sugar recognition
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2021 ◽  
Vol 69 (6) ◽  
pp. 526-528
Author(s):  
Misaki Nakano ◽  
Rikako Nakamura ◽  
Yuto Sumida ◽  
Kazunori Nagao ◽  
Taniyuki Furuyama ◽  
...  

2019 ◽  
Vol 7 ◽  
Author(s):  
Ko Sugita ◽  
Yuji Tsuchido ◽  
Chisato Kasahara ◽  
Maria Antonietta Casulli ◽  
Shoji Fujiwara ◽  
...  

2019 ◽  
Vol 55 (2) ◽  
pp. 1-4
Author(s):  
T. Goto ◽  
Y. Kitamoto ◽  
K. Matsui ◽  
H. Kuroe ◽  
A. Endo ◽  
...  

2017 ◽  
Vol 241 ◽  
pp. 116-123 ◽  
Author(s):  
Dieric S. Abreu ◽  
Ticyano P. Sousa ◽  
Caio B. Castro ◽  
Mayara N.V. Sousa ◽  
Thiago T. Silva ◽  
...  

Author(s):  
Ayman Attigani ◽  
Lifang Sun ◽  
Qing Wang ◽  
Yadan Liu ◽  
Dingping Bai ◽  
...  

Cellulases are produced by microorganisms that grow on cellulose biomass. Here, a cellulase, Cel10, was identified in a strain ofKlebsiella pneumoniaeisolated from Chinese bamboo rat gut. Analysis of substrate specificity showed that Cel10 is able to hydrolyze amorphous carboxymethyl cellulose (CMC) and crystalline forms of cellulose (Avicel and xylan) but is unable to hydrolyzep-nitrophenol β-D-glucopyranoside (p-NPG), proving that Cel10 is an endoglucanase. A phylogenetic tree analysis indicates that Cel10 belongs to the glycoside hydrolase 8 (GH8) subfamily. In order to further understanding of its substrate specificity, the structure of Cel10 was solved by molecular replacement and refined to 1.76 Å resolution. The overall fold is distinct from those of most other enzymes belonging to the GH8 subfamily. Although it forms the typical (α/α)6-barrel motif fold, likeAcetobacterxylinumCMCax, one helix is missing. Structural comparisons withClostridium thermocellumCelA (CtCelA), the best characterized GH8 endoglucanase, revealed that sugar-recognition subsite −3 is completely missing in Cel10. The absence of this subsite correlates to a more open substrate-binding cleft on the cellooligosaccharide reducing-end side.


Biochemistry ◽  
2015 ◽  
Vol 54 (38) ◽  
pp. 5999-6008 ◽  
Author(s):  
Hayley J. Schultz ◽  
Andrea M. Gochi ◽  
Hannah E. Chia ◽  
Alexie L. Ogonowsky ◽  
Sharon Chiang ◽  
...  

2015 ◽  
Vol 73 (7) ◽  
pp. ftv054 ◽  
Author(s):  
Keita Miyata ◽  
Tomonori Suzuki ◽  
Shintaro Hayashi ◽  
Shin-Ichiro Miyashita ◽  
Tohru Ohyama ◽  
...  

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