Alabio ducks, germplasms of South Kalimantan, as protein and income sources

Apart from providing food originating from meat and eggs, Alabio ducks have another important role for farmers in South Kalimantan, namely as an income source, employment source, and preservation of local genetic source. This paper aimed to study the economic profile of Alabio ducks in several types of businesses in Hulu Sungai Utara Regency, South Kalimantan. A survey method was carried out on several groups of duck farmers. In order to obtain more in-depth information, a focus group discussion (FGD) was held with key insiders in the duck industry. The results showed that the duck farming business has good prospects and can be a source of income and employment for farmers and young generations. The business of hatchery, slaughter-duck producers, hatching egg producers, and consumes-egg producers, is economically feasible. Improving bio-security and handling and managing environmental sanitation have to carry out, among others, through pens, equipment, and environmental disinfection, as well as routine vaccinations with the escort and support of local and central government. In addition, to increase the welfare and income of farmers need to introduce technological innovations that can increase farming efficiency.

Successful Treatment of Nannizziopsis guarroi Infection using Systemic Terbinafine in a Central Bearded Dragon (Pogona vitticeps)

Nannizziopsis guarroi infection in lizards presents therapeutic challenges as reports of poor clinical outcomes, including antifungal toxicity, incomplete clearance of infection and recrudescence of infection are common. The case presented here describes the successful treatment of a N. guarroi infection using systemic terbinafine and environmental disinfection in a captive-bred central bearded dragon (Pogona vitticeps). The lizard presented with darkly colored cutaneous lesions and mycologic culture samples were identified as N. guarroi using Matrix-Assisted Laser Desorption/Ionization – Time Of Flight (MALDI-TOF). Based upon the lack of clinical resolution of cutaneous lesions, weight loss, and reduced appetite, initial treatment with voriconazole was discontinued. Terbinafine was prescribed and weekly environmental disinfection with sodium hypochlorite was initiated until cutaneous clearance of the fungus was confirmed by negative culture, histopathology, and N. guarroi qPCR from cutaneous swab. Terbinafine treatment was discontinued after 80 days. There were no clinical signs of toxicity associated with the prolonged treatment and the lizard has not developed any cutaneous lesions or illness in more than two years of clinical follow up. While the most ideal treatment of N. guarroi is still being investigated, this case demonstrates a promising and safe treatment option for an increasingly common and devastating disease.

The impact of COVID-19 on knowledge, attitude, and infection control behaviors among dentists

Background This study seeks to elucidate the impact of COVID-19 on knowledge, attitude, and infection control behaviors among dentists. Methods Changes in knowledge, attitude, and infection control behaviors reported in 2020 (COVID-19 period) were compared to the historical control of the non-COVID-19 period in 2018. A proportional random sampling method was used to select the study samples from 400 dental institutions. The response rate was 69% in 2018 and 62.8% in 2020. A total of 276 dentists in 2018 and 251 dentists in 2020 responded to this questionnaire. Multiple logistic regression was used to assess the associations between factors and recommended infection control practices. Results High rates of correct COVID-19 knowledge (94.76%), fears of being infected with the virus (94%) and use of personal protective equipment (mask, glove and protection gown; 95%) were reported. We found that knowledge regarding environmental infection control, HIV transmission, and the window of HIV transmission were significantly higher in the post-COVID-19 period compared with the pre-COVID-19 period. High compliance rates of wearing mask, gloves and protection were reported. The number of dentists wearing a hair cap and a protective eye mask/face shield during the pandemic significantly increased compared with that noted before the COVID-19 pandemic. Factors associated with the use of a hair cap and an eye mask/face shield differed between the pre- and post-COVID-19 periods. The factors associated with compliance regarding environment infection control also differed between the pre- and post-COVID-19 periods. Conclusion The significant impact of COVID-19 on the knowledge, attitude, and infection control behaviors among dental care workers was observed in the current study. In particular, the use of hair caps and protective eye mask or face shields as well as environmental disinfection protocols has significantly improved. Trial registration TMU-JIRB: N201804006.

