Experimental Study of the Effects of Changes in Arteriovenous Pressure Difference on the Survival Area of a Reverse Island Flap

In this article, the effect of the change of arteriovenous pressure on the survival area of reverse island flap was studied. The vein and saphenous artery in rabbits were selected to design the reverse island flap experimental model. Rabbits were divided into 4 groups: A: Control Group; B: Part anastomosis of the saphenous artery group; C: Part anastomosis of the vein group; and D: No superficial veins group. After surgery, for all four groups, flaps were assessed by general observation, radionuclide scans for the survival rate, and histology. We found that the survival rate of flaps in Group B was overtop than the other three groups (P < 0.05). The radioactive material (RM) in group B could be seen clearly, whereas the RM in Groups A, C, and D existed transiently. At ten days post-operation. Group B had more capillary regeneration and blood cells contrast to the other three groups. Increasing blood supply can improve the survival rate of flaps, but simply promoting venous return has no significant effect on improving the survival rate of flaps.

MAPKs Are Highly Abundant but Do Not Contribute to α1-Adrenergic Contraction of Rat Saphenous Arteries in the Early Postnatal Period

Previously, the abundance of p42/44 and p38 MAPK proteins had been shown to be higher in arteries of 1- to 2-week-old compared to 2- to 3-month-old rats. However, the role of MAPKs in vascular tone regulation in early ontogenesis remains largely unexplored. We tested the hypothesis that the contribution of p42/44 and p38 MAPKs to the contraction of peripheral arteries is higher in the early postnatal period compared to adulthood. Saphenous arteries of 1- to 2-week-old and 2- to 3-month-old rats were studied using wire myography and western blotting. The α1-adrenoceptor agonist methoxamine did not increase the phosphorylation level of p38 MAPK in either 1- to 2-week-old or 2- to 3-month-old rats. Accordingly, inhibition of p38 MAPK did not affect arterial contraction to methoxamine in either age group. Methoxamine increased the phosphorylation level of p42/44 MAPKs in arteries of 2- to 3-month-old and of p44 MAPK in 1- to 2-week-old rats. Inhibition of p42/44 MAPKs reduced methoxamine-induced contractions in arteries of 2- to 3-month-old, but not 1- to 2-week-old rats. Thus, despite a high abundance in arterial tissue, p38 and p42/44 MAPKs do not regulate contraction of the saphenous artery in the early postnatal period. However, p42/44 MAPK activity contributes to arterial contractions in adult rats.

Saphenous Artery Perforator/Propeller Flap in the Reconstruction of Perigenual Soft Tissue Defects

Introduction In this article, we investigate the saphenous artery perforator propeller flap for the reconstruction of perigenual defects. We describe the anatomy of the saphenous artery and the method of raising a perforator propeller flap based on our study and early clinical experience. Aim To assess the effectiveness of the saphenous artery perforator propeller flap in the reconstruction of perigenual defects. Materials and Methods A preliminary cadaver dissection and injection study was conducted to discern the anatomical details of the perforator system of the saphenous vessel in 35 cadaveric specimens in 18 fresh cadavers. From March 2016 to March 2018, 16 clinical cases (5 females and 11 males, in the average age group of 33.5 years) with perigenual defects were reconstructed with saphenous artery perforator propeller flap. They were followed up for an average period of 12.5 months. Results Cadaver study established anterior sartorial perforators as the dominant system of the saphenous vessel and brought to the forefront the type 2 blood supply of the saphenous nerve. All patients had well-settled flaps at the end of the follow-up period, with good return of knee function. Conclusion Saphenous artery perforator propeller flap is a viable option for the reconstruction of defects around the knee joint. Level of Evidence Type V, therapeutic study.

Using the Saphenous Artery and Great Saphenous Vein Combined with Anterolateral Thigh Flap to Treat Skin Defects after Amputation

Background Aim of this study was to determine the feasibility of using the saphenous artery (SA) and great saphenous vein (GSV) as recipient vessels, combined with anterolateral thigh (ALT) flap, in the treatment of skin defects after lower limb amputation. Methods From June 2015 to June 2017, 12 patients (average age, 33.5 years; range, 14–56 years; males, 9; female, 3) with large skin defects and symptoms of bone exposure in the proximal lower extremity were included in our study. The patients underwent emergency treatment and multiple debridement combined with vacuum sealing drainage therapy, followed by free flap surgery using the SA and GSV as recipient vessels, and ALT to cover the wound. Results All 12 patients who underwent free flap surgery survived, but two patients had distal flap necrosis, which, however, was salvaged with conservative measures. All patients were satisfied with the postoperative outcome at the 3 and 6-month follow-up. Conclusion The SA and GSV can be used as recipient vessels, combined with ALT, to treat skin defects after lower limb amputation.

A New Supermicrosurgery Training Model of Saphenous Artery and Great Saphenous Vein Anastomosis for Development of Advanced Microsurgical Skills

Background This study aimed to confirm the feasibility and reliability of saphenous artery (SA) and great saphenous vein (GSV) anastomosis as a new supermicrosurgery training model and to compare the one-way-up anastomosis with the currently used end-to-end anastomosis technique. Methods Twenty supermicrosurgical anastomoses were performed in 10 Sprague Dawley rats. The external diameters of SA and GSV were measured using Leica LAS EZ software. The right-side SA and GSV anastomoses were performed using the standard end-to-end anastomosis technique. The left-side SA and GSV anastomoses were performed using the one-way-up technique with 11–0 monofilament-interrupted sutures. The duration of the surgery, patency rates, and technical challenges of the two anastomoses methods were compared. Results The mean external diameters of SA and GSV were 0.273 ± 0.03 and 0.291 ± 0.02 mm, respectively, which qualify these vessels for supermicrosurgical training. The vessels were easily accessible and both anastomosis techniques were feasible. The one-way-up technique was proven to be faster as compared with the end-to-end anastomosis technique (artery: 34 ± 2.55 vs. 40.4 ± 2.97 minutes, p = 0.02; and vein: 37 ± 4.85 vs. 44 ± 2.35 minutes, p = 0.05, respectively). Short-term patency rates for arteries and veins were 100% for both techniques. Seven-day anastomosis patency rates for arteries and veins were 80 and 100% for the end-to-end technique and 100 and 80% for the one-way-up technique, respectively. Conclusions We confirmed that saphenous pedicle is suitable for creating a supermicrosurgery training model for practicing the ultrafine motor skills. To the best of our knowledge, this is the first report on supermicrosurgery of SA and GSV in the rat model.