Efficacy of Methylobacterium oryzae Supplemented Sargassum wightii Seaweed Liquid Fertilizer on Chilly and Tomato Plant Growth

Liquid seaweed fertilizer gaining interest due to their use in crop productivity. The present study was designed to evaluate the impact of Methylobacterium oryzae amended liquid seaweed fertilizer on domestic plants like chilly and tomato. The Sargassum wightii methanolic extract was maximum (137 mg/g) among other solvent tested. Extract showed 0.456mg/mL, 1.587 mg/mL, 0.78 mg/g, 5.27 mg/g, 0.63 mg/mL, 0.98 mg/mL, 0.285 g/mL and 0.546 μg/mL of total phenolic, total flavonoids, total chlorophyll, total carotenoids, total protein, total carbohydrates, total lipids and total aminoacids respectively. The maximum survival of Methylobacterium oryzae was observed at 40 % of Sargassum wightii SLF extract with 3% Methylobacterium oryzae culture concentration and has viability of 750 × 106 CFU/l after 6 months. Impact of foliar sprayed liquid fertilizer on the chlorophyll, internode and shoot length had been promising over seed soaked.

Phytochemical Research of Methylobacterium Species Seaweed Extraction Chilly and Tomato Plant Growth

Chemical fertilizers possess health risks and soil pollution, in addition to these very expensive and significantly higher production costs. Seaweed liquid fertilizer is proved to be the better alternative to nourish plants. Hence, the present study focused on the alternate from Sargassum Species seaweed. Extraction of sea weed with water, ethanol, chloroform, methanol, petroleum ether and ethyl acetate were tested for the efficiency with and without Microbial supplementation. Methanolic extract found to show higher protein components (670 µg/ml), carbohydrate (98µg/ml), total phenolic (0.256 mg/ml), chlorophyll (0.260 mg/ml) and lower levels of lipids (0.650gm/10mL), carotenoids (0.057mg/ml), and Total Flavonoid (1.087 mg/ml). Bacterial supplementation of seaweed with Methylobacterium species was found to influence plant growth positively.

Effect of Seaweed Liquid Fertilizer on Antioxidant and Enzyme Activity of Different Vegetables Seeds

The aim of the study was to find the antioxidant activity and enzyme activity of catalase and peroxidase of vegetable plants. The results indicated that the use of seaweed liquid fertilizer can enhance the antioxidant activity of Solanum melongena L., Lycopersicon esculentum Mill., Capsicum annuum L., Brassica oleracea var. Capitata L. and Allium cepa L. The 1,1-diphenyl-2-picrylhydrazyl (DPPH) assays were used to determine the antioxidant properties of seaweeds by measuring the decrease in absorbance at 517 nm. The DPPH activity was highest in brown seaweed liquid fertilizer. This study implied that impacts on vegetable plantlets by seaweed liquid fertilizer extracted with enzymes is better in brown seaweed liquid fertilizer as compared to control.

Phytochemical screening of Lycopersicum esculentum Mill. treated with seaweed liquid fertilizer

Premise of the study: The objective of the work is to manitor the phytochemicals present in methanolic extract of Lycopersicum esculentum after treatment with different concentrations of seaweed liquid fertilizer (SLF). Methods: In this study SLF treated L.esculentum were characterized through gas chromatography and mass spectroscopy to identify the phytochemicals constituents and functional groups of the compounds. Results: Preliminary phytochemical analysis revealed the presence of alkaloids, phenols, carbohydrates, saponins, glycosides, flavonoids, terpinoids. Among the thirty nine phytochemical constituents octa decanoic acid, stigmasterol, hexa decanoic acid and Vitamin E are relatively present in higher yield. The active phytochemical compounds and their constituents were identified with gas chromatography and mass spectrometry. The retention time, percentage of area, molecular weight and chemical formula of phytochemical compounds were determined with help of NIST08 and WILEY8 libraries. The functional groups identified from the spectrum techniques are alcohol, aldehyde, iso cyanides, alkyl compound and chloro compounds. Accordingly hexadecanic acid, beta.-amyrin, gamma sisterol, octodecanic acid, phytol, stigmasterol, vitamin E, lupeol were derived from SLF treated i.e. compared to control plants. Conclusion: The results of this study offer a platform of using SLF treated L.esculentum has an alternative source for various biological studies and it can be used as functional and pharmaceutical purposes. Keywords: SLF, Lycopersicum esculentum, Phytochemical, GC-MS and FTIR

Preliminary studies on the eff ect of bioactive substances of Hypnea musciformis (Wulf.) Lamour. on the growth of seedlings in green gram, Vigna radiata L.

<p>The effect of seaweed liquid fertilizer (SLF) of Hypnea musciformis (wulf.) Lamour.was tested at different concentrations (1, 2, 3 and 4 g/ml) on seed germination and biochemical constituents of Vigna radiata L. The extracts promoted seed germination at lower levels of SLF application. The seeds treated with, 2 g/ ml concentration showed maximum seed germination, radicle length, hypocotyl length, seedling length, fresh weight, phytomass, productivity, phytotoxicity, and photosynthetic pigments like Chlorophyll A, B, and total chlorophyll. Moreover, the different extracts of seaweed were subjected to phytochemical screening. All the<br />tested compounds (tannins, alkaloids, saponins, glycosides, and steroids) were present in varied degrees in Hypnea. The result of the present study can help in the identification and selection of raw materials in agriculture and drug industry.</p>