Ranking the Effectiveness of Non-Pharmaceutical Interventions to Counter COVID-19 in UK Universities with Vaccinated Population

Several universities around the world have resumed in-person teaching after successful vaccination campaigns have covered 70/80% of the population. In this study, we combine a new compartmental model with an optimal control formulation to discover, among different non-pharmaceutical interventions, the best prevention strategy to maximize on-campus activities while keeping spread under control. Composed of two interconnected Susceptible-Exposed-Infected-Quarantined-Recovered (SEIQR) structures, the model enables staff-to-staff infections, student-to-staff cross infections, student-to-student infections, and environment-to-individual infections. Then, we model input variables representing the implementation of different non-pharmaceutical interventions and formulate and solve optimal control problems for four desired scenarios: minimum number of cases, minimum intervention, minimum non-quarantine intervention, and minimum quarantine intervention. Our results reveal the particular significance of mask wearing and social distancing in universities with vaccinated population (with proportions according to UK data). The study also reveals that quarantining infected students has a higher importance than quarantining staff. In contrast, other measures such as environmental disinfection seems to be less important.

90. Impact of Infection Control Assessment and Response (ICAR) Visit on Candida auris Colonization Rates at Seven Long Term Acute Care Hospitals (LTACH) in Los Angeles County

Background Public health authorities often use Infection Control Assessment and Response (ICAR) visits during Candida auris (C. auris) outbreak investigation to identify facility-level infection prevention and control (IPC) practice gaps and make recommendations to address those gaps. As an adjunct to ICAR visit, point prevalence surveys (PPS) provide an objective measure to determine if IPC recommendations are implemented. Because they require significant public health resources to perform, we evaluated the impact of ICAR visits on C. auris colonization rates. Methods PPS were conducted at seven long-term acute-care hospitals (LTACH) with C. auris outbreaks in Los Angeles County from July 2020 to May 2021. Skin swabs collected at PPS were tested for C. auris colonization by PCR technique. Pre-ICAR PPS results were compared with the average of two serial post-ICAR PPS results using repeated measures ANOVA test. Linear regression was used to estimate associations between individual ICAR domains and C. auris colonization. Results 54 PPS were conducted at seven LTACHs with at least one ICAR visit made for every two PPS. On average, PPS were conducted 14 days (range 1-15 days) before and 10 days (range 4-33 days) after an ICAR visit. PPS positive rates with ICAR visit dates for each LTACH are shown in figure 1. Overall, ICAR visits were associated with a significant decrease (p=0.035) in the average of the positive rates in two serial post-ICAR PPS. When individual domain (hand hygiene, contact precautions, and environmental disinfection) of ICAR tool was analyzed, only adherence to environmental disinfection was significantly associated (p=0.038) with decrease in C. auris colonization rates. There was a moderate negative correlation (R2 = 0.26, β= -0.33) between environmental disinfection adherence and the magnitude of decrease in the colonization rates across all LTACHs (Figure 2). Figure 1 Figure 2 Conclusion ICAR visits were found to be significantly associated with a decrease in the average PPS positive rate on serial PPS. Parts of the ICAR tool that assessed environmental disinfection at the facility seemed most correlated with decrease in C. auris colonization rate. Streamlining the ICAR process to focus on the most impactful parts of ICAR tool may be a more efficient intervention to control C. auris outbreaks. Disclosures All Authors: No reported disclosures

Dufan (Dunia Fantasi) Ancol di Era Pandemi Covid-19

The enactment of government policies to stop the spread of the virus, requires DUFAN to face new habitual adaptation situations by implementing health protocols, devising various strategies to ensure visitors even if limited movement can still have fun safely. This research discusses the adaptation efforts of tourist objects in the face of the COVID-19 pandemic. It is hoped that this research can become input in planning the adaptation of tourist destination. The qualitative research method used purposive sampling, the collection technique used triangulation (combined) and inductive data analysis. The results of the research are operational observation data of DUFAN, the obstacles faced and the perception of visitors in the pandemic era. DUFAN's operational activities conform to the Taman Impian Jaya Ancol policy. DUFAN's operational reopening was accompanied by various regulations; restrictions on visitors, online ticket reservations, check ticket and body temperature. Availability of hand sanitizer facilities and standing banner for health protocol regulations. There is a surveillance section that always goes around the entire DUFAN area and an environmental disinfection section. The implementation of the health protocol in the operation of game rides is carried out well, however, there are several obstacles faced. The main obstacle is the awareness of visitors about the importance of adhering to health protocols anytime anywhere in the DUFAN area smoking carelessly, not wearing a mask during activities in the DUFAN environment, violation of distance restrictions in queues. The perception of DUFAN visitors in the era of the COVID-19 pandemic was quite good as assessed in terms of operational management systems, online ticket purchases, health protocol information, health protocols for visitors and tourist officers, availability of hand washing facilities, and safety of traveling in DUFAN. Visitors expect more tourist officers at DUFAN to patrol to remind visitors to apply health protocols, especially physical distancing.

