Intrinsic basis of thermostability of prolyl oligopeptidase from Pyrococcus furiosus

Salt-bridges play a key role in the thermostability of proteins adapted in stress environments whose intrinsic basis remains to be understood. We find that the higher hydrophilicity of PfP than that of HuP is due to the charged but not the polar residues. The primary role of these residues is to enhance the salt-bridges and their ME. Unlike HuP, PfP has made many changes in its intrinsic property to strengthen the salt-bridge. First, the desolvation energy is reduced by directing the salt-bridge towards the surface. Second, it has made bridge-energy more favorable by recruiting energetically advantageous partners with high helix-propensity among the six possible salt-bridge pairs. Third, ME-residues that perform intricate interactions have increased their energy contribution by making major changes in their binary properties. The use of salt-bridge partners as ME-residues, and ME-residues' overlapping usage, predominant in helices, and energetically favorable substitution are some of the favorable features of PfP compared to HuP. These changes in PfP reduce the unfavorable, increase the favorable ME-energy. Thus, the per salt-bridge stability of PfP is greater than that of HuP. Further, unfavorable target ME-residues can be identified whose mutation can increase the stability of salt-bridge. The study applies to other similar systems.

FORMATION OF HUMAN HEALTHY LIFESTYLE OF THE TRAINED YOUTH ORELSUET

Health is the greatest value of a person. Modern trends in all spheres of Russian society have stipulated the change the intrinsic basis of educational organization. It is values aimed at maintenance of health of the rising generation, creation of conditions in the university for for-mation of health culture of the students and accustom them to healthy lifestyle. For today the theme is rather actual. Therefore in article we will consider the core criteria of formation healthy lifestyle of the students.

Motif of struggle in the chronicles "The Cathedral Clergy" by Nikolai Leskov

The author of the article addresses history of the publication of the chronicles "The Cathedral Clergy" by Nikolai Leskov; she notes that the subject and the core conflict of the work collided with its publication in a number of magazines, and that only Mikhail Katkov realised the chronicle's importance and accepted it in his magazine "The Russian Messenger". In "The Cathedral Clergy", Nikolai Leskov looks at the world from a special perspective which opens his point of view as an eternity look, whereas what becomes the core conflict in the work, is confrontation of belief and unbelief of global, if not universal, scale. Realisation of one of the book's most significant motifs – motif of struggle – is analysed in the article. It is considered how Nikolai Leskov on a set of examples illustrates the heroes' active and energetic strength that is shown in advocacy of belief, in resistance to meanness and premeditated deception. The writer very thinly and skillfully shows that fight is not an intrinsic basis of righteous people, that all of them live under the law of love, however they cannot be passive observers in the world where the truth is profaned.