Comparative Investigation on the Concentrations of Dehydroepiandrosterone (DHEA) and Testosterone Levels in Infertile Males attending a Private University Teaching Hospital, in Rivers State, Nigeria

The study was carried out to determine the concentrations of Dehydroepiandrosterone hormone (DHEAS) and testosterone in infertile males and compared with fertile males attending Madonna University Teaching Hospital (MUTH) Elele. Thirty apparently infertile males and 30 apparently fertile male as control had their Dehydroepiandrosterone hormone (DHEAS) and testosterone determined using competitive immune enzymatic colorimetric method and Enzyme Immunoassay while the semen analysis was done using Microscopy method. There was significant increase (P<0.05) in DHEAS of 1.23+0.07 ug/ml obtained in infertile male compared with 3.78 +0.13 ug/ml in the control. There was significant difference in Semen count of 56.27 +2.82million/ml in fertile males compared with 7.73+ 0.10 million/ml while testosterone in infertile males of 2.53+0.09 was significantly lower than 7.52+0.31 in fertile males(P<0.05). The study showed that DHEAS is elevated in infertility hence should be considered an indicator of infertility.

Somatic Embryogenesis Initiation in Sugi (Japanese Cedar, Cryptomeria japonica D. Don): Responses from Male-Fertile, Male-Sterile, and Polycross-Pollinated-Derived Seed Explants

This study aimed to obtain information from several embryogenic cell (EC) genotypes analyzing the factors that affect somatic embryogenesis (SE) initiation in sugi (Cryptomeria japonica, Cupressaceae) to apply them in the improvement of protocols for efficient induction of embryogenic cell lines (ECLs). The results of several years of experiments including studies on the influence of initial explant, seed collection time, and explant genotype as the main factors affecting SE initiation from male-fertile, male-sterile, and polycross-pollinated-derived seeds are described. Initiation frequencies depending on the plant genotype varied from 1.35 to 57.06%. The best induction efficiency was achieved when seeds were collected on mid-July using the entire megagametophyte as initial explants. The extrusion of ECs started approximately after 2 weeks of culture, and the establishment of ECLs was observed mostly 4 weeks after extrusion on media with or without plant growth regulators (PGRs). Subsequently, induced ECLs were maintained and proliferated on media with PGRs by 2–3-week-interval subculture routines. Although, the initial explant, collection time, and culture condition played important roles in ECL induction, the genotype of the plant material of sugi was the most influential factor in SE initiation.

73 Use of embryo transfer technology to salvage the germplasm of elite dromedary camels (Camelus dromedarius) infected with brucellosis

The present study was conducted to evaluate the potential of embryo transfer technology to salvage invivo-produced embryos from elite dromedary camels infected with brucellosis without transmission of the pathogen to the recipients or offspring. Ten elite racing champions who tested positive for brucella comprised the experimental group, whereas 3 donors negative for brucella acted as the control. Each donor animal received a combination of 2500IU of equine chorionic gonadotrophin (eCG; Folligon; Intervet), given as a single intramuscular injection on Day 1 of the treatment protocol, and 400mg of pFSH (Folltropin; Bioniche), injected twice daily in declining doses of 2×80mg, 2×60mg, 2×40mg, and 2×20mg over 4 days, also beginning on Day 1. They were mated with a fertile bull on the 11th day after the start of treatment. The donors of the group 1 received a combination of tetracycline, and streptomycin on alternate days for 2 weeks before mating with a fertile male. An injection of 20µg of gonadotrophin-releasing hormone (Receptal, Intervet) was given to them immediately after mating to induce ovulation. The embryos were collected by the non-surgical method on Day 7 day after ovulation as per the guidelines published by IETS. All embryos were washed at least 3 times in holding medium supplemented with antibiotics and then transferred individually into the left uterine horn of synchronized recipients. All recipients and calves born were tested for brucellosis every 3 weeks. The data were analysed using a two-sample t-test (Minitab statistical software, Minitab Ltd.). Results are shown in Table 1. No difference was observed in the number of embryos collected per flushing and pregnancies established at Day 60. The proportion of pregnancies reaching term from the total number of embryos transferred and from the pregnancies established on Day 60 did not differ from embryos obtained from brucella-infected and control donors. All recipients tested negative for brucellosis during their gestation and until weaning. All calves born were also negative for brucellosis on birth and until weaning and handing over to clients. In conclusion, this is the first study in camels wherein we have demonstrated that invivo-produced embryos from elite dromedary females infected with brucellosis could be collected and transferred to synchronized recipients without transmitting the pathogen to the recipients or offspring. Table 1. Pregnancies established from invivo-produced embryos collected from elite dromedary camels infected with brucellosis Donor group (N) Total number of flushings No. of embryos collected per flushing (n±s.e.m.) Pregnancies on Day 60 (% mean±s.e.m.) Pregnancies reaching term (% mean±s.e.m.) from: Embryos transferred Pregnancies on Day 60 Brucella (10) 30 5.4±0.6 55.1±2.6 44.4±2.3 83.2±3.6 Control (3) 6 5.3±0.6 65.7±6.3 53.3±6.8 80.9±7.7 P-value 0.969 0.173 0.262 0.797

