Scientific justification of the formula composition of biotechnological form of BAA with directed functional properties

For the prevention and treatment of gastrointestinal diseases, the microbiome of the living organism is of great importance. The human health depends on quantitative and qualitative composition of microbiota. Under the influence of factors of internal and external environment there is a change in the indigenous microflora. For correction, prevention and regulation of intestinal microbiocenosis within the framework of the biotechnology program, biologically active additive (BAA) is proposed, the main components of which are selective biomass bacteria, prebiotics, peptide ultralisates. As substances that perform the function of coenzymes, thiamine (vitamin B1) and pyridoxine (vitamin B6) are used in the production of this additive. The novelty of the technology is the composition and structure of solid gelatin capsules, ensuring maximum safety of biologically active ingredients and delivery of them to cells. Regulated quality indicators have been established. Sanitary-hygienic and sanitary-toxicological indicators of the BAA comply with the requirements of TR CU 021/2011 “On safety of food products”. Clinical approbation of the developed additive was carried out by volunteers with detected disorders of intestinal microbiota. BAA was taken 1 capsule 2 times a day in conjunction with other drugs regulating bowel biocenosis. At the end of dietary therapy, the microbiota recovery was observed in 90% of cases. Technical documentation has been approved for the new product, recommendations for application have been developed.

Pengaruh Medium Yang Tercemar Organoklorin (Endosulfan) Terhadap Kandungan Agar Dan Morfologi Thallus Gracilaria verrucosa [Effect Of Organochlorines (Endosulfan) Contaminated Medium On Content Of Gelatin And Thallus Morphology Gracilaria verrucosa]

Gracilaria verrucosa seaweed is one of the high economic value because its potential as raw with the good content of more agarose than agaropektin so that it can produce gelatin to form strong and solid gelatin. The content range is from 12-48% depending on the class species Rhodophyceaea. Organochlorine residues (Endosulfan) come down to sea by the use of Organochlorine continuously, finally settles on the ground and carried by the flow of rain. Organochlorine residues (Endosulfan) excess in aquatic may influence biota so that can degrade and change the content that Thallus morphology on Gracilaria verrucosa. This research aims to determine the content of that reduction and morphological changes of Gracilaria verrucosa thallus on organochlorine contaminated medium (Endosulfan). This research method s experimental, while the design of the study is a Rancangan Acak Lengkap (RAL) with 5 treatments and 4 replications. The main parameters in this research that content of gelatin and thallus morphology Gracilaria verrucosa, while supporting parameters in this study consisted of: water quality medium. The results showed that the organochlorine contaminated medium (Endosulfan) significantly influenced (p <0.05) of the gelatin content. Ingredients that are highest in treatment A (41.2%). Content that is second highest on treatments B (35.55%). Ingredients that are the third highest in treatment C (22.02%), followed by treatment D (16.72%) and treatment E (13%) showed the lowest gelatin. Each treatment showed significantly different between treatments. Thallus morphology Observations show differences in the thickness of the thallus epidermis such as the dose of pollutant. The greater the dose of organochlorines (Endosulfan) given the Thallus epidermis G. verrucosa diminution.

Characterization of proteic content of gelatins

<p style="text-align: justify;">Enological gelatins are currently used in the red wines collage. We found those gelatins added with particular composants in white wines treatment.</p><p style="text-align: justify;">The Kjeldahl method recommanded by the Enology Codex gives the global concentration in nitrogen containing compounds. In order to characterize the protein content of oenological gelatins, simple and rapid determinations, based on colorimetric reactions have been selected. The proteic concentrations have been determined from commercial kits SIGMA : Biuret and Ninhydrin reactions are both concerned. They have a good repetability (variation coefficient &lt; 6 p. cent). With Biuret, hot soluble gelatins have high concentrations, instead cold soluble and liquid gelatins have light concentrations. With Ninhydrin, results are reversed. A coefficient corresponding to the ratio of those two methods allows to characterize the hydrolyse degree of proteic chains : C = [B] / [N], Gelatins can be classified in two groups : C &lt; 35 for cold soluble and liquid gelatins , C &gt; 35 for hot soluble gelatins.</p><p style="text-align: justify;">Otherwise, results obtained in Kjeldahl and Biuret methods are proportional. In fact, the authors suggest a limit proteic concentration expressed in mg BSA/g solid gelatin : 750 mg BSA /g corresponding to the standard decided by the Codex, 14 % nitrogen minimum.</p>

A NEW METHOD FOR STREAKING BLOOD AGAR PLATES

A NEW METHOD for the streaking of agar plates and the obtaining of isolated bacterial colonies on the agar surface is presented. The method involves the use of thin paper discs which are placed on the agar and cover the entire surface. The disc has linear slits of narrow width allowing optimum amounts of the specimen to be distributed through the paper and onto the agar surface when the paper is rubbed with a swab bearing the inoculum. Robert Koch in his epochal publication of 18811,2 introducing the pure culture technique into bacteriology first described the use of solid gelatin media. In this publication he described the use of a platinum wire for the streaking of specimens onto the gelatin surface. Hesse in 1884, while working in Koch's laboratory, discovered the use of agar as a replacement for gelatin.3 Petri,4 in 1887, also working in Koch's laboratory, invented the culture dish which bears his name. It is notable that, with minor exceptions, the technique of streaking specimens onto agar plates by means of a wire loop has remained virtually unchanged to this date. Throat cultures of patients in St. Francis Hospital, Evanston, Illinois; Lutheran General Hospital, Park Ridge; and the University of Illinois Hospital, Chicago, were studied. Studies were also made of throat cultures in the laboratories of the Evanston Board of Health. A total of 550 throat cultures was obtained. Cultures were made m 450 cases using the Iso-Disc technique only, and 100 cultures were studied in duplicate with the Iso-Disc method and the conventional wire loop method.