The influence of Semax on the activity of brain mitochondria succinate dehydrogenase in experimental bilateral photochemical ischemic damage to the prefrontal cortex

Ишемическое повреждение префронтальной коры головного мозга сопровождается ранним и прогрессирующим во времени снижением активности сукцинатдегидрогеназы (СДГ) митохондрий перифокальной зоны. Суточное дробное введение синтетического аналога фрагмента АКТГ - Семакса в дозе 25 мкг/кг сопровождается нормализацией активности СДГ через 24 ч после фототромбоза коры. Исследование показало, что определение активности СДГ является информативным методом, позволяющим оценить тяжесть ишемического повреждения головного мозга и эффективность нейропротекторной терапии. Ischemic damage to the prefrontal cortex is accompanied by early and progressive decrease in the succinate dehydrogenase (SDH) activity of the mitochondria of the perifocal area. The daily fractional administration of a synthetic analogue of the ACTH fragment - Semax at a dose of 25 µg / kg is accompanied by normalization of the activity of SDH 24 hours after photothrombosis of the cortex. The study showed that the determination of the activity of SDH is an informative method to assess the severity of ischemic brain damage and the effectiveness of neuroprotective therapy.

ISOLATION, AMINO ACID SEQUENCE AND ACTION OF GUINEA-PIG ACTH ON ALDOSTERONE PRODUCTION BY GLOMERULOSA CELLS

ABSTRACT Though minor sequence differences between-species have been reported for adrenocorticotrophin, ACTH(1–39), the steroidogenic moiety ACTH(1–24) has appeared invariant in mammals. We here report the isolation, purification and amino acid sequencing of guinea-pig (GP) ACTH in which Pro24 is replaced by [ill] Ala24, and the demonstration that GP-ACTH stimulates aldosterone production to maximal levels well above those seen with human ACTH(1–39) or Synacthen, ACTH(1–24) amide, the synthetic ACTH fragment widely used for diagnostic and therapeutic purposes.