hamster ova
Recently Published Documents


TOTAL DOCUMENTS

93
(FIVE YEARS 0)

H-INDEX

28
(FIVE YEARS 0)

2015 ◽  
Vol 21 (1) ◽  
pp. 118
Author(s):  
S.D. Kharche ◽  
S.K. Jindal ◽  
A.K. Goel ◽  
Satish Kumar ◽  
Chetna Gangwar ◽  
...  
Keyword(s):  

2000 ◽  
Vol 54 (6) ◽  
pp. 827-834 ◽  
Author(s):  
W. Ji ◽  
B.D. Bavister
Keyword(s):  

1995 ◽  
Vol 14 (16) ◽  
pp. 1807-1818 ◽  
Author(s):  
Markus Abt ◽  
Erwin Strehler ◽  
Bernd Rosenbusch ◽  
Karl Sterzik

Zygote ◽  
1995 ◽  
Vol 3 (2) ◽  
pp. 163-170 ◽  
Author(s):  
Kendall Ash ◽  
Trish Berger ◽  
Catherine M. Horner ◽  
C. Chris Calvert

SummarySperm plasma membrane (PM) proteins that demonstrate affinity for egg PM preparations have the potential to be biologically important during sperm-egg binding and/or fusion. In this study four such proteins have been identified. To provide quantitative evidence for possible biological function, the large natural variation among different porcine sperm populations with regard to their ability to interact with the egg was compared with the relative binding of egg PM material to individual proteins. An aliquot from each of 24 porcine ejaculates was evaluated by the zona-free hamster ova bioassay and the remainder processed to yield sperm PM vesicles. Aliquots of sperm PM were solubilised, separated by SDS-PAGE, western blotted and probed with partially purified, biotinylated egg PM protein. Bound egg PM proteins were visualised on western blots by an avidin/biotin/horseradish peroxidase system and analysed by scanning laser densitometry. Four sperm PM proteins (62, 39, 27 and 7 kDa estimated molecular mass) were the predominant binders of egg PM. The amount of egg PM bound to the 62 kDa protein was significantly correlated with the ability of sperm from the 24 ejaculates to penetrate zona-free hamster ova (percentage of ova penetrated, p = 0.01, R = 0.65; number of penetrated sperm per ovum, p = 0.02, R = 0.63)


Sign in / Sign up

Export Citation Format

Share Document