Detection of miRNA using a surface plasmon resonance biosensor and antibody amplification

MiRNAs are non-coding RNA molecules that control biological functions by reducing the translation of target proteins when binding to the mRNA. Alterations of the miRNA expression profile affect the cell metabolism, which can lead to distinctive disease patterns thus suggesting miRNA as an interesting biomarker. Here we present a SPR biosensor that utilizes disposable, injection-molded sensor chip/microfluidic hybrids combined with a lateral imaging optical system for parallel analysis of three one-dimensional spot arrays to detect miRNA-93. Using a RNA-DNA-hybrid antibody for signal enhancement we could reach a limit of detection of 10 pmol/l.

Increased Kappa/Lambda Hybrid Antibody in Serum Is a Novel Biomarker Related to Disease Activity and Inflammation in Rheumatoid Arthritis

Theκ/λhybrid antibodies in normal human serum were reported recently, but their clinical relevance has not yet been explored. Rheumatoid arthritis (RA) is one of the major joint diseases, and the early diagnosis and treatment of RA remain a challenge. Here, we developed a double-sandwich enzyme-linked immunosorbent assay system to quantify relative serumκ/λhybrid antibody levels in RA patients, osteoarthritis (OA) patients, and healthy controls (HC) in order to assess their potential use as a serological biomarker of early disease and clinical activity and to preliminarily investigate their immunomodulatory roles in RA. Surprisingly, we found thatκ/λhybrid antibody was markedly increased in both early and established RA. Serumκ/λhybrid antibody levels were significantly correlated with clinical indexes and inflammatory markers in RA. Further analysis showed a positive correlation betweenκ/λhybrid antibody levels and the 28-joint disease activity score (DAS28). In conclusion, serumκ/λhybrid antibodies in RA were identified for the first time. High levels ofκ/λhybrid antibody may be a useful tool in distinguishing early RA from OA and HC. We suggestκ/λhybrid antibody as a marker for disease activity. The increasedκ/λhybrid antibodies were associated with inflammatory conditions in RA.