T-cadherin in epithelial ovarian cancer: relationship with cancer progression and drug resistanceT-cadherin in epithelial ovarian cancer: relationship with cancer progression and drug resistance

Background: T-cadherin plays a crucial role in maintaining normal tissue structure by regulating specific cell adhesion, cellular recognition and signal transduction. The purpose of this study was to evaluate whether T-cadherin influences epithelial ovarian cancer (EOC) progression, differentiation and drug resistance and its possible mechanisms.Methods: Epithelial ovarian carcinoma (EOC, n=63) and relevant contralateral normal ovarian (CNO, n=41) fresh tissues were collected from epithelial ovarian carcinoma patients, and benign ovarian tumour fresh tissues were collected from 55 patients with benign ovarian tumours. The human epithelial ovarian carcinoma cell line A2780 was cultured. T-cadherin expression was assessed by real-time RT-PCR and Western blotting, and the expression of matrix metalloproteinase-2 (MMP-2) was detected by Western blotting. pcDNA‑T‑cad plasmid technology was used to upregulate T-cadherin expression. In addition, A2780 cell migration and invasion ability, viability, colony formation, proliferation, apoptosis and sensitivity to paclitaxel were measured.Results: T‑cadherin mRNA and protein expression in EOC tissues from EOC patients was significantly downregulated, and there was no significant difference between the matched contralateral normal ovarian fresh tissue from the same patient and the benign ovarian tumour tissues from other patients. The migration and invasion abilities, viability, colony formation, and proliferation were attenuated by restoration of T‑cadherin expression in A2780 cells via pcDNA‑T‑cad transfection; apoptosis, MMP-2 expression and sensitivity to Taxol were also enhanced by restored T‑cadherin expression. The T‑cadherin expression level was well correlated with the clinical characteristics and symptoms of EOC patients, including tumour stage, histology, lymph node metastasis, tumour size, distant metastasis and cisplatin resistance.Conclusions: T‑cadherin participates in the processes of epithelial ovarian carcinoma cell migration, invasion, proliferation, apoptosis and sensitivity to paclitaxel and can regulate the expression of MMP-2. Downregulation of T‑cadherin expression may contribute to epithelial ovarian cancer progression, differentiation and drug resistance.

D-DIMER AND FIBRINOGEN IN PATIENTS UNDERWENT SURGERY IN MALIGNANT AND BENIGN OVARIAN TUMOR (D-Dimer Dan Fibrinogen Pada Pasien-Pasien Yang Menjalani Tindakan Pembedahan Pada Tumor Ovarium Ganas Dan Jinak)

Ovarian tumour ranks second in gynecology tumour cases and ranks second in gynecology tumour death in Indonesia. Tumour cause hypercoagulable that increase risk of thrombosis by procoagulant mechanism. Tumout cells also can cause hyperfibrinogenemmia that can cause bleeding. The aim of study was to know D-dimer and fibrinogen value to investigate primary hyperfibrinolysis on malignant and benign ovarian tumour; and to know whether operation procedure on malignant and benign tumour change D-dimer and fibrinogen value. Prospective analysis study, subject were malignant and benign ovarian tumour patients undergoing surgery in Haji Adam Malik Hospital, Medan. One way Annova test dan Wilcoxon Sum-Rank test were performed. Stastical differentiation is indicate with p<0,05. Study subject was 16 patient where 8 malignant and benign ovarian tumour patient respectively. Malignant ovarian tumour D-dimer values was higher than benign ovarian tumour (p<0,01) that indicate fibrinolysis increase in malignant ovarian tumour. Malignant ovarian tumour fibrinogen values as same as benign ovarian tumour (p>0,05) that indicate the fibrinolysis in ovarian tumour wasn’t primary hyperfibrinolysis. Surgery procedure didn’t influence D-dimer and fibrinogen values. Primary hyperfibrinolysis wasn’t occur in ovarian tumour.Keywords : Ovarian tumour, malignant, benign, D-dimer, fibrinogen.

Serous cystadenoma of ovary with xanthogranulomatous oophoritis: combination of benign ovarian tumour with chronic inflammatory non-neoplastic pseudo-tumour-a rarity

Xanthogranulomatous inflammation is a rare type of chronic inflammation. It destroys the tissue of the affected organ, mostly affecting the kidney and gall bladder. Xanthogranulomatous inflammation of female genital tract being a rare entity, only few numbered cases involving the ovary have been reported in literature. This chronic inflammation of fallopian tubes and ovaries, usually present with pelvic masses, and on imaging and macroscopic appearance, are mostly misdiagnosed as ovarian neoplasm or tuberculosis. We are presenting a case of 14-year-old girl, who presented with an abdominal lump and on clinical and radiological investigations was suspected as ovarian malignancy but histo-pathology reported it as serous cystadenoma with xanthogranulomaotus oophoritis. The rarity of this case lies in the fact that rare destructive inflammatory features was present in a benign neoplasm of the ovary (serous cystadenoma) and that too in a youngest age reported till now.