Synthesis of Platinum Nanoparticles from K2PtCl4 Solution Using Bacterial Cellulose Matrix

Platinum (Pt) nanoparticles have been synthesized from a precursor solution of potassium tetrachloroplatinate (K2PtCl4) using a matrix of bacterial cellulose (BC). The formation of Pt nanoparticles occurs at the surface and the inside of the BC membrane by reducing the precursor solution with a hydrogen gas reductant. The Pt nanoparticles obtained from the variations of precursor concentration, between 3 mM and 30 mM, and the formation of Pt nanoparticles have been studied using X-ray diffraction (XRD), scanning electron microscopy-energy dispersive X-ray spectroscopy (SEM-EDS), and thermogravimetry analysis (TGA). Based on X-ray diffraction patterns, Pt particles have sizes between 6.3 nm and 9.3 nm, and the Pt particle size increases with an increase in precursor concentration. The morphology of the Pt nanoparticles was observed by SEM-EDS and the content of Pt particles inside the membrane is higher than that on the surface of BC membranes. This analysis corresponds to the TGA analysis, but the TGA analysis is more representative in how it describes the content of Pt particles in the BC membrane.

Synthesis of di-pyropheophorbide-a-platinum(II) complex and the in vitro cytotoxicity against TC-1 tumor cells

This report focuses on the conjugation of a chemotherapeutic drug and photodynamic therapy (PDT) agent with the intention of obtaining an optimal anticancer agent for use in combination cancer treatment. We have used pyropheophorbide-a (PPa) as a PDT agent, which was linked with ethylenediamine using carbodimide/N-hydroxysuccinicimide coupling reagents to obtain a resulting reactive amine group. Next, the free amine-bearing PPa and potassium tetrachloroplatinate (K2PtCl4) were reacted in aqueous solution to obtain PPa-cisplatin complex. Subsequently, the PPa-based platinum complex was characterized through several methods including NMR spectroscopy, mass spectrometer and UV absorption spectroscopy and its in vitro cytotoxicity and cellular uptake were evaluated on the TC-1 cancer cell line. The results indicated that the conjugate has a greater cytotoxic effect in the dark than cisplatin, even though its phototoxic effect was slightly lower than that of free photosensitizer; in addition, the cellular uptake of complex at 0.125 and 0.25 μM was higher than that of free photosensitizer.

Synthesis, Characterization and Antitumor Activities of Cis-gallic acid[(1R,2R)-1,2-cyclohexanediamine] Platinum(II)

In order to obtain a gallic acid platinum complex, the title compound was synthesed from potassium tetrachloroplatinate by the reaction potassium iodide, (1R,2R)-1,2-cyclohexanediamine, silver nitrate and gallic acid respectively. The complex structure and component were characterized by elemental analysis, ESI-MS, 1H-NMR and IR and the anticancer activity was measured by MTT method. The result showed that its structure was consistent with the title compound and had inhibiting effect on the growth of tumor cell lines in vitro.