Antimicrobial Activity of Medicinal Compounds Produced by Fungal Degradation of Soybean Oil Using Lignocellulose Biomass as Surface

Methyl esters were produced by fungal degradation of soybean oil using banana leaves as surface. Analysis of the products also revealed medicinal compounds (Benzyl Benzoate obtained from the banana leaves used as surface for the fungal degradation of the soybean oil and 1, 2-Benzene dicarboxylic acid dioctyl ester obtained from the soybean oil impurities). Microorganism activity test was carried out on the medicinal compounds to confirm their antibacterial and antifungal activity. The result showed that both had antibacterial and antifungal activity as shown by the zones of inhibition and as a result can find useful applications in food processing and preservation as well as agriculture as pest/disease control agents.

Water Photo-Electrooxidation Using Mats of TiO2 Nanorods, Surface Sensitized by a Metal–Organic Framework of Nickel and 1,2-Benzene Dicarboxylic Acid

Photoanodes comprising a transparent glass substrate coated with a thin conductive film of fluorine-doped tin oxide (FTO) and a thin layer of a photoactive phase have been fabricated and tested with regard to the photo-electro-oxidation of water into molecular oxygen. The photoactive layer was made of a mat of TiO2 nanorods (TDNRs) of micrometric thickness. Individual nanorods were successfully photosensitized with nanoparticles of a metal–organic framework (MOF) of nickel and 1,2-benzene dicarboxylic acid (BDCA). Detailed microstructural information was obtained from SEM and TEM analysis. The chemical composition of the active layer was determined by XRD, XPS and FTIR analysis. Optical properties were determined by UV–Vis spectroscopy. The water photooxidation activity was evaluated by linear sweep voltammetry and the robustness was assessed by chrono-amperometry. The OER (oxygen evolution reaction) photo-activity of these photoelectrodes was found to be directly related to the amount of MOF deposited on the TiO2 nanorods, and was therefore maximized by adjusting the MOF content. The microscopic reaction mechanism which controls the photoactivity of these photoelectrodes was analyzed by photo-electrochemical impedance spectroscopy. Microscopic rate parameters are reported. These results contribute to the development and characterization of MOF-sensitized OER photoanodes.

Characteristics of the root exudate release system of typical plants in plateau lakeside wetland under phosphorus stress conditions

In this study, the root exudates of wetland plants, Pistia stratiotes, black algae, and Cyperus alternifolius, exposed to six phosphorus concentration gradients (0, 0.2, 1, 5, 10, and 20 mg/L) were characterized. The experimental seedlings were cultivated in Hoagland solutions, which were then extracted, decompressed, and concentrated with CH2Cl2; subsequently, a gas chromatography-mass spectrometry (GC-MS) analysis was performed to study the root exudates effects under different phosphorus concentrations. Results showed the existence of several organic compounds, such as alkanes, esters, alcohols, amines, benzene, and acids (phthalic acid, cycloheptasiloxane, benzoic acid, and cyclopentasiloxane) in the root exudates of the wetland plants. The relative contents of phthalate, benzene dicarboxylic acid, and cyclohexasiloxane in the root exudates first increased, and then decreased, with the change in phosphorus concentration. The relative contents of three compounds in Pistia were the highest at 1 mg/L of phosphorus, and the lowest relative contents of phthalic acid and benzene dicarboxylic acid were observed at 20 mg/L of phosphorus. However, the relative content of cyclohexasiloxane was the lowest in the absence of P stress. In black algae, the relative contents of the three compounds were 36.66, 16.24, and 14.61%, respectively. The relative content of cyclohexasiloxane in the black algae first decreased and then increased, with its lowest relative content occurring at 5 mg/L of phosphorus and the highest at 10 mg/L of phosphorus. In Cyperus alternifolius, the highest relative concentrations of the four compounds: phthalic acid, dimethyl phthalate, octadecane, and diphenyl sulfone in Cyperus were observed at 5 mg/L phosphorus and the lowest at 10 mg/L phosphorus.

