shimogawara ◽  
Chiseko Sakuma ◽  
Yasuo Shida ◽  
...  

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