Antimicrobial Effects of Novel H2O2-Ag+ Complex on Membrane Damage to Staphylococcus aureus,Escherichia coli and Salmonella typhimurium

Diseases caused by harmful microorganisms pose a serious threat to human health. Safe and environment-friendly disinfectants are, therefore, essential in preventing and controlling such pathogens. This study aimed to investigate the antimicrobial activity and mechanism of a novel hydrogen peroxide and silver (H 2 O 2 -Ag + ) complex (HSC) in combatting Staphylococcus aureus ATCC 29213, Escherichia coli O157:H7 NCTC 12900 and Salmonella typhimurium SL 1344. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values against S. aureus were found to be 0.014 % H 2 O 2 -3.125 mg/L Ag + , while 0.028 % H 2 O 2 -6.25 mg/L Ag + for both E. coli and S. typhimurium . Results of the growth curve assay and time-kill trial suggest that the HSC could inhibit the growth of the tested bacteria, as 99.9 % of viable cells were killed following treatment at the 1 MIC for 3 h. Compared with Oxytech D10 disinfectant (0.25 % H 2 O 2 -5 mg/L Ag + ), the HSC exhibited better antibacterial efficacy at a lower concentration (0.045 % H 2 O 2 -10 mg/L Ag + ). The mechanism of antibacterial action of HSC was found including the disruption of the bacterial cell membrane, followed by entry into the bacteria cell to reduce intracellular adenosine triphosphate (ATP) concentration, and inhibit the activity of antioxidases, superoxide dismutase (SOD) and catalase (CAT). The enhanced bactericidal effect of hydrogen peroxide combined with silver indicates a potential for its application in environmental disinfection, particularly in the food industry.

SARS-CoV-2 Disinfection of Air and Surface Contamination by TiO2 Photocatalyst-Mediated Damage to Viral Morphology, RNA, and Protein

SARS-CoV-2 is the causative agent of COVID-19, which is a global pandemic. SARS-CoV-2 is transmitted rapidly via contaminated surfaces and aerosols, emphasizing the importance of environmental disinfection to block the spread of virus. Ultraviolet C radiation and chemical compounds are effective for SARS-CoV-2 disinfection, but can only be applied in the absence of humans due to their toxicities. Therefore, development of disinfectants that can be applied in working spaces without evacuating people is needed. Here we showed that TiO2-mediated photocatalytic reaction inactivates SARS-CoV-2 in a time-dependent manner and decreases its infectivity by 99.9% after 20 min and 120 min of treatment in aerosol and liquid, respectively. The mechanistic effects of TiO2 photocatalyst on SARS-CoV-2 virion included decreased total observed virion count, increased virion size, and reduced particle surface spike structure, as determined by transmission electron microscopy. Damage to viral proteins and genome was further confirmed by western blotting and RT-qPCR, respectively. The multi-antiviral effects of TiO2-mediated photocatalytic reaction implies universal disinfection potential for different infectious agents. Notably, TiO2 has no adverse effects on human health, and therefore, TiO2-induced photocatalytic reaction is suitable for disinfection of SARS-CoV-2 and other emerging infectious disease-causing agents in human habitation.

Pencegahan Stigma Terhadap Orang dalam Penyebutan Covid-19

There are still many people who do not know and understand the COVID-19 virus, which is still new. Some of the news that can be accessed on news search links show a lot of oppression and stigma against people called COVID-19. Stigma, among other things, arises because society ignores new diseases and there is no cure, stigma can be suppressed and lost if society finds out about it. Generally, service activities are conducted to provide knowledge and understanding of COVID-19 and how to prevent it. Stigma prevention is done by environmental disinfection activities, mask distribution for adults and children as well as counseling on clean and healthy living habits and physical distance, disinfection activities and mask distribution done to give a sense of peace to the population. Clean and healthy lifestyle guidance activities and physical distance are done to provide correct information. Generally, service activities are conducted to provide knowledge and understanding of COVID-19 and how to prevent it. With the right knowledge, stigma will not occur in society. This service can improve people's knowledge and understanding of COVID-19, live a new life pattern, and understand the attitudes and views that should be present when dealing with people called COVID-19 so that people can live their daily lives calmly and vigilantly