Tesmin, Metallothionein-Like 5, is Required for Spermatogenesis in Mice†

In mammals, more than 2000 genes are specifically or abundantly expressed in testis, but gene knockout studies revealed several are not individually essential for male fertility. Tesmin (Metallothionein-like 5; Mtl5) was originally reported as a testis-specific transcript that encodes a member of the cysteine-rich motif containing metallothionein family. Later studies showed that Tesmin has two splicing variants and both are specifically expressed in male and female germ cells. Herein, we clarified that the long (Tesmin-L) and short (Tesmin-S) transcript forms start expressing from spermatogonia and the spermatocyte stage, respectively, in testis. Furthermore, while Tesmin-deficient female mice are fertile, male mice are infertile due to arrested spermatogenesis at the pachytene stage. We were able to rescue the infertility with a Tesmin-L transgene, where we concluded that TESMIN-L is critical for meiotic completion in spermatogenesis and indispensable for male fertility.

Accelerated Sexual Maturation in Methoprene-Treated Sterile and Fertile Male Queensland Fruit Flies (Diptera: Tephritidae), and Mosquito Larvicide as an Economical and Effective Source of Methoprene

Queensland fruit flies Bactrocera tryoni (‘Q-fly’) have long adult prereproductive development periods, which can present challenges for sterile insect technique (SIT) programs. Holding the sterile flies in release facilities is expensive for control programs. Alternatively, releases of sexually immature males can lead to substantial mortality of sterile males before they mature. Recent studies have reported effectiveness of dietary supplementation with a mosquito larvicide (NOMOZ) that contains S-methoprene, a juvenile hormone analogue, for accelerating sexual development of fertile Q-fly males. However, it is not known whether effects on sterile flies are comparable to effects on fertile flies, or whether effects of methoprene-containing larvicide are comparable to effects of analytical standard methoprene such has been used in most studies. Here we address both knowledge gaps, investigating the effects of analytical standard methoprene and NOMOZ mixed with food and provided for 48 h following emergence on sexual development and longevity of fertile and sterile Q-flies. Compared with controls, fertile and sterile male Q-flies that were provided diets supplemented with methoprene from either source exhibited substantially accelerated sexual development by 2–3 d and longer mating duration. Unlike males, females did not respond to methoprene treatment. Although fertile and sterile flies were generally similar in sexual development and response to methoprene treatment, sterile flies of both sexes tended to have shorter copula duration than fertile flies. Neither methoprene supplements nor sterilization affected longevity of flies. The present study confirms effectiveness of dietary methoprene supplements in accelerating sexual development of both fertile and sterile male (but not female) Q-flies, and also confirms that low-cost mosquito larvicides that contain methoprene can achieve effects similar to those for high-cost analytical grade methoprene as prerelease supplements for Q-fly SIT.

Typology in the fertility of seven Tall coconut (Cocos nucifera L.) populations using artificial pollination traits in field genebank

This study investigates the fertility typology for seven Tall coconut accessions using artificial pollination characters. Ten traits describing fertility were considered for characterizing three groups of coconut individuals per accession. Ten males parental, twenty to forty female parental, and ten progenies were randomly explored per coconut accession. Except for the rate of seeds germination, the outcomes revealed the usefulness of the characters assessed for the discrimination of the accessions studied. The typology of the fertility of the accessions studied is independent of the origins and highlighted two main groups. The first group gathers Laccadive Common Tall (LCT) and West African Tall (WAT and WAT6) accessions including the most fertile male parents and the females showing great production of seed nuts. From this group, the progenies set out a lack of vigour. The second group is constituted of Panama Tall (PNT01 and PNT02), Solomon Island Tall (SIT) and Gazelle Peninsula Tall (GPT) accessions with characters opposite to the previous ones. The investigations of the Tall coconut accessions fertility are fundamental to address the low size of genitors and progenies for respective field genebank regeneration and representative field replanting.

Molecular and cytogenetic analysis of infertile Hakka men with azoospermia and severe oligozoospermia in southern China

Objective To determine the prevalence of chromosome abnormalities and azoospermia factor (AZF) microdeletions in Hakka men with infertility in southern China. Methods Hakka male patients, who received clinical counselling for infertility between August 2016 and October 2017, and fertile male controls, were enrolled into this retrospective study. Patients diagnosed with infertility and controls underwent cytogenetic analysis by standard G-banding; AZF microdeletions were examined by multiplex polymerase chain reaction and capillary electrophoresis. Results Out of 918 male patients who received fertility counselling, 57 were diagnosed with infertility due to azoospermia or severe oligozoospermia. Of these infertile patients, 22.81% (13/57) carried chromosome abnormalities, with 47, XXY being the most common abnormal karyotype. In addition, 36.84% (21/57) presented with Y chromosome microdeletions, most frequently in the complete AZFc and partial AZFc region. Duplication of the AZFc region was found in three patients. No AZF microdeletions were found in 60 fertile male controls. Conclusion The high AZF microdeletion frequency in the current Hakka population suggests that AZF microdeletion analysis is essential in fertility screening, and combined with cytogenetic analysis, may influence the choice of assisted reproductive techniques and reduce the risk of inherited genetic disease.