Antifungal activity and GC-MS detection of leaves and roots parts of Chenopodium album extract against some phytopathogenic fungi

Antifungal potential of leaves and roots parts of Chenopodium album was evaluated against five phytopathogenic fungi including Alternaria alternata, Fusarium solani, Rhizoctonia solani, Pythium aphanidermatum and Sclerotinia sclerotium using four concentrations viz. 0, 5, 10 and 15% of leaves and roots water extract led to significantly reduced of fungi mycelial growth. GC-MS analysis of leaves and roots water extract showed the presence of 7 compounds. 2(3H)-furanone, dihydro-4,4-dimethyl; 9-octadecenoic acid (Z), methyl ester; 9,12-octadecenoic acid (Z), methyl ester; 6-methylene bicyclo (3.2.0) hept-3-en-2-one., 1,2-benzene dicarboxylic acid, mono (2-ethylhexyl) ester and hexadecanoic acid, methyl ester. Five phytopathogenic fungi could be managed and controlled by the water leaves and roots extract of C. album. The antifungal activity of this extract was possible could be used to control a wide range of fungi.

Secondary Metabolites and Antioxidant Potentials of Axis Sea Bamboo (Isis hippuris)

Secondary metabolites of the axis section (modulla skeleton) of sea bamboo (Isis hippuris) were identified by GC-MS. The dominant compounds using GC-MS in the ethanol fraction were hexanedioic acid (11.85%), acetamide (9.46%), n-hexadecanoic acid (9.22%) and thiosulfuric acid (7.22%). On the other side, the dominant compounds of the test results in the ethyl acetate fraction were 7-oxabicyclo[4.1.0] heptanes (28.27%), 1,2- benzene dicarboxylic acid (14.77%), cis-8-(N-pyrrolidyl)-(2,2,5,5-tetradeutero)bicyclo[4.3.0]nona-3,7-diene (9.98%), hexahydropyridine (7,86%). The dominant compounds of the n-hexane fraction were hexanedioic acid (41.99%), azetidine with a peak number of 40 (9.98%), 1-octadecene (8,36%). Antioxidant activities were also evaluated by DPPH scavenging assay. The ethanol, n-hexane and ethyl acetate fractions showed total flavonoid contents of 7.86% ± 0.12, 12.97% ± 0.36, 1.88% ± 0.26, respectively and the IC50 in the antioxidant assay were 480.25±74,74, 469.50±19,13, 3221.07±138,69 respectively. Dhaka Univ. J. Pharm. Sci. 18(1): 13-20, 2019 (June)

GC-MS Analysis of the Ethanolic Extract of the whole Plant Drosera indica L.

Aim: The present study is carried out to explore the phytoconstituents present in the ethanolic extract of the whole plant Drosera indica L. by GC-MS analysis. Methods: The ethanolic extract of the whole plant of Drosera indica L. is used for the GC-MS analysis. JEOL GCMATE II GC-MS (Agilent Technologies 6890 N Network GC system for gas chromatography) is used for the analysis. The compounds are identified by the gas chromatography coupled with the mass spectrometry. The molecular weight and structure of the compounds of test materials are ascertained by interpretation of the mass spectrum of GC-MS using the database of National Institute Standard and Technology (NIST). Results: GC-MS analysis of Drosera indica L. reveal the presence of the nine biological active compounds which include phytol, 4 methyl-4-nonadecene, 4 ’ ,5,7-Trihydroxyisoflavone,1,2-Benzene dicarboxylic acid, bis [2-methyl propyl] ester, Tetra decanoic acid, Eicosane, 2,6,10,14,18–pentamethyl, Tetracosane, Lochnerine and methyl–n-hexadecylketone. Conclusion: The results specify that the ethanolic extract of the whole plant, Drosera indica contains various bioactive compounds and therefore has various medicinal properties which can be used for the treatment of various diseases.